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Prospective Evaluation of CD45RA+/CCR7- Effector Memory T (T(EMRA)) Cell Subsets in Patients with Primary and Secondary Brain Tumors during Radiotherapy of the Brain within the Scope of the Prospective Glio-CMV-01 Clinical Trial

Radiotherapy (RT) of the brain is a common treatment for patients with high-grade gliomas and brain metastases. It has previously been shown that reactivation of cytomegalovirus (CMV) frequently occurs during RT of the brain. This causes neurological decline, demands antiviral treatment, and is asso...

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Autores principales: Scheer, Ilka, Becker, Ina, Schmitter, Charlotte, Semrau, Sabine, Fietkau, Rainer, Gaipl, Udo S., Frey, Benjamin, Donaubauer, Anna-Jasmina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9954596/
https://www.ncbi.nlm.nih.gov/pubmed/36831183
http://dx.doi.org/10.3390/cells12040516
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author Scheer, Ilka
Becker, Ina
Schmitter, Charlotte
Semrau, Sabine
Fietkau, Rainer
Gaipl, Udo S.
Frey, Benjamin
Donaubauer, Anna-Jasmina
author_facet Scheer, Ilka
Becker, Ina
Schmitter, Charlotte
Semrau, Sabine
Fietkau, Rainer
Gaipl, Udo S.
Frey, Benjamin
Donaubauer, Anna-Jasmina
author_sort Scheer, Ilka
collection PubMed
description Radiotherapy (RT) of the brain is a common treatment for patients with high-grade gliomas and brain metastases. It has previously been shown that reactivation of cytomegalovirus (CMV) frequently occurs during RT of the brain. This causes neurological decline, demands antiviral treatment, and is associated with a worse prognosis. CMV-specific T cells are characterized by a differentiated effector memory phenotype and CD45RA+ CCR7- effector memory T (T(EMRA)) cells were shown to be enriched in CMV seropositive individuals. In this study, we investigated the distribution of T(EMRA) cells and their subsets in the peripheral blood of healthy donors and, for the first time, prospectively within the scope of the prospective Glio-CMV-01 clinical trial of patients with high-grade glioma and brain metastases during radiation therapy as a potential predictive marker. First, we developed a multicolor flow cytometry-based assay to monitor the frequency and distribution of T(EMRA) cells in a longitudinal manner. The CMV serostatus and age were considered as influencing factors. We revealed that patients who had a reactivation of CMV have significantly higher amounts of CD8+ T(EMRA) cells. Further, the distribution of the subsets of T(EMRA) cells based on the expression of CD27, CD28, and CD57 is highly dependent on the CMV serostatus. We conclude that the percentage of CD8+ T(EMRA) cells out of all CD8+ T cells has the potential to serve as a biomarker for predicting the risk of CMV reactivation during RT of the brain. Furthermore, this study highlights the importance of taking the CMV serostatus into account when analyzing T(EMRA) cells and their subsets.
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spelling pubmed-99545962023-02-25 Prospective Evaluation of CD45RA+/CCR7- Effector Memory T (T(EMRA)) Cell Subsets in Patients with Primary and Secondary Brain Tumors during Radiotherapy of the Brain within the Scope of the Prospective Glio-CMV-01 Clinical Trial Scheer, Ilka Becker, Ina Schmitter, Charlotte Semrau, Sabine Fietkau, Rainer Gaipl, Udo S. Frey, Benjamin Donaubauer, Anna-Jasmina Cells Article Radiotherapy (RT) of the brain is a common treatment for patients with high-grade gliomas and brain metastases. It has previously been shown that reactivation of cytomegalovirus (CMV) frequently occurs during RT of the brain. This causes neurological decline, demands antiviral treatment, and is associated with a worse prognosis. CMV-specific T cells are characterized by a differentiated effector memory phenotype and CD45RA+ CCR7- effector memory T (T(EMRA)) cells were shown to be enriched in CMV seropositive individuals. In this study, we investigated the distribution of T(EMRA) cells and their subsets in the peripheral blood of healthy donors and, for the first time, prospectively within the scope of the prospective Glio-CMV-01 clinical trial of patients with high-grade glioma and brain metastases during radiation therapy as a potential predictive marker. First, we developed a multicolor flow cytometry-based assay to monitor the frequency and distribution of T(EMRA) cells in a longitudinal manner. The CMV serostatus and age were considered as influencing factors. We revealed that patients who had a reactivation of CMV have significantly higher amounts of CD8+ T(EMRA) cells. Further, the distribution of the subsets of T(EMRA) cells based on the expression of CD27, CD28, and CD57 is highly dependent on the CMV serostatus. We conclude that the percentage of CD8+ T(EMRA) cells out of all CD8+ T cells has the potential to serve as a biomarker for predicting the risk of CMV reactivation during RT of the brain. Furthermore, this study highlights the importance of taking the CMV serostatus into account when analyzing T(EMRA) cells and their subsets. MDPI 2023-02-04 /pmc/articles/PMC9954596/ /pubmed/36831183 http://dx.doi.org/10.3390/cells12040516 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Scheer, Ilka
Becker, Ina
Schmitter, Charlotte
Semrau, Sabine
Fietkau, Rainer
Gaipl, Udo S.
Frey, Benjamin
Donaubauer, Anna-Jasmina
Prospective Evaluation of CD45RA+/CCR7- Effector Memory T (T(EMRA)) Cell Subsets in Patients with Primary and Secondary Brain Tumors during Radiotherapy of the Brain within the Scope of the Prospective Glio-CMV-01 Clinical Trial
title Prospective Evaluation of CD45RA+/CCR7- Effector Memory T (T(EMRA)) Cell Subsets in Patients with Primary and Secondary Brain Tumors during Radiotherapy of the Brain within the Scope of the Prospective Glio-CMV-01 Clinical Trial
title_full Prospective Evaluation of CD45RA+/CCR7- Effector Memory T (T(EMRA)) Cell Subsets in Patients with Primary and Secondary Brain Tumors during Radiotherapy of the Brain within the Scope of the Prospective Glio-CMV-01 Clinical Trial
title_fullStr Prospective Evaluation of CD45RA+/CCR7- Effector Memory T (T(EMRA)) Cell Subsets in Patients with Primary and Secondary Brain Tumors during Radiotherapy of the Brain within the Scope of the Prospective Glio-CMV-01 Clinical Trial
title_full_unstemmed Prospective Evaluation of CD45RA+/CCR7- Effector Memory T (T(EMRA)) Cell Subsets in Patients with Primary and Secondary Brain Tumors during Radiotherapy of the Brain within the Scope of the Prospective Glio-CMV-01 Clinical Trial
title_short Prospective Evaluation of CD45RA+/CCR7- Effector Memory T (T(EMRA)) Cell Subsets in Patients with Primary and Secondary Brain Tumors during Radiotherapy of the Brain within the Scope of the Prospective Glio-CMV-01 Clinical Trial
title_sort prospective evaluation of cd45ra+/ccr7- effector memory t (t(emra)) cell subsets in patients with primary and secondary brain tumors during radiotherapy of the brain within the scope of the prospective glio-cmv-01 clinical trial
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9954596/
https://www.ncbi.nlm.nih.gov/pubmed/36831183
http://dx.doi.org/10.3390/cells12040516
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