The Genetic and Immunologic Landscape Underlying the Risk of Malignant Progression in Laryngeal Dysplasia

SIMPLE SUMMARY: Our study investigates the alterations of the dysplastic micro-environment and immune system that contribute to the progression from laryngeal dysplasia to invasive cancer. As known, laryngeal dysplasia could represent the initial premalignant lesions for the development of laryngeal...

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Detalles Bibliográficos
Autores principales: Chu, Francesco, Maffini, Fausto, Lepanto, Daniela, Vacirca, Davide, Taormina, Sergio Vincenzo, De Berardinis, Rita, Gandini, Sara, Vignati, Silvano, Ranghiero, Alberto, Rappa, Alessandra, Chiocca, Susanna, Barberis, Massimo, Tagliabue, Marta, Ansarin, Mohssen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9954731/
https://www.ncbi.nlm.nih.gov/pubmed/36831458
http://dx.doi.org/10.3390/cancers15041117
Descripción
Sumario:SIMPLE SUMMARY: Our study investigates the alterations of the dysplastic micro-environment and immune system that contribute to the progression from laryngeal dysplasia to invasive cancer. As known, laryngeal dysplasia could represent the initial premalignant lesions for the development of laryngeal squamous cell cancer. Thus, the possibility to differentiate between progressing (PDy) and non-progressing dysplasia (NPDy) is essential for intensive surveillance programs and cancer prevention. ABSTRACT: (1) Background: The development of laryngeal cancer is a multistep process involving structural alterations of the epithelial mucosa, from dysplasia (LDy) to invasive carcinoma. In this study, we define new biomarkers, prognostic for malignant transformation, in patients affected by LDy. (2) Methods: We used targeted next-generation sequencing and immunohistochemical analysis to define the mutational and immunological landscape of 15 laryngeal dysplasia progressing to invasive cancer (progressing dysplasia), as well as 31 cases of laryngeal dysplasia that did not progress to carcinoma (non-progressing dysplasia). Two pathologists independently analyzed the presence of tumor-infiltrating lymphocytes in LDy pre-embedded paraffin-fixed specimens. The RNA-based next-generation sequencing panel OIRRA was used to evaluate the expression of 395 genes related to immune system activation. (3) Results: High TILs are significantly correlated with a higher risk of malignant transformation. The non-brisk pattern was significantly associated with an 86% reduced risk of malignant progression (OR = 0.16, 95% CI: 0.03–0.5, p = 0.008). TILs showed a highly positive correlation with CCR6, CD83, HLA-DPB1, MX1 and SNAI1, and they were inversely correlated with CD48, CIITA, CXCR4, FCER1G, IL1B, LST1 and TLR8. (4) Conclusions: TILs have a great potential to identify high-risk progression dysplasia and thus to define surveillance protocols and prevention programs.

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