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Assessment of the Accuracy, Usability and Acceptability of a Rapid Test for the Simultaneous Diagnosis of Syphilis and HIV Infection in a Real-Life Scenario in the Amazon Region, Brazil
We field-assessed the accuracy, acceptability, and feasibility of the SD BIOLINE HIV/Syphilis Duo rapid diagnostic test in three groups: pregnant women, female sex workers (FSW), and men who have sex with men (MSM). Venous blood samples collected in the field were compared with the respective gold s...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9955085/ https://www.ncbi.nlm.nih.gov/pubmed/36832298 http://dx.doi.org/10.3390/diagnostics13040810 |
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author | Soares, Daniela Cristina Filho, Luciano Chaves Franco Souza dos Reis, Herald Rodrigues, Yan Corrêa Freitas, Felipe Bonfim de Oliveira Souza, Cintya Damacena, Giseli Nogueira Véras, Nazle Mendonça Collaço Gaspar, Pamela Cristina Benzaken, Adele Schwartz da Felicidade Ribeiro Favacho, Joana Macedo, Olinda Bazzo, Maria Luiza |
author_facet | Soares, Daniela Cristina Filho, Luciano Chaves Franco Souza dos Reis, Herald Rodrigues, Yan Corrêa Freitas, Felipe Bonfim de Oliveira Souza, Cintya Damacena, Giseli Nogueira Véras, Nazle Mendonça Collaço Gaspar, Pamela Cristina Benzaken, Adele Schwartz da Felicidade Ribeiro Favacho, Joana Macedo, Olinda Bazzo, Maria Luiza |
author_sort | Soares, Daniela Cristina |
collection | PubMed |
description | We field-assessed the accuracy, acceptability, and feasibility of the SD BIOLINE HIV/Syphilis Duo rapid diagnostic test in three groups: pregnant women, female sex workers (FSW), and men who have sex with men (MSM). Venous blood samples collected in the field were compared with the respective gold standard methods: SD BIOLINE HIV/Syphilis Duo Treponemal Test versus FTA-abs (Wama brand) treponemal laboratory test for syphilis, and SD BIOLINE HIV/Syphilis Duo Test versus the fourth generation Genscreen Ultra HIV Ag-Ag (Bio-Rad brand) laboratory test for HIV. From a total of 529 participants, 397 (75.1%) were pregnant women, 76 (14.3%) FSW and 56 (10.6%) MSM. Sensitivity and specificity parameters of HIV were 100.0% (95% CI: 82.35–100.0%) and 100.0% (95% CI: 99.28–100.0%), respectively. Sensitivity and specificity parameters found for TP antibody detection were 95.00% (95% CI: 87.69–98.62%) and 100.0% (95% CI: 98.18–100.0%), respectively. The SD BIOLINE HIV/Syphilis Duo Test showed high acceptability among participants (85.87%) and health professionals (85.51%), as well as easy usability by professionals (91.06%). The usability of the SD BIOLINE HIV/Syphilis Duo Test kit would not be a barrier to accessing rapid testing, if the product were incorporated into the list of health service supplies. |
format | Online Article Text |
id | pubmed-9955085 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99550852023-02-25 Assessment of the Accuracy, Usability and Acceptability of a Rapid Test for the Simultaneous Diagnosis of Syphilis and HIV Infection in a Real-Life Scenario in the Amazon Region, Brazil Soares, Daniela Cristina Filho, Luciano Chaves Franco Souza dos Reis, Herald Rodrigues, Yan Corrêa Freitas, Felipe Bonfim de Oliveira Souza, Cintya Damacena, Giseli Nogueira Véras, Nazle Mendonça Collaço Gaspar, Pamela Cristina Benzaken, Adele Schwartz da Felicidade Ribeiro Favacho, Joana Macedo, Olinda Bazzo, Maria Luiza Diagnostics (Basel) Article We field-assessed the accuracy, acceptability, and feasibility of the SD BIOLINE HIV/Syphilis Duo rapid diagnostic test in three groups: pregnant women, female sex workers (FSW), and men who have sex with men (MSM). Venous blood samples collected in the field were compared with the respective gold standard methods: SD BIOLINE HIV/Syphilis Duo Treponemal Test versus FTA-abs (Wama brand) treponemal laboratory test for syphilis, and SD BIOLINE HIV/Syphilis Duo Test versus the fourth generation Genscreen Ultra HIV Ag-Ag (Bio-Rad brand) laboratory test for HIV. From a total of 529 participants, 397 (75.1%) were pregnant women, 76 (14.3%) FSW and 56 (10.6%) MSM. Sensitivity and specificity parameters of HIV were 100.0% (95% CI: 82.35–100.0%) and 100.0% (95% CI: 99.28–100.0%), respectively. Sensitivity and specificity parameters found for TP antibody detection were 95.00% (95% CI: 87.69–98.62%) and 100.0% (95% CI: 98.18–100.0%), respectively. The SD BIOLINE HIV/Syphilis Duo Test showed high acceptability among participants (85.87%) and health professionals (85.51%), as well as easy usability by professionals (91.06%). The usability of the SD BIOLINE HIV/Syphilis Duo Test kit would not be a barrier to accessing rapid testing, if the product were incorporated into the list of health service supplies. MDPI 2023-02-20 /pmc/articles/PMC9955085/ /pubmed/36832298 http://dx.doi.org/10.3390/diagnostics13040810 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Soares, Daniela Cristina Filho, Luciano Chaves Franco Souza dos Reis, Herald Rodrigues, Yan Corrêa Freitas, Felipe Bonfim de Oliveira Souza, Cintya Damacena, Giseli Nogueira Véras, Nazle Mendonça Collaço Gaspar, Pamela Cristina Benzaken, Adele Schwartz da Felicidade Ribeiro Favacho, Joana Macedo, Olinda Bazzo, Maria Luiza Assessment of the Accuracy, Usability and Acceptability of a Rapid Test for the Simultaneous Diagnosis of Syphilis and HIV Infection in a Real-Life Scenario in the Amazon Region, Brazil |
title | Assessment of the Accuracy, Usability and Acceptability of a Rapid Test for the Simultaneous Diagnosis of Syphilis and HIV Infection in a Real-Life Scenario in the Amazon Region, Brazil |
title_full | Assessment of the Accuracy, Usability and Acceptability of a Rapid Test for the Simultaneous Diagnosis of Syphilis and HIV Infection in a Real-Life Scenario in the Amazon Region, Brazil |
title_fullStr | Assessment of the Accuracy, Usability and Acceptability of a Rapid Test for the Simultaneous Diagnosis of Syphilis and HIV Infection in a Real-Life Scenario in the Amazon Region, Brazil |
title_full_unstemmed | Assessment of the Accuracy, Usability and Acceptability of a Rapid Test for the Simultaneous Diagnosis of Syphilis and HIV Infection in a Real-Life Scenario in the Amazon Region, Brazil |
title_short | Assessment of the Accuracy, Usability and Acceptability of a Rapid Test for the Simultaneous Diagnosis of Syphilis and HIV Infection in a Real-Life Scenario in the Amazon Region, Brazil |
title_sort | assessment of the accuracy, usability and acceptability of a rapid test for the simultaneous diagnosis of syphilis and hiv infection in a real-life scenario in the amazon region, brazil |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9955085/ https://www.ncbi.nlm.nih.gov/pubmed/36832298 http://dx.doi.org/10.3390/diagnostics13040810 |
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