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Assessment of the Accuracy, Usability and Acceptability of a Rapid Test for the Simultaneous Diagnosis of Syphilis and HIV Infection in a Real-Life Scenario in the Amazon Region, Brazil

We field-assessed the accuracy, acceptability, and feasibility of the SD BIOLINE HIV/Syphilis Duo rapid diagnostic test in three groups: pregnant women, female sex workers (FSW), and men who have sex with men (MSM). Venous blood samples collected in the field were compared with the respective gold s...

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Autores principales: Soares, Daniela Cristina, Filho, Luciano Chaves Franco, Souza dos Reis, Herald, Rodrigues, Yan Corrêa, Freitas, Felipe Bonfim, de Oliveira Souza, Cintya, Damacena, Giseli Nogueira, Véras, Nazle Mendonça Collaço, Gaspar, Pamela Cristina, Benzaken, Adele Schwartz, da Felicidade Ribeiro Favacho, Joana, Macedo, Olinda, Bazzo, Maria Luiza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9955085/
https://www.ncbi.nlm.nih.gov/pubmed/36832298
http://dx.doi.org/10.3390/diagnostics13040810
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author Soares, Daniela Cristina
Filho, Luciano Chaves Franco
Souza dos Reis, Herald
Rodrigues, Yan Corrêa
Freitas, Felipe Bonfim
de Oliveira Souza, Cintya
Damacena, Giseli Nogueira
Véras, Nazle Mendonça Collaço
Gaspar, Pamela Cristina
Benzaken, Adele Schwartz
da Felicidade Ribeiro Favacho, Joana
Macedo, Olinda
Bazzo, Maria Luiza
author_facet Soares, Daniela Cristina
Filho, Luciano Chaves Franco
Souza dos Reis, Herald
Rodrigues, Yan Corrêa
Freitas, Felipe Bonfim
de Oliveira Souza, Cintya
Damacena, Giseli Nogueira
Véras, Nazle Mendonça Collaço
Gaspar, Pamela Cristina
Benzaken, Adele Schwartz
da Felicidade Ribeiro Favacho, Joana
Macedo, Olinda
Bazzo, Maria Luiza
author_sort Soares, Daniela Cristina
collection PubMed
description We field-assessed the accuracy, acceptability, and feasibility of the SD BIOLINE HIV/Syphilis Duo rapid diagnostic test in three groups: pregnant women, female sex workers (FSW), and men who have sex with men (MSM). Venous blood samples collected in the field were compared with the respective gold standard methods: SD BIOLINE HIV/Syphilis Duo Treponemal Test versus FTA-abs (Wama brand) treponemal laboratory test for syphilis, and SD BIOLINE HIV/Syphilis Duo Test versus the fourth generation Genscreen Ultra HIV Ag-Ag (Bio-Rad brand) laboratory test for HIV. From a total of 529 participants, 397 (75.1%) were pregnant women, 76 (14.3%) FSW and 56 (10.6%) MSM. Sensitivity and specificity parameters of HIV were 100.0% (95% CI: 82.35–100.0%) and 100.0% (95% CI: 99.28–100.0%), respectively. Sensitivity and specificity parameters found for TP antibody detection were 95.00% (95% CI: 87.69–98.62%) and 100.0% (95% CI: 98.18–100.0%), respectively. The SD BIOLINE HIV/Syphilis Duo Test showed high acceptability among participants (85.87%) and health professionals (85.51%), as well as easy usability by professionals (91.06%). The usability of the SD BIOLINE HIV/Syphilis Duo Test kit would not be a barrier to accessing rapid testing, if the product were incorporated into the list of health service supplies.
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spelling pubmed-99550852023-02-25 Assessment of the Accuracy, Usability and Acceptability of a Rapid Test for the Simultaneous Diagnosis of Syphilis and HIV Infection in a Real-Life Scenario in the Amazon Region, Brazil Soares, Daniela Cristina Filho, Luciano Chaves Franco Souza dos Reis, Herald Rodrigues, Yan Corrêa Freitas, Felipe Bonfim de Oliveira Souza, Cintya Damacena, Giseli Nogueira Véras, Nazle Mendonça Collaço Gaspar, Pamela Cristina Benzaken, Adele Schwartz da Felicidade Ribeiro Favacho, Joana Macedo, Olinda Bazzo, Maria Luiza Diagnostics (Basel) Article We field-assessed the accuracy, acceptability, and feasibility of the SD BIOLINE HIV/Syphilis Duo rapid diagnostic test in three groups: pregnant women, female sex workers (FSW), and men who have sex with men (MSM). Venous blood samples collected in the field were compared with the respective gold standard methods: SD BIOLINE HIV/Syphilis Duo Treponemal Test versus FTA-abs (Wama brand) treponemal laboratory test for syphilis, and SD BIOLINE HIV/Syphilis Duo Test versus the fourth generation Genscreen Ultra HIV Ag-Ag (Bio-Rad brand) laboratory test for HIV. From a total of 529 participants, 397 (75.1%) were pregnant women, 76 (14.3%) FSW and 56 (10.6%) MSM. Sensitivity and specificity parameters of HIV were 100.0% (95% CI: 82.35–100.0%) and 100.0% (95% CI: 99.28–100.0%), respectively. Sensitivity and specificity parameters found for TP antibody detection were 95.00% (95% CI: 87.69–98.62%) and 100.0% (95% CI: 98.18–100.0%), respectively. The SD BIOLINE HIV/Syphilis Duo Test showed high acceptability among participants (85.87%) and health professionals (85.51%), as well as easy usability by professionals (91.06%). The usability of the SD BIOLINE HIV/Syphilis Duo Test kit would not be a barrier to accessing rapid testing, if the product were incorporated into the list of health service supplies. MDPI 2023-02-20 /pmc/articles/PMC9955085/ /pubmed/36832298 http://dx.doi.org/10.3390/diagnostics13040810 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Soares, Daniela Cristina
Filho, Luciano Chaves Franco
Souza dos Reis, Herald
Rodrigues, Yan Corrêa
Freitas, Felipe Bonfim
de Oliveira Souza, Cintya
Damacena, Giseli Nogueira
Véras, Nazle Mendonça Collaço
Gaspar, Pamela Cristina
Benzaken, Adele Schwartz
da Felicidade Ribeiro Favacho, Joana
Macedo, Olinda
Bazzo, Maria Luiza
Assessment of the Accuracy, Usability and Acceptability of a Rapid Test for the Simultaneous Diagnosis of Syphilis and HIV Infection in a Real-Life Scenario in the Amazon Region, Brazil
title Assessment of the Accuracy, Usability and Acceptability of a Rapid Test for the Simultaneous Diagnosis of Syphilis and HIV Infection in a Real-Life Scenario in the Amazon Region, Brazil
title_full Assessment of the Accuracy, Usability and Acceptability of a Rapid Test for the Simultaneous Diagnosis of Syphilis and HIV Infection in a Real-Life Scenario in the Amazon Region, Brazil
title_fullStr Assessment of the Accuracy, Usability and Acceptability of a Rapid Test for the Simultaneous Diagnosis of Syphilis and HIV Infection in a Real-Life Scenario in the Amazon Region, Brazil
title_full_unstemmed Assessment of the Accuracy, Usability and Acceptability of a Rapid Test for the Simultaneous Diagnosis of Syphilis and HIV Infection in a Real-Life Scenario in the Amazon Region, Brazil
title_short Assessment of the Accuracy, Usability and Acceptability of a Rapid Test for the Simultaneous Diagnosis of Syphilis and HIV Infection in a Real-Life Scenario in the Amazon Region, Brazil
title_sort assessment of the accuracy, usability and acceptability of a rapid test for the simultaneous diagnosis of syphilis and hiv infection in a real-life scenario in the amazon region, brazil
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9955085/
https://www.ncbi.nlm.nih.gov/pubmed/36832298
http://dx.doi.org/10.3390/diagnostics13040810
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