Cargando…

First Insight into Diversity of Minisatellite Loci in Mycobacterium bovis/M. caprae in Bulgaria

The aim of this study was to assess the diversity of minisatellite VNTR loci in Mycobacterium bovis/M. caprae isolates in Bulgaria and view their position within global M. bovis diversity. Forty-three M. bovis/M. caprae isolates from cattle in different farms in Bulgaria were collected in 2015–2021...

Descripción completa

Detalles Bibliográficos
Autores principales: Terentieva, Daria, Savova-Lalkovska, Tanya, Dimitrova, Albena, Bonovska, Magdalena, Mokrousov, Igor, Valcheva, Violeta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9955489/
https://www.ncbi.nlm.nih.gov/pubmed/36832259
http://dx.doi.org/10.3390/diagnostics13040771
_version_ 1784894358599761920
author Terentieva, Daria
Savova-Lalkovska, Tanya
Dimitrova, Albena
Bonovska, Magdalena
Mokrousov, Igor
Valcheva, Violeta
author_facet Terentieva, Daria
Savova-Lalkovska, Tanya
Dimitrova, Albena
Bonovska, Magdalena
Mokrousov, Igor
Valcheva, Violeta
author_sort Terentieva, Daria
collection PubMed
description The aim of this study was to assess the diversity of minisatellite VNTR loci in Mycobacterium bovis/M. caprae isolates in Bulgaria and view their position within global M. bovis diversity. Forty-three M. bovis/M. caprae isolates from cattle in different farms in Bulgaria were collected in 2015–2021 and typed in 13 VNTR loci. The M. bovis and M. caprae branches were clearly separated on the VNTR phylogenetic tree. The larger and more geographically dispersed M. caprae group was more diverse than M. bovis group was (HGI 0.67 vs. 0.60). Overall, six clusters were identified (from 2 to 19 isolates) and nine orphans (all loci-based HGI 0.79). Locus QUB3232 was the most discriminatory one (HGI 0.64). MIRU4 and MIRU40 were monomorphic, and MIRU26 was almost monomorphic. Four loci (ETRA, ETRB, Mtub21, and MIRU16) discriminated only between M. bovis and M. caprae. The comparison with published VNTR datasets from 11 countries showed both overall heterogeneity between the settings and predominantly local evolution of the clonal complexes. To conclude, six loci may be recommended for primary genotyping of M. bovis/M. caprae isolates in Bulgaria: ETRC, QUB11b, QUB11a, QUB26, QUB3232, and MIRU10 (HGI 0.77). VNTR typing based on a limited number of loci appears to be useful for primary bTB surveillance.
format Online
Article
Text
id pubmed-9955489
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-99554892023-02-25 First Insight into Diversity of Minisatellite Loci in Mycobacterium bovis/M. caprae in Bulgaria Terentieva, Daria Savova-Lalkovska, Tanya Dimitrova, Albena Bonovska, Magdalena Mokrousov, Igor Valcheva, Violeta Diagnostics (Basel) Article The aim of this study was to assess the diversity of minisatellite VNTR loci in Mycobacterium bovis/M. caprae isolates in Bulgaria and view their position within global M. bovis diversity. Forty-three M. bovis/M. caprae isolates from cattle in different farms in Bulgaria were collected in 2015–2021 and typed in 13 VNTR loci. The M. bovis and M. caprae branches were clearly separated on the VNTR phylogenetic tree. The larger and more geographically dispersed M. caprae group was more diverse than M. bovis group was (HGI 0.67 vs. 0.60). Overall, six clusters were identified (from 2 to 19 isolates) and nine orphans (all loci-based HGI 0.79). Locus QUB3232 was the most discriminatory one (HGI 0.64). MIRU4 and MIRU40 were monomorphic, and MIRU26 was almost monomorphic. Four loci (ETRA, ETRB, Mtub21, and MIRU16) discriminated only between M. bovis and M. caprae. The comparison with published VNTR datasets from 11 countries showed both overall heterogeneity between the settings and predominantly local evolution of the clonal complexes. To conclude, six loci may be recommended for primary genotyping of M. bovis/M. caprae isolates in Bulgaria: ETRC, QUB11b, QUB11a, QUB26, QUB3232, and MIRU10 (HGI 0.77). VNTR typing based on a limited number of loci appears to be useful for primary bTB surveillance. MDPI 2023-02-17 /pmc/articles/PMC9955489/ /pubmed/36832259 http://dx.doi.org/10.3390/diagnostics13040771 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Terentieva, Daria
Savova-Lalkovska, Tanya
Dimitrova, Albena
Bonovska, Magdalena
Mokrousov, Igor
Valcheva, Violeta
First Insight into Diversity of Minisatellite Loci in Mycobacterium bovis/M. caprae in Bulgaria
title First Insight into Diversity of Minisatellite Loci in Mycobacterium bovis/M. caprae in Bulgaria
title_full First Insight into Diversity of Minisatellite Loci in Mycobacterium bovis/M. caprae in Bulgaria
title_fullStr First Insight into Diversity of Minisatellite Loci in Mycobacterium bovis/M. caprae in Bulgaria
title_full_unstemmed First Insight into Diversity of Minisatellite Loci in Mycobacterium bovis/M. caprae in Bulgaria
title_short First Insight into Diversity of Minisatellite Loci in Mycobacterium bovis/M. caprae in Bulgaria
title_sort first insight into diversity of minisatellite loci in mycobacterium bovis/m. caprae in bulgaria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9955489/
https://www.ncbi.nlm.nih.gov/pubmed/36832259
http://dx.doi.org/10.3390/diagnostics13040771
work_keys_str_mv AT terentievadaria firstinsightintodiversityofminisatellitelociinmycobacteriumbovismcapraeinbulgaria
AT savovalalkovskatanya firstinsightintodiversityofminisatellitelociinmycobacteriumbovismcapraeinbulgaria
AT dimitrovaalbena firstinsightintodiversityofminisatellitelociinmycobacteriumbovismcapraeinbulgaria
AT bonovskamagdalena firstinsightintodiversityofminisatellitelociinmycobacteriumbovismcapraeinbulgaria
AT mokrousovigor firstinsightintodiversityofminisatellitelociinmycobacteriumbovismcapraeinbulgaria
AT valchevavioleta firstinsightintodiversityofminisatellitelociinmycobacteriumbovismcapraeinbulgaria