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Hypobaric hypoxia induced renal injury in rats: Prophylactic amelioration by quercetin supplementation
The present study aims at assessing the effect of hypobaric hypoxia induced renal damage and associated renal functions in male SD rats. Further, this study was extended to explore the protective efficacy of quercetin in ameliorating the functional impairment in kidneys of rats under hypobaric hypox...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9955615/ https://www.ncbi.nlm.nih.gov/pubmed/36827356 http://dx.doi.org/10.1371/journal.pone.0279304 |
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author | Rathi, Vaishnavi Tiwari, Isha Kulshreshtha, Ritu S. K. Sagi, Sarada |
author_facet | Rathi, Vaishnavi Tiwari, Isha Kulshreshtha, Ritu S. K. Sagi, Sarada |
author_sort | Rathi, Vaishnavi |
collection | PubMed |
description | The present study aims at assessing the effect of hypobaric hypoxia induced renal damage and associated renal functions in male SD rats. Further, this study was extended to explore the protective efficacy of quercetin in ameliorating the functional impairment in kidneys of rats under hypobaric hypoxia. Rats were exposed to 7620m (25000 ft.) at 25°C ±2 in a simulated hypobaric hypoxia chamber for different time durations (0h,1h, 3h, 6h, 12h, 24h and 48h) in order to optimize the time at which maximum renal damage would occur. The rats were exposed to hypoxia for 12h duration was considered as the optimum time, due to significant increase in oxidative stress (ROS, MDA) and renal metabolites (creatinine, BUN and uric acid) with remarkable reduction (p<0.001) in antioxidants (GSH) in plasma, as compared to other tested durations. Moreover, these findings were in support with the histopathology analysis of renal tissues. For optimum quercetin dose selection, the rats were administered with different doses of quercetin (25mg, 50mg, 100mg and 200mg/Kg BW) for 12h at 7620 m, 25°C ±2, 1h prior to hypoxia exposure. Quercetin 50mg/kg BW was considered as the optimum dose at which significant (p<0.001) reduction in oxidative stress levels followed by reduction in creatinine and BUN levels were obtained in plasma of the rats compared to hypoxia control rats. Quercetin prophylaxis (50mg/kg BW) stabilized the HIF-1α protein expression followed by reduced VEGF protein expression along with reduced levels of LDH (p<0.001) in the kidneys of rats compared to hypoxia control. Histopathological observations further substantiated these findings in reducing the renal tissue injury. The study findings revealed that, quercetin prophylaxis abrogates the possibility of hypobaric hypoxia induced renal injury by reducing the oxidative stress in rats. |
format | Online Article Text |
id | pubmed-9955615 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-99556152023-02-25 Hypobaric hypoxia induced renal injury in rats: Prophylactic amelioration by quercetin supplementation Rathi, Vaishnavi Tiwari, Isha Kulshreshtha, Ritu S. K. Sagi, Sarada PLoS One Research Article The present study aims at assessing the effect of hypobaric hypoxia induced renal damage and associated renal functions in male SD rats. Further, this study was extended to explore the protective efficacy of quercetin in ameliorating the functional impairment in kidneys of rats under hypobaric hypoxia. Rats were exposed to 7620m (25000 ft.) at 25°C ±2 in a simulated hypobaric hypoxia chamber for different time durations (0h,1h, 3h, 6h, 12h, 24h and 48h) in order to optimize the time at which maximum renal damage would occur. The rats were exposed to hypoxia for 12h duration was considered as the optimum time, due to significant increase in oxidative stress (ROS, MDA) and renal metabolites (creatinine, BUN and uric acid) with remarkable reduction (p<0.001) in antioxidants (GSH) in plasma, as compared to other tested durations. Moreover, these findings were in support with the histopathology analysis of renal tissues. For optimum quercetin dose selection, the rats were administered with different doses of quercetin (25mg, 50mg, 100mg and 200mg/Kg BW) for 12h at 7620 m, 25°C ±2, 1h prior to hypoxia exposure. Quercetin 50mg/kg BW was considered as the optimum dose at which significant (p<0.001) reduction in oxidative stress levels followed by reduction in creatinine and BUN levels were obtained in plasma of the rats compared to hypoxia control rats. Quercetin prophylaxis (50mg/kg BW) stabilized the HIF-1α protein expression followed by reduced VEGF protein expression along with reduced levels of LDH (p<0.001) in the kidneys of rats compared to hypoxia control. Histopathological observations further substantiated these findings in reducing the renal tissue injury. The study findings revealed that, quercetin prophylaxis abrogates the possibility of hypobaric hypoxia induced renal injury by reducing the oxidative stress in rats. Public Library of Science 2023-02-24 /pmc/articles/PMC9955615/ /pubmed/36827356 http://dx.doi.org/10.1371/journal.pone.0279304 Text en © 2023 Rathi et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Rathi, Vaishnavi Tiwari, Isha Kulshreshtha, Ritu S. K. Sagi, Sarada Hypobaric hypoxia induced renal injury in rats: Prophylactic amelioration by quercetin supplementation |
title | Hypobaric hypoxia induced renal injury in rats: Prophylactic amelioration by quercetin supplementation |
title_full | Hypobaric hypoxia induced renal injury in rats: Prophylactic amelioration by quercetin supplementation |
title_fullStr | Hypobaric hypoxia induced renal injury in rats: Prophylactic amelioration by quercetin supplementation |
title_full_unstemmed | Hypobaric hypoxia induced renal injury in rats: Prophylactic amelioration by quercetin supplementation |
title_short | Hypobaric hypoxia induced renal injury in rats: Prophylactic amelioration by quercetin supplementation |
title_sort | hypobaric hypoxia induced renal injury in rats: prophylactic amelioration by quercetin supplementation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9955615/ https://www.ncbi.nlm.nih.gov/pubmed/36827356 http://dx.doi.org/10.1371/journal.pone.0279304 |
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