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Proteomics and Molecular Docking Analyses Reveal the Bio-Chemical and Molecular Mechanism Underlying the Hypolipidemic Activity of Nano-Liposomal Bioactive Peptides in 3T3-L1 Adipocytes

Obesity is a global health concern. Physical activities and eating nutrient-rich functional foods can prevent obesity. In this study, nano-liposomal encapsulated bioactive peptides (BPs) were developed to reduce cellular lipids. The peptide sequence NH(2)-PCGVPMLTVAEQAQ-CO(2)H was chemically synthes...

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Autores principales: Krobthong, Sucheewin, Yingchutrakul, Yodying, Wongtrakoongate, Patompon, Chuntakaruk, Hathaichanok, Rungrotmongkol, Thanyada, Chaichana, Chartchai, Mahatnirunkul, Thanisorn, Chomtong, Thitikorn, Choowongkomon, Kiattawee, Aonbangkhen, Chanat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9956075/
https://www.ncbi.nlm.nih.gov/pubmed/36832854
http://dx.doi.org/10.3390/foods12040780
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author Krobthong, Sucheewin
Yingchutrakul, Yodying
Wongtrakoongate, Patompon
Chuntakaruk, Hathaichanok
Rungrotmongkol, Thanyada
Chaichana, Chartchai
Mahatnirunkul, Thanisorn
Chomtong, Thitikorn
Choowongkomon, Kiattawee
Aonbangkhen, Chanat
author_facet Krobthong, Sucheewin
Yingchutrakul, Yodying
Wongtrakoongate, Patompon
Chuntakaruk, Hathaichanok
Rungrotmongkol, Thanyada
Chaichana, Chartchai
Mahatnirunkul, Thanisorn
Chomtong, Thitikorn
Choowongkomon, Kiattawee
Aonbangkhen, Chanat
author_sort Krobthong, Sucheewin
collection PubMed
description Obesity is a global health concern. Physical activities and eating nutrient-rich functional foods can prevent obesity. In this study, nano-liposomal encapsulated bioactive peptides (BPs) were developed to reduce cellular lipids. The peptide sequence NH(2)-PCGVPMLTVAEQAQ-CO(2)H was chemically synthesized. The limited membrane permeability of the BPs was improved by encapsulating the BPs with a nano-liposomal carrier, which was produced by thin-layer formation. The nano-liposomal BPs had a diameter of ~157 nm and were monodispersed in solution. The encapsulation capacity was 61.2 ± 3.2%. The nano-liposomal BPs had no significant cytotoxicity on the tested cells, keratinocytes, fibroblasts, and adipocytes. The in vitro hypolipidemic activity significantly promoted the breakdown of triglycerides (TGs). Lipid droplet staining was correlated with TG content. Proteomics analysis identified 2418 differentially expressed proteins. The nano-liposomal BPs affected various biochemical pathways beyond lipolysis. The nano-liposomal BP treatment decreased the fatty acid synthase expression by 17.41 ± 1.17%. HDOCK revealed that the BPs inhibited fatty acid synthase (FAS) at the thioesterase domain. The HDOCK score of the BPs was lower than that of orlistat, a known obesity drug, indicating stronger binding. Proteomics and molecular docking analyses confirmed that the nano-liposomal BPs were suitable for use in functional foods to prevent obesity.
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spelling pubmed-99560752023-02-25 Proteomics and Molecular Docking Analyses Reveal the Bio-Chemical and Molecular Mechanism Underlying the Hypolipidemic Activity of Nano-Liposomal Bioactive Peptides in 3T3-L1 Adipocytes Krobthong, Sucheewin Yingchutrakul, Yodying Wongtrakoongate, Patompon Chuntakaruk, Hathaichanok Rungrotmongkol, Thanyada Chaichana, Chartchai Mahatnirunkul, Thanisorn Chomtong, Thitikorn Choowongkomon, Kiattawee Aonbangkhen, Chanat Foods Article Obesity is a global health concern. Physical activities and eating nutrient-rich functional foods can prevent obesity. In this study, nano-liposomal encapsulated bioactive peptides (BPs) were developed to reduce cellular lipids. The peptide sequence NH(2)-PCGVPMLTVAEQAQ-CO(2)H was chemically synthesized. The limited membrane permeability of the BPs was improved by encapsulating the BPs with a nano-liposomal carrier, which was produced by thin-layer formation. The nano-liposomal BPs had a diameter of ~157 nm and were monodispersed in solution. The encapsulation capacity was 61.2 ± 3.2%. The nano-liposomal BPs had no significant cytotoxicity on the tested cells, keratinocytes, fibroblasts, and adipocytes. The in vitro hypolipidemic activity significantly promoted the breakdown of triglycerides (TGs). Lipid droplet staining was correlated with TG content. Proteomics analysis identified 2418 differentially expressed proteins. The nano-liposomal BPs affected various biochemical pathways beyond lipolysis. The nano-liposomal BP treatment decreased the fatty acid synthase expression by 17.41 ± 1.17%. HDOCK revealed that the BPs inhibited fatty acid synthase (FAS) at the thioesterase domain. The HDOCK score of the BPs was lower than that of orlistat, a known obesity drug, indicating stronger binding. Proteomics and molecular docking analyses confirmed that the nano-liposomal BPs were suitable for use in functional foods to prevent obesity. MDPI 2023-02-10 /pmc/articles/PMC9956075/ /pubmed/36832854 http://dx.doi.org/10.3390/foods12040780 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Krobthong, Sucheewin
Yingchutrakul, Yodying
Wongtrakoongate, Patompon
Chuntakaruk, Hathaichanok
Rungrotmongkol, Thanyada
Chaichana, Chartchai
Mahatnirunkul, Thanisorn
Chomtong, Thitikorn
Choowongkomon, Kiattawee
Aonbangkhen, Chanat
Proteomics and Molecular Docking Analyses Reveal the Bio-Chemical and Molecular Mechanism Underlying the Hypolipidemic Activity of Nano-Liposomal Bioactive Peptides in 3T3-L1 Adipocytes
title Proteomics and Molecular Docking Analyses Reveal the Bio-Chemical and Molecular Mechanism Underlying the Hypolipidemic Activity of Nano-Liposomal Bioactive Peptides in 3T3-L1 Adipocytes
title_full Proteomics and Molecular Docking Analyses Reveal the Bio-Chemical and Molecular Mechanism Underlying the Hypolipidemic Activity of Nano-Liposomal Bioactive Peptides in 3T3-L1 Adipocytes
title_fullStr Proteomics and Molecular Docking Analyses Reveal the Bio-Chemical and Molecular Mechanism Underlying the Hypolipidemic Activity of Nano-Liposomal Bioactive Peptides in 3T3-L1 Adipocytes
title_full_unstemmed Proteomics and Molecular Docking Analyses Reveal the Bio-Chemical and Molecular Mechanism Underlying the Hypolipidemic Activity of Nano-Liposomal Bioactive Peptides in 3T3-L1 Adipocytes
title_short Proteomics and Molecular Docking Analyses Reveal the Bio-Chemical and Molecular Mechanism Underlying the Hypolipidemic Activity of Nano-Liposomal Bioactive Peptides in 3T3-L1 Adipocytes
title_sort proteomics and molecular docking analyses reveal the bio-chemical and molecular mechanism underlying the hypolipidemic activity of nano-liposomal bioactive peptides in 3t3-l1 adipocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9956075/
https://www.ncbi.nlm.nih.gov/pubmed/36832854
http://dx.doi.org/10.3390/foods12040780
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