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DNA Double-Strand Break-Related Competitive Endogenous RNA Network of Noncoding RNA in Bovine Cumulus Cells

(1) Background: DNA double strand breaks (DSBs) are the most serious form of DNA damage that affects oocyte maturation and the physiological state of follicles and ovaries. Non-coding RNAs (ncRNAs) play a crucial role in DNA damage and repair. This study aims to analyze and establish the network of...

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Autores principales: Liu, Jian-Bo, Zhang, Jia-Bao, Yan, Xiang-Min, Xie, Peng-Gui, Fu, Yao, Fu, Xu-Huang, Sun, Xu-Lei, Han, Dong-Xu, Li, Sheng-Peng, Zheng, Yi, Gao, Yan, Kim, Nam-Hyung, Yuan, Bao, Jiang, Hao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9956238/
https://www.ncbi.nlm.nih.gov/pubmed/36833217
http://dx.doi.org/10.3390/genes14020290
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author Liu, Jian-Bo
Zhang, Jia-Bao
Yan, Xiang-Min
Xie, Peng-Gui
Fu, Yao
Fu, Xu-Huang
Sun, Xu-Lei
Han, Dong-Xu
Li, Sheng-Peng
Zheng, Yi
Gao, Yan
Kim, Nam-Hyung
Yuan, Bao
Jiang, Hao
author_facet Liu, Jian-Bo
Zhang, Jia-Bao
Yan, Xiang-Min
Xie, Peng-Gui
Fu, Yao
Fu, Xu-Huang
Sun, Xu-Lei
Han, Dong-Xu
Li, Sheng-Peng
Zheng, Yi
Gao, Yan
Kim, Nam-Hyung
Yuan, Bao
Jiang, Hao
author_sort Liu, Jian-Bo
collection PubMed
description (1) Background: DNA double strand breaks (DSBs) are the most serious form of DNA damage that affects oocyte maturation and the physiological state of follicles and ovaries. Non-coding RNAs (ncRNAs) play a crucial role in DNA damage and repair. This study aims to analyze and establish the network of ncRNAs when DSB occurs and provide new ideas for next research on the mechanism of cumulus DSB. (2) Methods: Bovine cumulus cells (CCs) were treated with bleomycin (BLM) to construct a DSB model. We detected the changes of the cell cycle, cell viability, and apoptosis to determine the effect of DSBs on cell biology, and further evaluated the relationship between the transcriptome and competitive endogenous RNA (ceRNA) network and DSBs. (3) Results: BLM increased γH2AX positivity in CCs, disrupted the G1/S phase, and decreased cell viability. Totals of 848 mRNAs, 75 long noncoding RNAs (lncRNAs), 68 circular RNAs (circRNAs), and 71 microRNAs (miRNAs) in 78 groups of lncRNA–miRNA–mRNA regulatory networks, 275 groups of circRNA–miRNA–mRNA regulatory networks, and five groups of lncRNA/circRNA–miRNA–mRNA co-expression regulatory networks were related to DSBs. Most differentially expressed ncRNAs were annotated to cell cycle, p53, PI3K-AKT, and WNT signaling pathways. (4) Conclusions: The ceRNA network helps to understand the effects of DNA DSBs activation and remission on the biological function of CCs.
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spelling pubmed-99562382023-02-25 DNA Double-Strand Break-Related Competitive Endogenous RNA Network of Noncoding RNA in Bovine Cumulus Cells Liu, Jian-Bo Zhang, Jia-Bao Yan, Xiang-Min Xie, Peng-Gui Fu, Yao Fu, Xu-Huang Sun, Xu-Lei Han, Dong-Xu Li, Sheng-Peng Zheng, Yi Gao, Yan Kim, Nam-Hyung Yuan, Bao Jiang, Hao Genes (Basel) Article (1) Background: DNA double strand breaks (DSBs) are the most serious form of DNA damage that affects oocyte maturation and the physiological state of follicles and ovaries. Non-coding RNAs (ncRNAs) play a crucial role in DNA damage and repair. This study aims to analyze and establish the network of ncRNAs when DSB occurs and provide new ideas for next research on the mechanism of cumulus DSB. (2) Methods: Bovine cumulus cells (CCs) were treated with bleomycin (BLM) to construct a DSB model. We detected the changes of the cell cycle, cell viability, and apoptosis to determine the effect of DSBs on cell biology, and further evaluated the relationship between the transcriptome and competitive endogenous RNA (ceRNA) network and DSBs. (3) Results: BLM increased γH2AX positivity in CCs, disrupted the G1/S phase, and decreased cell viability. Totals of 848 mRNAs, 75 long noncoding RNAs (lncRNAs), 68 circular RNAs (circRNAs), and 71 microRNAs (miRNAs) in 78 groups of lncRNA–miRNA–mRNA regulatory networks, 275 groups of circRNA–miRNA–mRNA regulatory networks, and five groups of lncRNA/circRNA–miRNA–mRNA co-expression regulatory networks were related to DSBs. Most differentially expressed ncRNAs were annotated to cell cycle, p53, PI3K-AKT, and WNT signaling pathways. (4) Conclusions: The ceRNA network helps to understand the effects of DNA DSBs activation and remission on the biological function of CCs. MDPI 2023-01-22 /pmc/articles/PMC9956238/ /pubmed/36833217 http://dx.doi.org/10.3390/genes14020290 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Jian-Bo
Zhang, Jia-Bao
Yan, Xiang-Min
Xie, Peng-Gui
Fu, Yao
Fu, Xu-Huang
Sun, Xu-Lei
Han, Dong-Xu
Li, Sheng-Peng
Zheng, Yi
Gao, Yan
Kim, Nam-Hyung
Yuan, Bao
Jiang, Hao
DNA Double-Strand Break-Related Competitive Endogenous RNA Network of Noncoding RNA in Bovine Cumulus Cells
title DNA Double-Strand Break-Related Competitive Endogenous RNA Network of Noncoding RNA in Bovine Cumulus Cells
title_full DNA Double-Strand Break-Related Competitive Endogenous RNA Network of Noncoding RNA in Bovine Cumulus Cells
title_fullStr DNA Double-Strand Break-Related Competitive Endogenous RNA Network of Noncoding RNA in Bovine Cumulus Cells
title_full_unstemmed DNA Double-Strand Break-Related Competitive Endogenous RNA Network of Noncoding RNA in Bovine Cumulus Cells
title_short DNA Double-Strand Break-Related Competitive Endogenous RNA Network of Noncoding RNA in Bovine Cumulus Cells
title_sort dna double-strand break-related competitive endogenous rna network of noncoding rna in bovine cumulus cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9956238/
https://www.ncbi.nlm.nih.gov/pubmed/36833217
http://dx.doi.org/10.3390/genes14020290
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