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Ficus carica (Linn.) Leaf and Bud Extracts and Their Combination Attenuates Type-1 Diabetes and Its Complications via the Inhibition of Oxidative Stress

The current work was designed to evaluate the antioxidant activity and antidiabetic effect of Ficus carica L. extracts. For that, the leaves and buds of Ficus carica L. were analyzed to determine their polyphenolic and flavonoid contents and antioxidant activity. Diabetes was induced by a single dos...

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Autores principales: El Ghouizi, Asmae, Ousaaid, Driss, Laaroussi, Hassan, Bakour, Meryem, Aboulghazi, Abderrazak, Soutien, Rose Strutch, Hano, Christophe, Lyoussi, Badiaa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9956282/
https://www.ncbi.nlm.nih.gov/pubmed/36832834
http://dx.doi.org/10.3390/foods12040759
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author El Ghouizi, Asmae
Ousaaid, Driss
Laaroussi, Hassan
Bakour, Meryem
Aboulghazi, Abderrazak
Soutien, Rose Strutch
Hano, Christophe
Lyoussi, Badiaa
author_facet El Ghouizi, Asmae
Ousaaid, Driss
Laaroussi, Hassan
Bakour, Meryem
Aboulghazi, Abderrazak
Soutien, Rose Strutch
Hano, Christophe
Lyoussi, Badiaa
author_sort El Ghouizi, Asmae
collection PubMed
description The current work was designed to evaluate the antioxidant activity and antidiabetic effect of Ficus carica L. extracts. For that, the leaves and buds of Ficus carica L. were analyzed to determine their polyphenolic and flavonoid contents and antioxidant activity. Diabetes was induced by a single dose of alloxan monohydrate (65 mg/kg body weight), then diabetic rats were treated with a dose of 200 mg/kg body weight of the methanolic extracts of Ficus carica leaves or buds or their combination for 30 days. Throughout the experiment, blood sugar and body weight were measured every 5 and 7 days respectively. At the end of the experiment, serum and urine were collected for analysis of alanine aminotransferase, aspartate aminotransferase, total cholesterol, triglycerides, creatinine, uric acid, urea, proteins, sodium, potassium, and chloride. Pancreas, liver, and kidney were removed to estimate catalase, glutathione peroxidase, and glutathione activities; lipid peroxidation products were also determined. The results obtained revealed that alloxan has induced hyperglycemia, increased liver and renal biomarkers levels, reduced antioxidative enzymes, and induced lipid peroxidation. However, the treatment with Ficus carica leaf and bud extracts, especially their combination, has attenuated all pharmacological perturbations induced by alloxan.
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spelling pubmed-99562822023-02-25 Ficus carica (Linn.) Leaf and Bud Extracts and Their Combination Attenuates Type-1 Diabetes and Its Complications via the Inhibition of Oxidative Stress El Ghouizi, Asmae Ousaaid, Driss Laaroussi, Hassan Bakour, Meryem Aboulghazi, Abderrazak Soutien, Rose Strutch Hano, Christophe Lyoussi, Badiaa Foods Article The current work was designed to evaluate the antioxidant activity and antidiabetic effect of Ficus carica L. extracts. For that, the leaves and buds of Ficus carica L. were analyzed to determine their polyphenolic and flavonoid contents and antioxidant activity. Diabetes was induced by a single dose of alloxan monohydrate (65 mg/kg body weight), then diabetic rats were treated with a dose of 200 mg/kg body weight of the methanolic extracts of Ficus carica leaves or buds or their combination for 30 days. Throughout the experiment, blood sugar and body weight were measured every 5 and 7 days respectively. At the end of the experiment, serum and urine were collected for analysis of alanine aminotransferase, aspartate aminotransferase, total cholesterol, triglycerides, creatinine, uric acid, urea, proteins, sodium, potassium, and chloride. Pancreas, liver, and kidney were removed to estimate catalase, glutathione peroxidase, and glutathione activities; lipid peroxidation products were also determined. The results obtained revealed that alloxan has induced hyperglycemia, increased liver and renal biomarkers levels, reduced antioxidative enzymes, and induced lipid peroxidation. However, the treatment with Ficus carica leaf and bud extracts, especially their combination, has attenuated all pharmacological perturbations induced by alloxan. MDPI 2023-02-09 /pmc/articles/PMC9956282/ /pubmed/36832834 http://dx.doi.org/10.3390/foods12040759 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
El Ghouizi, Asmae
Ousaaid, Driss
Laaroussi, Hassan
Bakour, Meryem
Aboulghazi, Abderrazak
Soutien, Rose Strutch
Hano, Christophe
Lyoussi, Badiaa
Ficus carica (Linn.) Leaf and Bud Extracts and Their Combination Attenuates Type-1 Diabetes and Its Complications via the Inhibition of Oxidative Stress
title Ficus carica (Linn.) Leaf and Bud Extracts and Their Combination Attenuates Type-1 Diabetes and Its Complications via the Inhibition of Oxidative Stress
title_full Ficus carica (Linn.) Leaf and Bud Extracts and Their Combination Attenuates Type-1 Diabetes and Its Complications via the Inhibition of Oxidative Stress
title_fullStr Ficus carica (Linn.) Leaf and Bud Extracts and Their Combination Attenuates Type-1 Diabetes and Its Complications via the Inhibition of Oxidative Stress
title_full_unstemmed Ficus carica (Linn.) Leaf and Bud Extracts and Their Combination Attenuates Type-1 Diabetes and Its Complications via the Inhibition of Oxidative Stress
title_short Ficus carica (Linn.) Leaf and Bud Extracts and Their Combination Attenuates Type-1 Diabetes and Its Complications via the Inhibition of Oxidative Stress
title_sort ficus carica (linn.) leaf and bud extracts and their combination attenuates type-1 diabetes and its complications via the inhibition of oxidative stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9956282/
https://www.ncbi.nlm.nih.gov/pubmed/36832834
http://dx.doi.org/10.3390/foods12040759
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