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MCD Inhibits Lipid Deposition in Goat Intramuscular Preadipocytes

Malonyl-CoA decarboxylase (MCD) is a major regulator of fatty acid oxidation catalyzing the decarboxylation of malonyl coenzyme A (malonyl-CoA). Although its involvement in human diseases has been well studied, its role in intramuscular fat (IMF) deposition remains unknown. In this present study, 17...

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Autores principales: Yang, Changheng, Li, Qi, Lin, Yaqiu, Wang, Yong, Shi, Hengbo, Huang, Lian, Zhao, Wangsheng, Xiang, Hua, Zhu, Jiangjiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9956415/
https://www.ncbi.nlm.nih.gov/pubmed/36833367
http://dx.doi.org/10.3390/genes14020440
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author Yang, Changheng
Li, Qi
Lin, Yaqiu
Wang, Yong
Shi, Hengbo
Huang, Lian
Zhao, Wangsheng
Xiang, Hua
Zhu, Jiangjiang
author_facet Yang, Changheng
Li, Qi
Lin, Yaqiu
Wang, Yong
Shi, Hengbo
Huang, Lian
Zhao, Wangsheng
Xiang, Hua
Zhu, Jiangjiang
author_sort Yang, Changheng
collection PubMed
description Malonyl-CoA decarboxylase (MCD) is a major regulator of fatty acid oxidation catalyzing the decarboxylation of malonyl coenzyme A (malonyl-CoA). Although its involvement in human diseases has been well studied, its role in intramuscular fat (IMF) deposition remains unknown. In this present study, 1726 bp of MCD cDNA was cloned (OM937122) from goat liver, including 5′UTR of 27 bp, 3′UTR of 199 bp, and CDS of 1500 bp, encoding 499 amino acids. In this present study, although the overexpression of MCD increased the mRNA expression of FASN and DGAT2, the expression of ATGL and ACOX1 was also activated significantly and resulted in a decrease in cellular lipid deposition in goat intramuscular preadipocytes. Meanwhile, the silencing of MCD increased the cellular lipid deposition and was accompanied by the expression activation of DGAT2 and the expression suppression of ATGL and HSL, despite the expression suppression of genes related to fatty acid synthesis, including ACC and FASN. However, the expression of DGAT1 was not affected significantly (p > 0.05) by the expression alteration of MCD in this present study. Furthermore, 2025 bp of MCD promoter was obtained and predicted to be regulated by C/EBPα, SP1, SREBP1, and PPARG. In summary, although different pathways may respond to the expression alteration of MCD, the expression of MCD was negatively correlated with the cellular lipid deposition in goat intramuscular preadipocytes. These data may be beneficial for enhancing our understanding of the regulation of IMF deposition in goats.
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spelling pubmed-99564152023-02-25 MCD Inhibits Lipid Deposition in Goat Intramuscular Preadipocytes Yang, Changheng Li, Qi Lin, Yaqiu Wang, Yong Shi, Hengbo Huang, Lian Zhao, Wangsheng Xiang, Hua Zhu, Jiangjiang Genes (Basel) Article Malonyl-CoA decarboxylase (MCD) is a major regulator of fatty acid oxidation catalyzing the decarboxylation of malonyl coenzyme A (malonyl-CoA). Although its involvement in human diseases has been well studied, its role in intramuscular fat (IMF) deposition remains unknown. In this present study, 1726 bp of MCD cDNA was cloned (OM937122) from goat liver, including 5′UTR of 27 bp, 3′UTR of 199 bp, and CDS of 1500 bp, encoding 499 amino acids. In this present study, although the overexpression of MCD increased the mRNA expression of FASN and DGAT2, the expression of ATGL and ACOX1 was also activated significantly and resulted in a decrease in cellular lipid deposition in goat intramuscular preadipocytes. Meanwhile, the silencing of MCD increased the cellular lipid deposition and was accompanied by the expression activation of DGAT2 and the expression suppression of ATGL and HSL, despite the expression suppression of genes related to fatty acid synthesis, including ACC and FASN. However, the expression of DGAT1 was not affected significantly (p > 0.05) by the expression alteration of MCD in this present study. Furthermore, 2025 bp of MCD promoter was obtained and predicted to be regulated by C/EBPα, SP1, SREBP1, and PPARG. In summary, although different pathways may respond to the expression alteration of MCD, the expression of MCD was negatively correlated with the cellular lipid deposition in goat intramuscular preadipocytes. These data may be beneficial for enhancing our understanding of the regulation of IMF deposition in goats. MDPI 2023-02-08 /pmc/articles/PMC9956415/ /pubmed/36833367 http://dx.doi.org/10.3390/genes14020440 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Changheng
Li, Qi
Lin, Yaqiu
Wang, Yong
Shi, Hengbo
Huang, Lian
Zhao, Wangsheng
Xiang, Hua
Zhu, Jiangjiang
MCD Inhibits Lipid Deposition in Goat Intramuscular Preadipocytes
title MCD Inhibits Lipid Deposition in Goat Intramuscular Preadipocytes
title_full MCD Inhibits Lipid Deposition in Goat Intramuscular Preadipocytes
title_fullStr MCD Inhibits Lipid Deposition in Goat Intramuscular Preadipocytes
title_full_unstemmed MCD Inhibits Lipid Deposition in Goat Intramuscular Preadipocytes
title_short MCD Inhibits Lipid Deposition in Goat Intramuscular Preadipocytes
title_sort mcd inhibits lipid deposition in goat intramuscular preadipocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9956415/
https://www.ncbi.nlm.nih.gov/pubmed/36833367
http://dx.doi.org/10.3390/genes14020440
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