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MCD Inhibits Lipid Deposition in Goat Intramuscular Preadipocytes
Malonyl-CoA decarboxylase (MCD) is a major regulator of fatty acid oxidation catalyzing the decarboxylation of malonyl coenzyme A (malonyl-CoA). Although its involvement in human diseases has been well studied, its role in intramuscular fat (IMF) deposition remains unknown. In this present study, 17...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9956415/ https://www.ncbi.nlm.nih.gov/pubmed/36833367 http://dx.doi.org/10.3390/genes14020440 |
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author | Yang, Changheng Li, Qi Lin, Yaqiu Wang, Yong Shi, Hengbo Huang, Lian Zhao, Wangsheng Xiang, Hua Zhu, Jiangjiang |
author_facet | Yang, Changheng Li, Qi Lin, Yaqiu Wang, Yong Shi, Hengbo Huang, Lian Zhao, Wangsheng Xiang, Hua Zhu, Jiangjiang |
author_sort | Yang, Changheng |
collection | PubMed |
description | Malonyl-CoA decarboxylase (MCD) is a major regulator of fatty acid oxidation catalyzing the decarboxylation of malonyl coenzyme A (malonyl-CoA). Although its involvement in human diseases has been well studied, its role in intramuscular fat (IMF) deposition remains unknown. In this present study, 1726 bp of MCD cDNA was cloned (OM937122) from goat liver, including 5′UTR of 27 bp, 3′UTR of 199 bp, and CDS of 1500 bp, encoding 499 amino acids. In this present study, although the overexpression of MCD increased the mRNA expression of FASN and DGAT2, the expression of ATGL and ACOX1 was also activated significantly and resulted in a decrease in cellular lipid deposition in goat intramuscular preadipocytes. Meanwhile, the silencing of MCD increased the cellular lipid deposition and was accompanied by the expression activation of DGAT2 and the expression suppression of ATGL and HSL, despite the expression suppression of genes related to fatty acid synthesis, including ACC and FASN. However, the expression of DGAT1 was not affected significantly (p > 0.05) by the expression alteration of MCD in this present study. Furthermore, 2025 bp of MCD promoter was obtained and predicted to be regulated by C/EBPα, SP1, SREBP1, and PPARG. In summary, although different pathways may respond to the expression alteration of MCD, the expression of MCD was negatively correlated with the cellular lipid deposition in goat intramuscular preadipocytes. These data may be beneficial for enhancing our understanding of the regulation of IMF deposition in goats. |
format | Online Article Text |
id | pubmed-9956415 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99564152023-02-25 MCD Inhibits Lipid Deposition in Goat Intramuscular Preadipocytes Yang, Changheng Li, Qi Lin, Yaqiu Wang, Yong Shi, Hengbo Huang, Lian Zhao, Wangsheng Xiang, Hua Zhu, Jiangjiang Genes (Basel) Article Malonyl-CoA decarboxylase (MCD) is a major regulator of fatty acid oxidation catalyzing the decarboxylation of malonyl coenzyme A (malonyl-CoA). Although its involvement in human diseases has been well studied, its role in intramuscular fat (IMF) deposition remains unknown. In this present study, 1726 bp of MCD cDNA was cloned (OM937122) from goat liver, including 5′UTR of 27 bp, 3′UTR of 199 bp, and CDS of 1500 bp, encoding 499 amino acids. In this present study, although the overexpression of MCD increased the mRNA expression of FASN and DGAT2, the expression of ATGL and ACOX1 was also activated significantly and resulted in a decrease in cellular lipid deposition in goat intramuscular preadipocytes. Meanwhile, the silencing of MCD increased the cellular lipid deposition and was accompanied by the expression activation of DGAT2 and the expression suppression of ATGL and HSL, despite the expression suppression of genes related to fatty acid synthesis, including ACC and FASN. However, the expression of DGAT1 was not affected significantly (p > 0.05) by the expression alteration of MCD in this present study. Furthermore, 2025 bp of MCD promoter was obtained and predicted to be regulated by C/EBPα, SP1, SREBP1, and PPARG. In summary, although different pathways may respond to the expression alteration of MCD, the expression of MCD was negatively correlated with the cellular lipid deposition in goat intramuscular preadipocytes. These data may be beneficial for enhancing our understanding of the regulation of IMF deposition in goats. MDPI 2023-02-08 /pmc/articles/PMC9956415/ /pubmed/36833367 http://dx.doi.org/10.3390/genes14020440 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Changheng Li, Qi Lin, Yaqiu Wang, Yong Shi, Hengbo Huang, Lian Zhao, Wangsheng Xiang, Hua Zhu, Jiangjiang MCD Inhibits Lipid Deposition in Goat Intramuscular Preadipocytes |
title | MCD Inhibits Lipid Deposition in Goat Intramuscular Preadipocytes |
title_full | MCD Inhibits Lipid Deposition in Goat Intramuscular Preadipocytes |
title_fullStr | MCD Inhibits Lipid Deposition in Goat Intramuscular Preadipocytes |
title_full_unstemmed | MCD Inhibits Lipid Deposition in Goat Intramuscular Preadipocytes |
title_short | MCD Inhibits Lipid Deposition in Goat Intramuscular Preadipocytes |
title_sort | mcd inhibits lipid deposition in goat intramuscular preadipocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9956415/ https://www.ncbi.nlm.nih.gov/pubmed/36833367 http://dx.doi.org/10.3390/genes14020440 |
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