Cargando…
Biosynthetic constraints on amino acid synthesis at the base of the food chain may determine their use in higher-order consumer genomes
Dietary nutrient composition is essential for shaping important fitness traits and behaviours. Many organisms are protein limited, and for Drosophila melanogaster this limitation manifests at the level of the single most limiting essential Amino Acid (AA) in the diet. The identity of this AA and its...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9956874/ https://www.ncbi.nlm.nih.gov/pubmed/36780875 http://dx.doi.org/10.1371/journal.pgen.1010635 |
_version_ | 1784894687571607552 |
---|---|
author | Gómez Ortega, Javier Raubenheimer, David Tyagi, Sonika Mirth, Christen K. Piper, Matthew D. W. |
author_facet | Gómez Ortega, Javier Raubenheimer, David Tyagi, Sonika Mirth, Christen K. Piper, Matthew D. W. |
author_sort | Gómez Ortega, Javier |
collection | PubMed |
description | Dietary nutrient composition is essential for shaping important fitness traits and behaviours. Many organisms are protein limited, and for Drosophila melanogaster this limitation manifests at the level of the single most limiting essential Amino Acid (AA) in the diet. The identity of this AA and its effects on female fecundity is readily predictable by a procedure called exome matching in which the sum of AAs encoded by a consumer’s exome is used to predict the relative proportion of AAs required in its diet. However, the exome matching calculation does not weight AA contributions to the overall profile by protein size or expression. Here, we update the exome matching calculation to include these weightings. Surprisingly, although nearly half of the transcriptome is differentially expressed when comparing male and female flies, we found that creating transcriptome-weighted exome matched diets for each sex did not enhance their fecundity over that supported by exome matching alone. These data indicate that while organisms may require different amounts of dietary protein across conditions, the relative proportion of the constituent AAs remains constant. Interestingly, we also found that exome matched AA profiles are generally conserved across taxa and that the composition of these profiles might be explained by energetic and elemental limitations on microbial AA synthesis. Thus, it appears that ecological constraints amongst autotrophs shape the relative proportion of AAs that are available across trophic levels and that this constrains biomass composition. |
format | Online Article Text |
id | pubmed-9956874 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-99568742023-02-25 Biosynthetic constraints on amino acid synthesis at the base of the food chain may determine their use in higher-order consumer genomes Gómez Ortega, Javier Raubenheimer, David Tyagi, Sonika Mirth, Christen K. Piper, Matthew D. W. PLoS Genet Research Article Dietary nutrient composition is essential for shaping important fitness traits and behaviours. Many organisms are protein limited, and for Drosophila melanogaster this limitation manifests at the level of the single most limiting essential Amino Acid (AA) in the diet. The identity of this AA and its effects on female fecundity is readily predictable by a procedure called exome matching in which the sum of AAs encoded by a consumer’s exome is used to predict the relative proportion of AAs required in its diet. However, the exome matching calculation does not weight AA contributions to the overall profile by protein size or expression. Here, we update the exome matching calculation to include these weightings. Surprisingly, although nearly half of the transcriptome is differentially expressed when comparing male and female flies, we found that creating transcriptome-weighted exome matched diets for each sex did not enhance their fecundity over that supported by exome matching alone. These data indicate that while organisms may require different amounts of dietary protein across conditions, the relative proportion of the constituent AAs remains constant. Interestingly, we also found that exome matched AA profiles are generally conserved across taxa and that the composition of these profiles might be explained by energetic and elemental limitations on microbial AA synthesis. Thus, it appears that ecological constraints amongst autotrophs shape the relative proportion of AAs that are available across trophic levels and that this constrains biomass composition. Public Library of Science 2023-02-13 /pmc/articles/PMC9956874/ /pubmed/36780875 http://dx.doi.org/10.1371/journal.pgen.1010635 Text en © 2023 Gómez Ortega et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Gómez Ortega, Javier Raubenheimer, David Tyagi, Sonika Mirth, Christen K. Piper, Matthew D. W. Biosynthetic constraints on amino acid synthesis at the base of the food chain may determine their use in higher-order consumer genomes |
title | Biosynthetic constraints on amino acid synthesis at the base of the food chain may determine their use in higher-order consumer genomes |
title_full | Biosynthetic constraints on amino acid synthesis at the base of the food chain may determine their use in higher-order consumer genomes |
title_fullStr | Biosynthetic constraints on amino acid synthesis at the base of the food chain may determine their use in higher-order consumer genomes |
title_full_unstemmed | Biosynthetic constraints on amino acid synthesis at the base of the food chain may determine their use in higher-order consumer genomes |
title_short | Biosynthetic constraints on amino acid synthesis at the base of the food chain may determine their use in higher-order consumer genomes |
title_sort | biosynthetic constraints on amino acid synthesis at the base of the food chain may determine their use in higher-order consumer genomes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9956874/ https://www.ncbi.nlm.nih.gov/pubmed/36780875 http://dx.doi.org/10.1371/journal.pgen.1010635 |
work_keys_str_mv | AT gomezortegajavier biosyntheticconstraintsonaminoacidsynthesisatthebaseofthefoodchainmaydeterminetheiruseinhigherorderconsumergenomes AT raubenheimerdavid biosyntheticconstraintsonaminoacidsynthesisatthebaseofthefoodchainmaydeterminetheiruseinhigherorderconsumergenomes AT tyagisonika biosyntheticconstraintsonaminoacidsynthesisatthebaseofthefoodchainmaydeterminetheiruseinhigherorderconsumergenomes AT mirthchristenk biosyntheticconstraintsonaminoacidsynthesisatthebaseofthefoodchainmaydeterminetheiruseinhigherorderconsumergenomes AT pipermatthewdw biosyntheticconstraintsonaminoacidsynthesisatthebaseofthefoodchainmaydeterminetheiruseinhigherorderconsumergenomes |