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OCT and ERG Techniques in High-Throughput Phenotyping of Mouse Vision
The purpose of the study is to demonstrate coherent optical tomography and electroretinography techniques adopted from the human clinical practice to assess the morphology and function of the mouse retina in a high-throughput phenotyping environment. We present the normal range of wild-type C57Bl/6N...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9956909/ https://www.ncbi.nlm.nih.gov/pubmed/36833221 http://dx.doi.org/10.3390/genes14020294 |
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author | Lindovsky, Jiri Palkova, Marcela Symkina, Viktoriia Raishbrook, Miles Joseph Prochazka, Jan Sedlacek, Radislav |
author_facet | Lindovsky, Jiri Palkova, Marcela Symkina, Viktoriia Raishbrook, Miles Joseph Prochazka, Jan Sedlacek, Radislav |
author_sort | Lindovsky, Jiri |
collection | PubMed |
description | The purpose of the study is to demonstrate coherent optical tomography and electroretinography techniques adopted from the human clinical practice to assess the morphology and function of the mouse retina in a high-throughput phenotyping environment. We present the normal range of wild-type C57Bl/6NCrl retinal parameters in six age groups between 10 and 100 weeks as well as examples of mild and severe pathologies resulting from knocking out a single protein-coding gene. We also show example data obtained by more detailed analysis or additional methods useful in eye research, for example, the angiography of a superficial and deep vascular complex. We discuss the feasibility of these techniques in conditions demanding a high-throughput approach such as the systemic phenotyping carried out by the International Mouse Phenotyping Consortium. |
format | Online Article Text |
id | pubmed-9956909 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99569092023-02-25 OCT and ERG Techniques in High-Throughput Phenotyping of Mouse Vision Lindovsky, Jiri Palkova, Marcela Symkina, Viktoriia Raishbrook, Miles Joseph Prochazka, Jan Sedlacek, Radislav Genes (Basel) Article The purpose of the study is to demonstrate coherent optical tomography and electroretinography techniques adopted from the human clinical practice to assess the morphology and function of the mouse retina in a high-throughput phenotyping environment. We present the normal range of wild-type C57Bl/6NCrl retinal parameters in six age groups between 10 and 100 weeks as well as examples of mild and severe pathologies resulting from knocking out a single protein-coding gene. We also show example data obtained by more detailed analysis or additional methods useful in eye research, for example, the angiography of a superficial and deep vascular complex. We discuss the feasibility of these techniques in conditions demanding a high-throughput approach such as the systemic phenotyping carried out by the International Mouse Phenotyping Consortium. MDPI 2023-01-22 /pmc/articles/PMC9956909/ /pubmed/36833221 http://dx.doi.org/10.3390/genes14020294 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lindovsky, Jiri Palkova, Marcela Symkina, Viktoriia Raishbrook, Miles Joseph Prochazka, Jan Sedlacek, Radislav OCT and ERG Techniques in High-Throughput Phenotyping of Mouse Vision |
title | OCT and ERG Techniques in High-Throughput Phenotyping of Mouse Vision |
title_full | OCT and ERG Techniques in High-Throughput Phenotyping of Mouse Vision |
title_fullStr | OCT and ERG Techniques in High-Throughput Phenotyping of Mouse Vision |
title_full_unstemmed | OCT and ERG Techniques in High-Throughput Phenotyping of Mouse Vision |
title_short | OCT and ERG Techniques in High-Throughput Phenotyping of Mouse Vision |
title_sort | oct and erg techniques in high-throughput phenotyping of mouse vision |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9956909/ https://www.ncbi.nlm.nih.gov/pubmed/36833221 http://dx.doi.org/10.3390/genes14020294 |
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