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Genome-Wide Identification, Evolutionary and Functional Analyses of WRKY Family Members in Ginkgo biloba
WRKY transcription factors (TFs) are one of the largest families in plants which play essential roles in plant growth and stress response. Ginkgo biloba is a living fossil that has remained essentially unchanged for more than 200 million years, and now has become widespread worldwide due to the medi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9956969/ https://www.ncbi.nlm.nih.gov/pubmed/36833270 http://dx.doi.org/10.3390/genes14020343 |
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author | Li, Weixing Xiao, Nan Wang, Yawen Liu, Ximeng Chen, Zhaoyu Gu, Xiaoyin Chen, Yadi |
author_facet | Li, Weixing Xiao, Nan Wang, Yawen Liu, Ximeng Chen, Zhaoyu Gu, Xiaoyin Chen, Yadi |
author_sort | Li, Weixing |
collection | PubMed |
description | WRKY transcription factors (TFs) are one of the largest families in plants which play essential roles in plant growth and stress response. Ginkgo biloba is a living fossil that has remained essentially unchanged for more than 200 million years, and now has become widespread worldwide due to the medicinal active ingredients in its leaves. Here, 37 WRKY genes were identified, which were distributed randomly in nine chromosomes of G. biloba. Results of the phylogenetic analysis indicated that the GbWRKY could be divided into three groups. Furthermore, the expression patterns of GbWRKY genes were analyzed. Gene expression profiling and qRT−PCR revealed that different members of GbWRKY have different spatiotemporal expression patterns in different abiotic stresses. Most of the GbWRKY genes can respond to UV-B radiation, drought, high temperature and salt treatment. Meanwhile, all GbWRKY members performed phylogenetic tree analyses with the WRKY proteins of other species which were known to be associated with abiotic stress. The result suggested that GbWRKY may play a crucial role in regulating multiple stress tolerances. Additionally, GbWRKY13 and GbWRKY37 were all located in the nucleus, while GbWRKY15 was located in the nucleus and cytomembrane. |
format | Online Article Text |
id | pubmed-9956969 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99569692023-02-25 Genome-Wide Identification, Evolutionary and Functional Analyses of WRKY Family Members in Ginkgo biloba Li, Weixing Xiao, Nan Wang, Yawen Liu, Ximeng Chen, Zhaoyu Gu, Xiaoyin Chen, Yadi Genes (Basel) Article WRKY transcription factors (TFs) are one of the largest families in plants which play essential roles in plant growth and stress response. Ginkgo biloba is a living fossil that has remained essentially unchanged for more than 200 million years, and now has become widespread worldwide due to the medicinal active ingredients in its leaves. Here, 37 WRKY genes were identified, which were distributed randomly in nine chromosomes of G. biloba. Results of the phylogenetic analysis indicated that the GbWRKY could be divided into three groups. Furthermore, the expression patterns of GbWRKY genes were analyzed. Gene expression profiling and qRT−PCR revealed that different members of GbWRKY have different spatiotemporal expression patterns in different abiotic stresses. Most of the GbWRKY genes can respond to UV-B radiation, drought, high temperature and salt treatment. Meanwhile, all GbWRKY members performed phylogenetic tree analyses with the WRKY proteins of other species which were known to be associated with abiotic stress. The result suggested that GbWRKY may play a crucial role in regulating multiple stress tolerances. Additionally, GbWRKY13 and GbWRKY37 were all located in the nucleus, while GbWRKY15 was located in the nucleus and cytomembrane. MDPI 2023-01-28 /pmc/articles/PMC9956969/ /pubmed/36833270 http://dx.doi.org/10.3390/genes14020343 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Weixing Xiao, Nan Wang, Yawen Liu, Ximeng Chen, Zhaoyu Gu, Xiaoyin Chen, Yadi Genome-Wide Identification, Evolutionary and Functional Analyses of WRKY Family Members in Ginkgo biloba |
title | Genome-Wide Identification, Evolutionary and Functional Analyses of WRKY Family Members in Ginkgo biloba |
title_full | Genome-Wide Identification, Evolutionary and Functional Analyses of WRKY Family Members in Ginkgo biloba |
title_fullStr | Genome-Wide Identification, Evolutionary and Functional Analyses of WRKY Family Members in Ginkgo biloba |
title_full_unstemmed | Genome-Wide Identification, Evolutionary and Functional Analyses of WRKY Family Members in Ginkgo biloba |
title_short | Genome-Wide Identification, Evolutionary and Functional Analyses of WRKY Family Members in Ginkgo biloba |
title_sort | genome-wide identification, evolutionary and functional analyses of wrky family members in ginkgo biloba |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9956969/ https://www.ncbi.nlm.nih.gov/pubmed/36833270 http://dx.doi.org/10.3390/genes14020343 |
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