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miR-33a Inhibits the Differentiation of Bovine Preadipocytes through the IRS2–Akt Pathway
Several microRNAs (miRNAs) are known to participate in adipogenesis. However, their role in this process, especially in the differentiation of bovine preadipocytes, remains to be elucidated. This study was intended to clarify the effect of microRNA-33a (miR-33a) on the differentiation of bovine prea...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9957011/ https://www.ncbi.nlm.nih.gov/pubmed/36833456 http://dx.doi.org/10.3390/genes14020529 |
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author | Zhang, Wenzhen Raza, Sayed Haidar Abbas Li, Bingzhi Sun, Bing Wang, Sihu Pant, Sameer D. Al-Abbas, Nouf S. Shaer, Nehad A. Zan, Linsen |
author_facet | Zhang, Wenzhen Raza, Sayed Haidar Abbas Li, Bingzhi Sun, Bing Wang, Sihu Pant, Sameer D. Al-Abbas, Nouf S. Shaer, Nehad A. Zan, Linsen |
author_sort | Zhang, Wenzhen |
collection | PubMed |
description | Several microRNAs (miRNAs) are known to participate in adipogenesis. However, their role in this process, especially in the differentiation of bovine preadipocytes, remains to be elucidated. This study was intended to clarify the effect of microRNA-33a (miR-33a) on the differentiation of bovine preadipocytes by cell culture, real-time fluorescent quantitative PCR (qPCR), Oil Red staining, BODIPY staining, and Western blotting. The results indicate that overexpression of miR-33a significantly inhibited lipid droplet accumulation and decreased the mRNA and protein expression of adipocyte differentiation marker genes such as peroxisome proliferator-activated receptor gamma (PPARγ), sterol regulatory element-binding protein 1 (SREBP1), and fatty acid-binding protein 4 (FABP4). In contrast, the interference expression of miR-33a promoted lipid droplet accumulation and increased the expression of marker genes. Additionally, miR-33a directly targeted insulin receptor substrate 2 (IRS2) and regulated the phosphorylation level of serine/threonine kinase (Akt). Furthermore, miR-33a inhibition could rescue defects in the differentiation of bovine preadipocytes and the Akt phosphorylation level caused by small interfering IRS2 (si-IRS2). Collectively, these results indicate that miR-33a could inhibit the differentiation of bovine preadipocytes, possibly through the IRS2–Akt pathway. These findings might help develop practical means to improve the quality of beef. |
format | Online Article Text |
id | pubmed-9957011 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99570112023-02-25 miR-33a Inhibits the Differentiation of Bovine Preadipocytes through the IRS2–Akt Pathway Zhang, Wenzhen Raza, Sayed Haidar Abbas Li, Bingzhi Sun, Bing Wang, Sihu Pant, Sameer D. Al-Abbas, Nouf S. Shaer, Nehad A. Zan, Linsen Genes (Basel) Article Several microRNAs (miRNAs) are known to participate in adipogenesis. However, their role in this process, especially in the differentiation of bovine preadipocytes, remains to be elucidated. This study was intended to clarify the effect of microRNA-33a (miR-33a) on the differentiation of bovine preadipocytes by cell culture, real-time fluorescent quantitative PCR (qPCR), Oil Red staining, BODIPY staining, and Western blotting. The results indicate that overexpression of miR-33a significantly inhibited lipid droplet accumulation and decreased the mRNA and protein expression of adipocyte differentiation marker genes such as peroxisome proliferator-activated receptor gamma (PPARγ), sterol regulatory element-binding protein 1 (SREBP1), and fatty acid-binding protein 4 (FABP4). In contrast, the interference expression of miR-33a promoted lipid droplet accumulation and increased the expression of marker genes. Additionally, miR-33a directly targeted insulin receptor substrate 2 (IRS2) and regulated the phosphorylation level of serine/threonine kinase (Akt). Furthermore, miR-33a inhibition could rescue defects in the differentiation of bovine preadipocytes and the Akt phosphorylation level caused by small interfering IRS2 (si-IRS2). Collectively, these results indicate that miR-33a could inhibit the differentiation of bovine preadipocytes, possibly through the IRS2–Akt pathway. These findings might help develop practical means to improve the quality of beef. MDPI 2023-02-20 /pmc/articles/PMC9957011/ /pubmed/36833456 http://dx.doi.org/10.3390/genes14020529 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Wenzhen Raza, Sayed Haidar Abbas Li, Bingzhi Sun, Bing Wang, Sihu Pant, Sameer D. Al-Abbas, Nouf S. Shaer, Nehad A. Zan, Linsen miR-33a Inhibits the Differentiation of Bovine Preadipocytes through the IRS2–Akt Pathway |
title | miR-33a Inhibits the Differentiation of Bovine Preadipocytes through the IRS2–Akt Pathway |
title_full | miR-33a Inhibits the Differentiation of Bovine Preadipocytes through the IRS2–Akt Pathway |
title_fullStr | miR-33a Inhibits the Differentiation of Bovine Preadipocytes through the IRS2–Akt Pathway |
title_full_unstemmed | miR-33a Inhibits the Differentiation of Bovine Preadipocytes through the IRS2–Akt Pathway |
title_short | miR-33a Inhibits the Differentiation of Bovine Preadipocytes through the IRS2–Akt Pathway |
title_sort | mir-33a inhibits the differentiation of bovine preadipocytes through the irs2–akt pathway |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9957011/ https://www.ncbi.nlm.nih.gov/pubmed/36833456 http://dx.doi.org/10.3390/genes14020529 |
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