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Efficacy of 5% Aminolaevulinic Acid and Red Light on Enterococcus faecalis in Infected Root Canals

Background: In this ex vivo study, the aim was to evaluate the effects of ALAD and red light on Enterococcus faecalis in infected root canals using a special intracanal fiber. Methods: A total of 70 extracted, single-rooted teeth were used. The teeth were decoronated at the length of the roots to ap...

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Autores principales: Carlesi, Teocrito, Dotta, Tatiane Cristina, Pierfelice, Tania Vanessa, D’Amico, Emira, Lepore, Stefania, Tripodi, Domenico, Piattelli, Adriano, D’Ercole, Simonetta, Petrini, Morena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9957220/
https://www.ncbi.nlm.nih.gov/pubmed/36826295
http://dx.doi.org/10.3390/gels9020125
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author Carlesi, Teocrito
Dotta, Tatiane Cristina
Pierfelice, Tania Vanessa
D’Amico, Emira
Lepore, Stefania
Tripodi, Domenico
Piattelli, Adriano
D’Ercole, Simonetta
Petrini, Morena
author_facet Carlesi, Teocrito
Dotta, Tatiane Cristina
Pierfelice, Tania Vanessa
D’Amico, Emira
Lepore, Stefania
Tripodi, Domenico
Piattelli, Adriano
D’Ercole, Simonetta
Petrini, Morena
author_sort Carlesi, Teocrito
collection PubMed
description Background: In this ex vivo study, the aim was to evaluate the effects of ALAD and red light on Enterococcus faecalis in infected root canals using a special intracanal fiber. Methods: A total of 70 extracted, single-rooted teeth were used. The teeth were decoronated at the length of the roots to approximately 15 mm and then instrumented. The apical foramen was sealed by composite resin, and the root canals were infected with a pure culture of E. faecalis ATCC 29212 for eight days at 37 °C. Following the contamination period, the roots were divided into seven groups, including the positive and negative control groups, and treated as follows: ALAD 45 min; red light activation 7 min; ALAD 45 min and red-light activation 7 min; sodium hypochlorite 2.5% 15 min; sodium hypochlorite 1% 15 min. The samples were taken by three sterile paper points, transferred to tubes containing 1 mL of PBS, and immediately processed for the number of colony-forming units and the cell viability by using live/dead. Results: The best treatment is obtained with 2.5% NaOCl. Except for ALAD + red light vs. 1% NaOCl, a statistically significant difference is recorded for all treatments. The combination of 2.5% NaOCl and ALAD + 7 min irradiation produces an evident killing effect on the E. faecalis cells. On the other hand, 1% NaOCl is ineffective for the viability action, with 25% of dead cells stained in red. Conclusions: This ex vivo study shows that ALAD gel with light irradiation is an efficacious protocol that exerts a potent antibacterial activity against E. faecalis in infected root canals.
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spelling pubmed-99572202023-02-25 Efficacy of 5% Aminolaevulinic Acid and Red Light on Enterococcus faecalis in Infected Root Canals Carlesi, Teocrito Dotta, Tatiane Cristina Pierfelice, Tania Vanessa D’Amico, Emira Lepore, Stefania Tripodi, Domenico Piattelli, Adriano D’Ercole, Simonetta Petrini, Morena Gels Article Background: In this ex vivo study, the aim was to evaluate the effects of ALAD and red light on Enterococcus faecalis in infected root canals using a special intracanal fiber. Methods: A total of 70 extracted, single-rooted teeth were used. The teeth were decoronated at the length of the roots to approximately 15 mm and then instrumented. The apical foramen was sealed by composite resin, and the root canals were infected with a pure culture of E. faecalis ATCC 29212 for eight days at 37 °C. Following the contamination period, the roots were divided into seven groups, including the positive and negative control groups, and treated as follows: ALAD 45 min; red light activation 7 min; ALAD 45 min and red-light activation 7 min; sodium hypochlorite 2.5% 15 min; sodium hypochlorite 1% 15 min. The samples were taken by three sterile paper points, transferred to tubes containing 1 mL of PBS, and immediately processed for the number of colony-forming units and the cell viability by using live/dead. Results: The best treatment is obtained with 2.5% NaOCl. Except for ALAD + red light vs. 1% NaOCl, a statistically significant difference is recorded for all treatments. The combination of 2.5% NaOCl and ALAD + 7 min irradiation produces an evident killing effect on the E. faecalis cells. On the other hand, 1% NaOCl is ineffective for the viability action, with 25% of dead cells stained in red. Conclusions: This ex vivo study shows that ALAD gel with light irradiation is an efficacious protocol that exerts a potent antibacterial activity against E. faecalis in infected root canals. MDPI 2023-02-02 /pmc/articles/PMC9957220/ /pubmed/36826295 http://dx.doi.org/10.3390/gels9020125 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Carlesi, Teocrito
Dotta, Tatiane Cristina
Pierfelice, Tania Vanessa
D’Amico, Emira
Lepore, Stefania
Tripodi, Domenico
Piattelli, Adriano
D’Ercole, Simonetta
Petrini, Morena
Efficacy of 5% Aminolaevulinic Acid and Red Light on Enterococcus faecalis in Infected Root Canals
title Efficacy of 5% Aminolaevulinic Acid and Red Light on Enterococcus faecalis in Infected Root Canals
title_full Efficacy of 5% Aminolaevulinic Acid and Red Light on Enterococcus faecalis in Infected Root Canals
title_fullStr Efficacy of 5% Aminolaevulinic Acid and Red Light on Enterococcus faecalis in Infected Root Canals
title_full_unstemmed Efficacy of 5% Aminolaevulinic Acid and Red Light on Enterococcus faecalis in Infected Root Canals
title_short Efficacy of 5% Aminolaevulinic Acid and Red Light on Enterococcus faecalis in Infected Root Canals
title_sort efficacy of 5% aminolaevulinic acid and red light on enterococcus faecalis in infected root canals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9957220/
https://www.ncbi.nlm.nih.gov/pubmed/36826295
http://dx.doi.org/10.3390/gels9020125
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