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Genome-Wide Identification, Characterization and Expression Profiling of Potato (Solanum tuberosum) Frataxin (FH) Gene

Frataxin (FH) plays a crucial role in the biogenesis of mitochondria and the regulation of iron in the cells of various organisms. However, there has been very little research on FH in plants. In this study, the potato FH gene (StFH) was identified and characterized using a genome-wide approach, and...

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Autores principales: Kurt, Firat, Filiz, Ertugrul, Yildiz, Kubra, Akbudak, M. Aydın
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9957314/
https://www.ncbi.nlm.nih.gov/pubmed/36833395
http://dx.doi.org/10.3390/genes14020468
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author Kurt, Firat
Filiz, Ertugrul
Yildiz, Kubra
Akbudak, M. Aydın
author_facet Kurt, Firat
Filiz, Ertugrul
Yildiz, Kubra
Akbudak, M. Aydın
author_sort Kurt, Firat
collection PubMed
description Frataxin (FH) plays a crucial role in the biogenesis of mitochondria and the regulation of iron in the cells of various organisms. However, there has been very little research on FH in plants. In this study, the potato FH gene (StFH) was identified and characterized using a genome-wide approach, and its sequence was compared to those of FH genes from Arabidopsis, rice, and maize. The FH genes were found to have a lineage-specific distribution and were more conserved in monocots than in dicots. While multiple copies of FH genes have been reported in some species, including plants, only one isoform of FH was found in potato. The expression of StFH in leaves and roots was analyzed under two different abiotic stress conditions, and the results showed that StFH was upregulated more in leaves and that its expression levels increased with the severity of the stress. This is the first study to examine the expression of an FH gene under abiotic stress conditions.
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spelling pubmed-99573142023-02-25 Genome-Wide Identification, Characterization and Expression Profiling of Potato (Solanum tuberosum) Frataxin (FH) Gene Kurt, Firat Filiz, Ertugrul Yildiz, Kubra Akbudak, M. Aydın Genes (Basel) Article Frataxin (FH) plays a crucial role in the biogenesis of mitochondria and the regulation of iron in the cells of various organisms. However, there has been very little research on FH in plants. In this study, the potato FH gene (StFH) was identified and characterized using a genome-wide approach, and its sequence was compared to those of FH genes from Arabidopsis, rice, and maize. The FH genes were found to have a lineage-specific distribution and were more conserved in monocots than in dicots. While multiple copies of FH genes have been reported in some species, including plants, only one isoform of FH was found in potato. The expression of StFH in leaves and roots was analyzed under two different abiotic stress conditions, and the results showed that StFH was upregulated more in leaves and that its expression levels increased with the severity of the stress. This is the first study to examine the expression of an FH gene under abiotic stress conditions. MDPI 2023-02-11 /pmc/articles/PMC9957314/ /pubmed/36833395 http://dx.doi.org/10.3390/genes14020468 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kurt, Firat
Filiz, Ertugrul
Yildiz, Kubra
Akbudak, M. Aydın
Genome-Wide Identification, Characterization and Expression Profiling of Potato (Solanum tuberosum) Frataxin (FH) Gene
title Genome-Wide Identification, Characterization and Expression Profiling of Potato (Solanum tuberosum) Frataxin (FH) Gene
title_full Genome-Wide Identification, Characterization and Expression Profiling of Potato (Solanum tuberosum) Frataxin (FH) Gene
title_fullStr Genome-Wide Identification, Characterization and Expression Profiling of Potato (Solanum tuberosum) Frataxin (FH) Gene
title_full_unstemmed Genome-Wide Identification, Characterization and Expression Profiling of Potato (Solanum tuberosum) Frataxin (FH) Gene
title_short Genome-Wide Identification, Characterization and Expression Profiling of Potato (Solanum tuberosum) Frataxin (FH) Gene
title_sort genome-wide identification, characterization and expression profiling of potato (solanum tuberosum) frataxin (fh) gene
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9957314/
https://www.ncbi.nlm.nih.gov/pubmed/36833395
http://dx.doi.org/10.3390/genes14020468
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