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Characterization and Expression of Holothurian Wnt Signaling Genes during Adult Intestinal Organogenesis
Wnt signaling has been shown to play multiple roles in regenerative processes, one of the most widely studied of which is the regeneration of the intestinal luminal epithelia. Most studies in this area have focused on self-renewal of the luminal stem cells; however, Wnt signaling may also have more...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9957329/ https://www.ncbi.nlm.nih.gov/pubmed/36833237 http://dx.doi.org/10.3390/genes14020309 |
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author | Auger, Noah A. Medina-Feliciano, Joshua G. Quispe-Parra, David J. Colón-Marrero, Stephanie Ortiz-Zuazaga, Humberto García-Arrarás, José E. |
author_facet | Auger, Noah A. Medina-Feliciano, Joshua G. Quispe-Parra, David J. Colón-Marrero, Stephanie Ortiz-Zuazaga, Humberto García-Arrarás, José E. |
author_sort | Auger, Noah A. |
collection | PubMed |
description | Wnt signaling has been shown to play multiple roles in regenerative processes, one of the most widely studied of which is the regeneration of the intestinal luminal epithelia. Most studies in this area have focused on self-renewal of the luminal stem cells; however, Wnt signaling may also have more dynamic functions, such as facilitating intestinal organogenesis. To explore this possibility, we employed the sea cucumber Holothuria glaberrima that can regenerate a full intestine over the course of 21 days after evisceration. We collected RNA-seq data from various intestinal tissues and regeneration stages and used these data to define the Wnt genes present in H. glaberrima and the differential gene expression (DGE) patterns during the regenerative process. Twelve Wnt genes were found, and their presence was confirmed in the draft genome of H. glaberrima. The expressions of additional Wnt-associated genes, such as Frizzled and Disheveled, as well as genes from the Wnt/β-catenin and Wnt/Planar Cell Polarity (PCP) pathways, were also analyzed. DGE showed unique distributions of Wnt in early- and late-stage intestinal regenerates, consistent with the Wnt/β-catenin pathway being upregulated during early-stages and the Wnt/PCP pathway being upregulated during late-stages. Our results demonstrate the diversity of Wnt signaling during intestinal regeneration, highlighting possible roles in adult organogenesis. |
format | Online Article Text |
id | pubmed-9957329 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99573292023-02-25 Characterization and Expression of Holothurian Wnt Signaling Genes during Adult Intestinal Organogenesis Auger, Noah A. Medina-Feliciano, Joshua G. Quispe-Parra, David J. Colón-Marrero, Stephanie Ortiz-Zuazaga, Humberto García-Arrarás, José E. Genes (Basel) Article Wnt signaling has been shown to play multiple roles in regenerative processes, one of the most widely studied of which is the regeneration of the intestinal luminal epithelia. Most studies in this area have focused on self-renewal of the luminal stem cells; however, Wnt signaling may also have more dynamic functions, such as facilitating intestinal organogenesis. To explore this possibility, we employed the sea cucumber Holothuria glaberrima that can regenerate a full intestine over the course of 21 days after evisceration. We collected RNA-seq data from various intestinal tissues and regeneration stages and used these data to define the Wnt genes present in H. glaberrima and the differential gene expression (DGE) patterns during the regenerative process. Twelve Wnt genes were found, and their presence was confirmed in the draft genome of H. glaberrima. The expressions of additional Wnt-associated genes, such as Frizzled and Disheveled, as well as genes from the Wnt/β-catenin and Wnt/Planar Cell Polarity (PCP) pathways, were also analyzed. DGE showed unique distributions of Wnt in early- and late-stage intestinal regenerates, consistent with the Wnt/β-catenin pathway being upregulated during early-stages and the Wnt/PCP pathway being upregulated during late-stages. Our results demonstrate the diversity of Wnt signaling during intestinal regeneration, highlighting possible roles in adult organogenesis. MDPI 2023-01-25 /pmc/articles/PMC9957329/ /pubmed/36833237 http://dx.doi.org/10.3390/genes14020309 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Auger, Noah A. Medina-Feliciano, Joshua G. Quispe-Parra, David J. Colón-Marrero, Stephanie Ortiz-Zuazaga, Humberto García-Arrarás, José E. Characterization and Expression of Holothurian Wnt Signaling Genes during Adult Intestinal Organogenesis |
title | Characterization and Expression of Holothurian Wnt Signaling Genes during Adult Intestinal Organogenesis |
title_full | Characterization and Expression of Holothurian Wnt Signaling Genes during Adult Intestinal Organogenesis |
title_fullStr | Characterization and Expression of Holothurian Wnt Signaling Genes during Adult Intestinal Organogenesis |
title_full_unstemmed | Characterization and Expression of Holothurian Wnt Signaling Genes during Adult Intestinal Organogenesis |
title_short | Characterization and Expression of Holothurian Wnt Signaling Genes during Adult Intestinal Organogenesis |
title_sort | characterization and expression of holothurian wnt signaling genes during adult intestinal organogenesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9957329/ https://www.ncbi.nlm.nih.gov/pubmed/36833237 http://dx.doi.org/10.3390/genes14020309 |
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