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Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial

Diabetic patients frequently develop wounds, which can be colonized by bacteria, mainly Staphylococcus aureus and Pseudomonas aeruginosa, with the ability to form biofilms. This study aimed to evaluate the colonization and biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa in chro...

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Autores principales: Pessanha, Fernanda Soares, de Oliveira, Beatriz Guitton Renaud Baptista, Oliveira, Bianca Campos, Deutsch, Gabriela, Teixeira, Felipe Lopes, Bokehi, Luciana Castilho, Calomino, Mariana Alcântara, Rodrigues de Castilho, Selma, Thiré, Rossana Mara da Silva Moreira, Teixeira, Lenise Arneiro, de Paula, Geraldo Renato
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9957415/
https://www.ncbi.nlm.nih.gov/pubmed/36826286
http://dx.doi.org/10.3390/gels9020117
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author Pessanha, Fernanda Soares
de Oliveira, Beatriz Guitton Renaud Baptista
Oliveira, Bianca Campos
Deutsch, Gabriela
Teixeira, Felipe Lopes
Bokehi, Luciana Castilho
Calomino, Mariana Alcântara
Rodrigues de Castilho, Selma
Thiré, Rossana Mara da Silva Moreira
Teixeira, Lenise Arneiro
de Paula, Geraldo Renato
author_facet Pessanha, Fernanda Soares
de Oliveira, Beatriz Guitton Renaud Baptista
Oliveira, Bianca Campos
Deutsch, Gabriela
Teixeira, Felipe Lopes
Bokehi, Luciana Castilho
Calomino, Mariana Alcântara
Rodrigues de Castilho, Selma
Thiré, Rossana Mara da Silva Moreira
Teixeira, Lenise Arneiro
de Paula, Geraldo Renato
author_sort Pessanha, Fernanda Soares
collection PubMed
description Diabetic patients frequently develop wounds, which can be colonized by bacteria, mainly Staphylococcus aureus and Pseudomonas aeruginosa, with the ability to form biofilms. This study aimed to evaluate the colonization and biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa in chronic wounds of diabetic patients treated with a bioactive dressing (EGF-CMC), which consisted of a 2% carboxymethylcellulose (CMC) hydrogel loaded with epidermal growth factor (EGF). This randomized clinical trial was conducted with 25 participants: 14 treated with EGF-CMC hydrogel and 11 treated with CMC hydrogel for 12 weeks. Participants with type 2 diabetes mellitus were selected. All had diabetic foot ulcers or chronic venous ulcers. Swab collections were performed on weeks 1, 6, and 12. The laboratory analyses included the identification of strains, microbial quantification, virulence gene investigation, and the evaluation of biofilm formation. In total, 13 S. aureus strains and 15 P. aeruginosa strains were isolated. There were no statistically significant differences regarding bacterial loads and virulence genes. However, EGF-CMC-hydrogel-treated wounds were colonized by strains with lower biofilm formation abilities. The probability of isolating biofilm-producing strains from CMC-hydrogel-treated wounds was 83% greater than the probability of isolating biofilm-producing strains from EGF-CMC-treated wounds.
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spelling pubmed-99574152023-02-25 Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial Pessanha, Fernanda Soares de Oliveira, Beatriz Guitton Renaud Baptista Oliveira, Bianca Campos Deutsch, Gabriela Teixeira, Felipe Lopes Bokehi, Luciana Castilho Calomino, Mariana Alcântara Rodrigues de Castilho, Selma Thiré, Rossana Mara da Silva Moreira Teixeira, Lenise Arneiro de Paula, Geraldo Renato Gels Article Diabetic patients frequently develop wounds, which can be colonized by bacteria, mainly Staphylococcus aureus and Pseudomonas aeruginosa, with the ability to form biofilms. This study aimed to evaluate the colonization and biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa in chronic wounds of diabetic patients treated with a bioactive dressing (EGF-CMC), which consisted of a 2% carboxymethylcellulose (CMC) hydrogel loaded with epidermal growth factor (EGF). This randomized clinical trial was conducted with 25 participants: 14 treated with EGF-CMC hydrogel and 11 treated with CMC hydrogel for 12 weeks. Participants with type 2 diabetes mellitus were selected. All had diabetic foot ulcers or chronic venous ulcers. Swab collections were performed on weeks 1, 6, and 12. The laboratory analyses included the identification of strains, microbial quantification, virulence gene investigation, and the evaluation of biofilm formation. In total, 13 S. aureus strains and 15 P. aeruginosa strains were isolated. There were no statistically significant differences regarding bacterial loads and virulence genes. However, EGF-CMC-hydrogel-treated wounds were colonized by strains with lower biofilm formation abilities. The probability of isolating biofilm-producing strains from CMC-hydrogel-treated wounds was 83% greater than the probability of isolating biofilm-producing strains from EGF-CMC-treated wounds. MDPI 2023-02-01 /pmc/articles/PMC9957415/ /pubmed/36826286 http://dx.doi.org/10.3390/gels9020117 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pessanha, Fernanda Soares
de Oliveira, Beatriz Guitton Renaud Baptista
Oliveira, Bianca Campos
Deutsch, Gabriela
Teixeira, Felipe Lopes
Bokehi, Luciana Castilho
Calomino, Mariana Alcântara
Rodrigues de Castilho, Selma
Thiré, Rossana Mara da Silva Moreira
Teixeira, Lenise Arneiro
de Paula, Geraldo Renato
Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial
title Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial
title_full Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial
title_fullStr Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial
title_full_unstemmed Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial
title_short Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial
title_sort effectiveness of epidermal growth factor loaded carboxymethylcellulose (egf-cmc) hydrogel in biofilm formation in wounds of diabetic patients: a randomized clinical trial
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9957415/
https://www.ncbi.nlm.nih.gov/pubmed/36826286
http://dx.doi.org/10.3390/gels9020117
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