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Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial
Diabetic patients frequently develop wounds, which can be colonized by bacteria, mainly Staphylococcus aureus and Pseudomonas aeruginosa, with the ability to form biofilms. This study aimed to evaluate the colonization and biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa in chro...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9957415/ https://www.ncbi.nlm.nih.gov/pubmed/36826286 http://dx.doi.org/10.3390/gels9020117 |
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author | Pessanha, Fernanda Soares de Oliveira, Beatriz Guitton Renaud Baptista Oliveira, Bianca Campos Deutsch, Gabriela Teixeira, Felipe Lopes Bokehi, Luciana Castilho Calomino, Mariana Alcântara Rodrigues de Castilho, Selma Thiré, Rossana Mara da Silva Moreira Teixeira, Lenise Arneiro de Paula, Geraldo Renato |
author_facet | Pessanha, Fernanda Soares de Oliveira, Beatriz Guitton Renaud Baptista Oliveira, Bianca Campos Deutsch, Gabriela Teixeira, Felipe Lopes Bokehi, Luciana Castilho Calomino, Mariana Alcântara Rodrigues de Castilho, Selma Thiré, Rossana Mara da Silva Moreira Teixeira, Lenise Arneiro de Paula, Geraldo Renato |
author_sort | Pessanha, Fernanda Soares |
collection | PubMed |
description | Diabetic patients frequently develop wounds, which can be colonized by bacteria, mainly Staphylococcus aureus and Pseudomonas aeruginosa, with the ability to form biofilms. This study aimed to evaluate the colonization and biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa in chronic wounds of diabetic patients treated with a bioactive dressing (EGF-CMC), which consisted of a 2% carboxymethylcellulose (CMC) hydrogel loaded with epidermal growth factor (EGF). This randomized clinical trial was conducted with 25 participants: 14 treated with EGF-CMC hydrogel and 11 treated with CMC hydrogel for 12 weeks. Participants with type 2 diabetes mellitus were selected. All had diabetic foot ulcers or chronic venous ulcers. Swab collections were performed on weeks 1, 6, and 12. The laboratory analyses included the identification of strains, microbial quantification, virulence gene investigation, and the evaluation of biofilm formation. In total, 13 S. aureus strains and 15 P. aeruginosa strains were isolated. There were no statistically significant differences regarding bacterial loads and virulence genes. However, EGF-CMC-hydrogel-treated wounds were colonized by strains with lower biofilm formation abilities. The probability of isolating biofilm-producing strains from CMC-hydrogel-treated wounds was 83% greater than the probability of isolating biofilm-producing strains from EGF-CMC-treated wounds. |
format | Online Article Text |
id | pubmed-9957415 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99574152023-02-25 Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial Pessanha, Fernanda Soares de Oliveira, Beatriz Guitton Renaud Baptista Oliveira, Bianca Campos Deutsch, Gabriela Teixeira, Felipe Lopes Bokehi, Luciana Castilho Calomino, Mariana Alcântara Rodrigues de Castilho, Selma Thiré, Rossana Mara da Silva Moreira Teixeira, Lenise Arneiro de Paula, Geraldo Renato Gels Article Diabetic patients frequently develop wounds, which can be colonized by bacteria, mainly Staphylococcus aureus and Pseudomonas aeruginosa, with the ability to form biofilms. This study aimed to evaluate the colonization and biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa in chronic wounds of diabetic patients treated with a bioactive dressing (EGF-CMC), which consisted of a 2% carboxymethylcellulose (CMC) hydrogel loaded with epidermal growth factor (EGF). This randomized clinical trial was conducted with 25 participants: 14 treated with EGF-CMC hydrogel and 11 treated with CMC hydrogel for 12 weeks. Participants with type 2 diabetes mellitus were selected. All had diabetic foot ulcers or chronic venous ulcers. Swab collections were performed on weeks 1, 6, and 12. The laboratory analyses included the identification of strains, microbial quantification, virulence gene investigation, and the evaluation of biofilm formation. In total, 13 S. aureus strains and 15 P. aeruginosa strains were isolated. There were no statistically significant differences regarding bacterial loads and virulence genes. However, EGF-CMC-hydrogel-treated wounds were colonized by strains with lower biofilm formation abilities. The probability of isolating biofilm-producing strains from CMC-hydrogel-treated wounds was 83% greater than the probability of isolating biofilm-producing strains from EGF-CMC-treated wounds. MDPI 2023-02-01 /pmc/articles/PMC9957415/ /pubmed/36826286 http://dx.doi.org/10.3390/gels9020117 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pessanha, Fernanda Soares de Oliveira, Beatriz Guitton Renaud Baptista Oliveira, Bianca Campos Deutsch, Gabriela Teixeira, Felipe Lopes Bokehi, Luciana Castilho Calomino, Mariana Alcântara Rodrigues de Castilho, Selma Thiré, Rossana Mara da Silva Moreira Teixeira, Lenise Arneiro de Paula, Geraldo Renato Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial |
title | Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial |
title_full | Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial |
title_fullStr | Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial |
title_full_unstemmed | Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial |
title_short | Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial |
title_sort | effectiveness of epidermal growth factor loaded carboxymethylcellulose (egf-cmc) hydrogel in biofilm formation in wounds of diabetic patients: a randomized clinical trial |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9957415/ https://www.ncbi.nlm.nih.gov/pubmed/36826286 http://dx.doi.org/10.3390/gels9020117 |
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