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Association of GSTTI, M1 and Polymorphism in GSTPI with Chronic Periodontal Disease in a Pakistani Population
Objective: Chronic periodontal disease (CP) is a multifactorial infectious and inflammatory disease that occurs due to the challenge between the immune response of the host and specific periodontal bacteria, and that can lead to tooth loss due to damage inflicted to the supporting tissue. The curren...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9957474/ https://www.ncbi.nlm.nih.gov/pubmed/36833382 http://dx.doi.org/10.3390/genes14020455 |
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author | Arshad, Kainat Ishfaq, Uzma Asif, Muhammad Akbar, Atif Pitafi, Kehkashan Fatima Mulghani, Muhammad Rehan Shaheen, Uzman Saeed, Suleman Arif, Muhammad Bashir, Ahsan Farooq, Muhammad Brook, Alan Henry Iqbal, Furhan |
author_facet | Arshad, Kainat Ishfaq, Uzma Asif, Muhammad Akbar, Atif Pitafi, Kehkashan Fatima Mulghani, Muhammad Rehan Shaheen, Uzman Saeed, Suleman Arif, Muhammad Bashir, Ahsan Farooq, Muhammad Brook, Alan Henry Iqbal, Furhan |
author_sort | Arshad, Kainat |
collection | PubMed |
description | Objective: Chronic periodontal disease (CP) is a multifactorial infectious and inflammatory disease that occurs due to the challenge between the immune response of the host and specific periodontal bacteria, and that can lead to tooth loss due to damage inflicted to the supporting tissue. The current study investigates the genotypes of the GSTM1 and GSTT1 genes, along with the allelic frequency of the single nucleotide polymorphism [SNP; rs1695] in the GSTP1 gene and correlates them individually or in various combinations with the incidence of CP. Methods: A total of 203 clinically confirmed CP patients and 201 control subjects were enrolled from Multan and Dera Ghazi Khan Districts in Pakistan from April to July 2022. Multiplex Polymerase Chain Reaction (PCR) and tetra-primer amplification refractory mutation system–polymerase chain reaction (T-ARMS–PCR) approaches were applied to determine the genotypes of the studied GSTs. The association of rs1695 in GSTP1 with CP was studied both individually and in various combinations with GSTM1 and T1. Results: The absence of GSTM1, the presence of GSTT1 and the presence of the mutant allele (G) at rs1695 in GSTP1 were found to be significantly associated with CP. Patients aged between 10 and 30 years were more affected by CP. Conclusion: Our results indicate that the genotypes of the analyzed GSTs affect the levels of protection from oxidative stress and may therefore influence the disease progression in CP. |
format | Online Article Text |
id | pubmed-9957474 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99574742023-02-25 Association of GSTTI, M1 and Polymorphism in GSTPI with Chronic Periodontal Disease in a Pakistani Population Arshad, Kainat Ishfaq, Uzma Asif, Muhammad Akbar, Atif Pitafi, Kehkashan Fatima Mulghani, Muhammad Rehan Shaheen, Uzman Saeed, Suleman Arif, Muhammad Bashir, Ahsan Farooq, Muhammad Brook, Alan Henry Iqbal, Furhan Genes (Basel) Article Objective: Chronic periodontal disease (CP) is a multifactorial infectious and inflammatory disease that occurs due to the challenge between the immune response of the host and specific periodontal bacteria, and that can lead to tooth loss due to damage inflicted to the supporting tissue. The current study investigates the genotypes of the GSTM1 and GSTT1 genes, along with the allelic frequency of the single nucleotide polymorphism [SNP; rs1695] in the GSTP1 gene and correlates them individually or in various combinations with the incidence of CP. Methods: A total of 203 clinically confirmed CP patients and 201 control subjects were enrolled from Multan and Dera Ghazi Khan Districts in Pakistan from April to July 2022. Multiplex Polymerase Chain Reaction (PCR) and tetra-primer amplification refractory mutation system–polymerase chain reaction (T-ARMS–PCR) approaches were applied to determine the genotypes of the studied GSTs. The association of rs1695 in GSTP1 with CP was studied both individually and in various combinations with GSTM1 and T1. Results: The absence of GSTM1, the presence of GSTT1 and the presence of the mutant allele (G) at rs1695 in GSTP1 were found to be significantly associated with CP. Patients aged between 10 and 30 years were more affected by CP. Conclusion: Our results indicate that the genotypes of the analyzed GSTs affect the levels of protection from oxidative stress and may therefore influence the disease progression in CP. MDPI 2023-02-10 /pmc/articles/PMC9957474/ /pubmed/36833382 http://dx.doi.org/10.3390/genes14020455 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Arshad, Kainat Ishfaq, Uzma Asif, Muhammad Akbar, Atif Pitafi, Kehkashan Fatima Mulghani, Muhammad Rehan Shaheen, Uzman Saeed, Suleman Arif, Muhammad Bashir, Ahsan Farooq, Muhammad Brook, Alan Henry Iqbal, Furhan Association of GSTTI, M1 and Polymorphism in GSTPI with Chronic Periodontal Disease in a Pakistani Population |
title | Association of GSTTI, M1 and Polymorphism in GSTPI with Chronic Periodontal Disease in a Pakistani Population |
title_full | Association of GSTTI, M1 and Polymorphism in GSTPI with Chronic Periodontal Disease in a Pakistani Population |
title_fullStr | Association of GSTTI, M1 and Polymorphism in GSTPI with Chronic Periodontal Disease in a Pakistani Population |
title_full_unstemmed | Association of GSTTI, M1 and Polymorphism in GSTPI with Chronic Periodontal Disease in a Pakistani Population |
title_short | Association of GSTTI, M1 and Polymorphism in GSTPI with Chronic Periodontal Disease in a Pakistani Population |
title_sort | association of gstti, m1 and polymorphism in gstpi with chronic periodontal disease in a pakistani population |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9957474/ https://www.ncbi.nlm.nih.gov/pubmed/36833382 http://dx.doi.org/10.3390/genes14020455 |
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