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Association of GSTTI, M1 and Polymorphism in GSTPI with Chronic Periodontal Disease in a Pakistani Population

Objective: Chronic periodontal disease (CP) is a multifactorial infectious and inflammatory disease that occurs due to the challenge between the immune response of the host and specific periodontal bacteria, and that can lead to tooth loss due to damage inflicted to the supporting tissue. The curren...

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Autores principales: Arshad, Kainat, Ishfaq, Uzma, Asif, Muhammad, Akbar, Atif, Pitafi, Kehkashan Fatima, Mulghani, Muhammad Rehan, Shaheen, Uzman, Saeed, Suleman, Arif, Muhammad, Bashir, Ahsan, Farooq, Muhammad, Brook, Alan Henry, Iqbal, Furhan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9957474/
https://www.ncbi.nlm.nih.gov/pubmed/36833382
http://dx.doi.org/10.3390/genes14020455
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author Arshad, Kainat
Ishfaq, Uzma
Asif, Muhammad
Akbar, Atif
Pitafi, Kehkashan Fatima
Mulghani, Muhammad Rehan
Shaheen, Uzman
Saeed, Suleman
Arif, Muhammad
Bashir, Ahsan
Farooq, Muhammad
Brook, Alan Henry
Iqbal, Furhan
author_facet Arshad, Kainat
Ishfaq, Uzma
Asif, Muhammad
Akbar, Atif
Pitafi, Kehkashan Fatima
Mulghani, Muhammad Rehan
Shaheen, Uzman
Saeed, Suleman
Arif, Muhammad
Bashir, Ahsan
Farooq, Muhammad
Brook, Alan Henry
Iqbal, Furhan
author_sort Arshad, Kainat
collection PubMed
description Objective: Chronic periodontal disease (CP) is a multifactorial infectious and inflammatory disease that occurs due to the challenge between the immune response of the host and specific periodontal bacteria, and that can lead to tooth loss due to damage inflicted to the supporting tissue. The current study investigates the genotypes of the GSTM1 and GSTT1 genes, along with the allelic frequency of the single nucleotide polymorphism [SNP; rs1695] in the GSTP1 gene and correlates them individually or in various combinations with the incidence of CP. Methods: A total of 203 clinically confirmed CP patients and 201 control subjects were enrolled from Multan and Dera Ghazi Khan Districts in Pakistan from April to July 2022. Multiplex Polymerase Chain Reaction (PCR) and tetra-primer amplification refractory mutation system–polymerase chain reaction (T-ARMS–PCR) approaches were applied to determine the genotypes of the studied GSTs. The association of rs1695 in GSTP1 with CP was studied both individually and in various combinations with GSTM1 and T1. Results: The absence of GSTM1, the presence of GSTT1 and the presence of the mutant allele (G) at rs1695 in GSTP1 were found to be significantly associated with CP. Patients aged between 10 and 30 years were more affected by CP. Conclusion: Our results indicate that the genotypes of the analyzed GSTs affect the levels of protection from oxidative stress and may therefore influence the disease progression in CP.
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spelling pubmed-99574742023-02-25 Association of GSTTI, M1 and Polymorphism in GSTPI with Chronic Periodontal Disease in a Pakistani Population Arshad, Kainat Ishfaq, Uzma Asif, Muhammad Akbar, Atif Pitafi, Kehkashan Fatima Mulghani, Muhammad Rehan Shaheen, Uzman Saeed, Suleman Arif, Muhammad Bashir, Ahsan Farooq, Muhammad Brook, Alan Henry Iqbal, Furhan Genes (Basel) Article Objective: Chronic periodontal disease (CP) is a multifactorial infectious and inflammatory disease that occurs due to the challenge between the immune response of the host and specific periodontal bacteria, and that can lead to tooth loss due to damage inflicted to the supporting tissue. The current study investigates the genotypes of the GSTM1 and GSTT1 genes, along with the allelic frequency of the single nucleotide polymorphism [SNP; rs1695] in the GSTP1 gene and correlates them individually or in various combinations with the incidence of CP. Methods: A total of 203 clinically confirmed CP patients and 201 control subjects were enrolled from Multan and Dera Ghazi Khan Districts in Pakistan from April to July 2022. Multiplex Polymerase Chain Reaction (PCR) and tetra-primer amplification refractory mutation system–polymerase chain reaction (T-ARMS–PCR) approaches were applied to determine the genotypes of the studied GSTs. The association of rs1695 in GSTP1 with CP was studied both individually and in various combinations with GSTM1 and T1. Results: The absence of GSTM1, the presence of GSTT1 and the presence of the mutant allele (G) at rs1695 in GSTP1 were found to be significantly associated with CP. Patients aged between 10 and 30 years were more affected by CP. Conclusion: Our results indicate that the genotypes of the analyzed GSTs affect the levels of protection from oxidative stress and may therefore influence the disease progression in CP. MDPI 2023-02-10 /pmc/articles/PMC9957474/ /pubmed/36833382 http://dx.doi.org/10.3390/genes14020455 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Arshad, Kainat
Ishfaq, Uzma
Asif, Muhammad
Akbar, Atif
Pitafi, Kehkashan Fatima
Mulghani, Muhammad Rehan
Shaheen, Uzman
Saeed, Suleman
Arif, Muhammad
Bashir, Ahsan
Farooq, Muhammad
Brook, Alan Henry
Iqbal, Furhan
Association of GSTTI, M1 and Polymorphism in GSTPI with Chronic Periodontal Disease in a Pakistani Population
title Association of GSTTI, M1 and Polymorphism in GSTPI with Chronic Periodontal Disease in a Pakistani Population
title_full Association of GSTTI, M1 and Polymorphism in GSTPI with Chronic Periodontal Disease in a Pakistani Population
title_fullStr Association of GSTTI, M1 and Polymorphism in GSTPI with Chronic Periodontal Disease in a Pakistani Population
title_full_unstemmed Association of GSTTI, M1 and Polymorphism in GSTPI with Chronic Periodontal Disease in a Pakistani Population
title_short Association of GSTTI, M1 and Polymorphism in GSTPI with Chronic Periodontal Disease in a Pakistani Population
title_sort association of gstti, m1 and polymorphism in gstpi with chronic periodontal disease in a pakistani population
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9957474/
https://www.ncbi.nlm.nih.gov/pubmed/36833382
http://dx.doi.org/10.3390/genes14020455
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