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Diversity and Distribution Characteristics of Viruses from Soda Lakes

Viruses are the most abundant living things and a source of genetic variation. Despite recent research, we know little about their biodiversity and geographic distribution. We used different bioinformatics tools, MG-RAST, genome detective web tools, and GenomeVx, to describe the first metagenomic ex...

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Autores principales: ZeinEldin, Ramadan A., Ahmed, Marwa M., Hassanein, Wael S., Elshafey, Naglaa, Sofy, Ahmed R., Hamedo, Hend A., Elnosary, Mohamed E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9957498/
https://www.ncbi.nlm.nih.gov/pubmed/36833250
http://dx.doi.org/10.3390/genes14020323
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author ZeinEldin, Ramadan A.
Ahmed, Marwa M.
Hassanein, Wael S.
Elshafey, Naglaa
Sofy, Ahmed R.
Hamedo, Hend A.
Elnosary, Mohamed E.
author_facet ZeinEldin, Ramadan A.
Ahmed, Marwa M.
Hassanein, Wael S.
Elshafey, Naglaa
Sofy, Ahmed R.
Hamedo, Hend A.
Elnosary, Mohamed E.
author_sort ZeinEldin, Ramadan A.
collection PubMed
description Viruses are the most abundant living things and a source of genetic variation. Despite recent research, we know little about their biodiversity and geographic distribution. We used different bioinformatics tools, MG-RAST, genome detective web tools, and GenomeVx, to describe the first metagenomic examination of haloviruses in Wadi Al-Natrun. The discovered viromes had remarkably different taxonomic compositions. Most sequences were derived from double-stranded DNA viruses, especially from Myoviridae, Podoviridae, Siphoviridae, Herpesviridae, Bicaudaviridae, and Phycodnaviridae families; single-stranded DNA viruses, especially from the family Microviridae; and positive-strand RNA viruses, especially from the family Potyviridae. Additionally, our results showed that Myohalovirus chaoS9 has eight Contigs and is annotated to 18 proteins as follows: tail sheath protein, tco, nep, five uncharacterized proteins, HCO, major capsid protein, putative pro head protease protein, putative head assembly protein, CxxC motive protein, terl, HTH domain protein, and terS Exon 2. Additionally, Halorubrum phage CGphi46 has 19 proteins in the brine sample as follows: portal protein, 17 hypothetical proteins, major capsid protein, etc. This study reveals viral lineages, suggesting the Virus’s global dispersal more than other microorganisms. Our study clarifies how viral communities are connected and how the global environment changes.
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spelling pubmed-99574982023-02-25 Diversity and Distribution Characteristics of Viruses from Soda Lakes ZeinEldin, Ramadan A. Ahmed, Marwa M. Hassanein, Wael S. Elshafey, Naglaa Sofy, Ahmed R. Hamedo, Hend A. Elnosary, Mohamed E. Genes (Basel) Article Viruses are the most abundant living things and a source of genetic variation. Despite recent research, we know little about their biodiversity and geographic distribution. We used different bioinformatics tools, MG-RAST, genome detective web tools, and GenomeVx, to describe the first metagenomic examination of haloviruses in Wadi Al-Natrun. The discovered viromes had remarkably different taxonomic compositions. Most sequences were derived from double-stranded DNA viruses, especially from Myoviridae, Podoviridae, Siphoviridae, Herpesviridae, Bicaudaviridae, and Phycodnaviridae families; single-stranded DNA viruses, especially from the family Microviridae; and positive-strand RNA viruses, especially from the family Potyviridae. Additionally, our results showed that Myohalovirus chaoS9 has eight Contigs and is annotated to 18 proteins as follows: tail sheath protein, tco, nep, five uncharacterized proteins, HCO, major capsid protein, putative pro head protease protein, putative head assembly protein, CxxC motive protein, terl, HTH domain protein, and terS Exon 2. Additionally, Halorubrum phage CGphi46 has 19 proteins in the brine sample as follows: portal protein, 17 hypothetical proteins, major capsid protein, etc. This study reveals viral lineages, suggesting the Virus’s global dispersal more than other microorganisms. Our study clarifies how viral communities are connected and how the global environment changes. MDPI 2023-01-26 /pmc/articles/PMC9957498/ /pubmed/36833250 http://dx.doi.org/10.3390/genes14020323 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
ZeinEldin, Ramadan A.
Ahmed, Marwa M.
Hassanein, Wael S.
Elshafey, Naglaa
Sofy, Ahmed R.
Hamedo, Hend A.
Elnosary, Mohamed E.
Diversity and Distribution Characteristics of Viruses from Soda Lakes
title Diversity and Distribution Characteristics of Viruses from Soda Lakes
title_full Diversity and Distribution Characteristics of Viruses from Soda Lakes
title_fullStr Diversity and Distribution Characteristics of Viruses from Soda Lakes
title_full_unstemmed Diversity and Distribution Characteristics of Viruses from Soda Lakes
title_short Diversity and Distribution Characteristics of Viruses from Soda Lakes
title_sort diversity and distribution characteristics of viruses from soda lakes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9957498/
https://www.ncbi.nlm.nih.gov/pubmed/36833250
http://dx.doi.org/10.3390/genes14020323
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