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A prediction model for prognosis of gastric adenocarcinoma based on six metabolism-related genes
BACKGROUND: The study of tumor metabolism is of great value to elucidate the mechanism of tumorigenesis and predict the prognosis of patients. However, the prognostic role of metabolism-related genes (MRGs) in gastric adenocarcinoma (GAD) remains poorly understood. METHODS: We downloaded the gene ch...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9957706/ https://www.ncbi.nlm.nih.gov/pubmed/36852096 http://dx.doi.org/10.1016/j.bbrep.2023.101440 |
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author | Zhao, Jingyu Liu, Yu Cui, Qianwen He, Rongli Zhao, Jia-Rong Lu, Li Wang, Hong-Qiang Dai, Haiming Wang, Hongzhi Yang, Wulin |
author_facet | Zhao, Jingyu Liu, Yu Cui, Qianwen He, Rongli Zhao, Jia-Rong Lu, Li Wang, Hong-Qiang Dai, Haiming Wang, Hongzhi Yang, Wulin |
author_sort | Zhao, Jingyu |
collection | PubMed |
description | BACKGROUND: The study of tumor metabolism is of great value to elucidate the mechanism of tumorigenesis and predict the prognosis of patients. However, the prognostic role of metabolism-related genes (MRGs) in gastric adenocarcinoma (GAD) remains poorly understood. METHODS: We downloaded the gene chip dataset GSE79973 (n = 20) of GAD from the Gene Expression Omnibus (GEO) database to compare differentially expressed genes (DEGs) between normal and tumor tissues. We then extracted MRGs from these DEGs and systematically investigated the prognostic value of these differential MRGs for predicting patients' overall survival by univariable and multivariable Cox regression analysis. Six metabolic genes (ACOX3, APOE, DIO2, HSD17B4, NUAK1, and WHSC1L1) were identified as prognosis-associated hub genes, which were used to build a prognostic model in the training dataset GSE15459 (n = 200), and then validated in the dataset GSE62254 (n = 300). RESULTS: Patients were divided into high-risk and low-risk subgroups based on the model's risk score, and it was found that patients in the high-risk subgroup had shorter overall survival than those in the low-risk subgroup, both in the training and testing datasets. In addition, for the training and testing cohorts, the area under the ROC curve of the prognostic model for one-year survival prediction was 0.723 and 0.667, respectively, indicating that the model has good predictive performance. Furthermore, we established a nomogram based on tumor stage and risk score to effectively predict the overall survival (OS) of GAD patients. The expression of 6 MRGs at the protein level was confirmed by immunohistochemistry (IHC). Kaplan-Meier survival analysis further confirmed that their expression influenced OS in GAD patients. CONCLUSION: Collectively, the 6 MRGs signature might be a reliable tool for assessing OS in GAD patients, with potential application value in clinical decision-making and individualized therapy. |
format | Online Article Text |
id | pubmed-9957706 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-99577062023-02-26 A prediction model for prognosis of gastric adenocarcinoma based on six metabolism-related genes Zhao, Jingyu Liu, Yu Cui, Qianwen He, Rongli Zhao, Jia-Rong Lu, Li Wang, Hong-Qiang Dai, Haiming Wang, Hongzhi Yang, Wulin Biochem Biophys Rep Research Article BACKGROUND: The study of tumor metabolism is of great value to elucidate the mechanism of tumorigenesis and predict the prognosis of patients. However, the prognostic role of metabolism-related genes (MRGs) in gastric adenocarcinoma (GAD) remains poorly understood. METHODS: We downloaded the gene chip dataset GSE79973 (n = 20) of GAD from the Gene Expression Omnibus (GEO) database to compare differentially expressed genes (DEGs) between normal and tumor tissues. We then extracted MRGs from these DEGs and systematically investigated the prognostic value of these differential MRGs for predicting patients' overall survival by univariable and multivariable Cox regression analysis. Six metabolic genes (ACOX3, APOE, DIO2, HSD17B4, NUAK1, and WHSC1L1) were identified as prognosis-associated hub genes, which were used to build a prognostic model in the training dataset GSE15459 (n = 200), and then validated in the dataset GSE62254 (n = 300). RESULTS: Patients were divided into high-risk and low-risk subgroups based on the model's risk score, and it was found that patients in the high-risk subgroup had shorter overall survival than those in the low-risk subgroup, both in the training and testing datasets. In addition, for the training and testing cohorts, the area under the ROC curve of the prognostic model for one-year survival prediction was 0.723 and 0.667, respectively, indicating that the model has good predictive performance. Furthermore, we established a nomogram based on tumor stage and risk score to effectively predict the overall survival (OS) of GAD patients. The expression of 6 MRGs at the protein level was confirmed by immunohistochemistry (IHC). Kaplan-Meier survival analysis further confirmed that their expression influenced OS in GAD patients. CONCLUSION: Collectively, the 6 MRGs signature might be a reliable tool for assessing OS in GAD patients, with potential application value in clinical decision-making and individualized therapy. Elsevier 2023-02-18 /pmc/articles/PMC9957706/ /pubmed/36852096 http://dx.doi.org/10.1016/j.bbrep.2023.101440 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Zhao, Jingyu Liu, Yu Cui, Qianwen He, Rongli Zhao, Jia-Rong Lu, Li Wang, Hong-Qiang Dai, Haiming Wang, Hongzhi Yang, Wulin A prediction model for prognosis of gastric adenocarcinoma based on six metabolism-related genes |
title | A prediction model for prognosis of gastric adenocarcinoma based on six metabolism-related genes |
title_full | A prediction model for prognosis of gastric adenocarcinoma based on six metabolism-related genes |
title_fullStr | A prediction model for prognosis of gastric adenocarcinoma based on six metabolism-related genes |
title_full_unstemmed | A prediction model for prognosis of gastric adenocarcinoma based on six metabolism-related genes |
title_short | A prediction model for prognosis of gastric adenocarcinoma based on six metabolism-related genes |
title_sort | prediction model for prognosis of gastric adenocarcinoma based on six metabolism-related genes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9957706/ https://www.ncbi.nlm.nih.gov/pubmed/36852096 http://dx.doi.org/10.1016/j.bbrep.2023.101440 |
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