Protocol to characterize G(i/o) and G(s) protein-coupled receptors in transiently transfected cells using ELISA and cAMP measurements

Activation of G(s) or G(i/o) protein-coupled receptors (GPCRs) leads to changes of intracellular cyclic adenosine monophosphate (cAMP) levels. This protocol describes steps for cloning HA- and FLAG-tagged GPCRs, transient transfection of CHO-K1 or HEK293-T cells, and determination of basal and ligan...

Descripción completa

Detalles Bibliográficos
Autores principales: Liebing, Aenne-Dorothea, Krumbholz, Petra, Stäubert, Claudia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958081/
https://www.ncbi.nlm.nih.gov/pubmed/36853674
http://dx.doi.org/10.1016/j.xpro.2023.102120

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958081/
https://www.ncbi.nlm.nih.gov/pubmed/36853674
http://dx.doi.org/10.1016/j.xpro.2023.102120

Ejemplares similares