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Protocol to characterize G(i/o) and G(s) protein-coupled receptors in transiently transfected cells using ELISA and cAMP measurements

Activation of G(s) or G(i/o) protein-coupled receptors (GPCRs) leads to changes of intracellular cyclic adenosine monophosphate (cAMP) levels. This protocol describes steps for cloning HA- and FLAG-tagged GPCRs, transient transfection of CHO-K1 or HEK293-T cells, and determination of basal and ligan...

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Detalles Bibliográficos
Autores principales:	Liebing, Aenne-Dorothea, Krumbholz, Petra, Stäubert, Claudia
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958081/ https://www.ncbi.nlm.nih.gov/pubmed/36853674 http://dx.doi.org/10.1016/j.xpro.2023.102120

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