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Flow cytometry and immunohistochemistry of the mouse dural meninges for immunological and virological assessments

The highly vascularized meninges protect the surface of the central nervous system and contain a dense network of immune cells controlling neuroinfection and neuroinflammation. Here, we present techniques for the immunological and virological assessment of mouse dural meninges. We describe steps for...

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Autores principales: Roussel-Queval, Annie, Rebejac, Julie, Eme-Scolan, Elisa, Paroutaud, Laurie Arnaud, Rua, Rejane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958090/
https://www.ncbi.nlm.nih.gov/pubmed/36853673
http://dx.doi.org/10.1016/j.xpro.2023.102119
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author Roussel-Queval, Annie
Rebejac, Julie
Eme-Scolan, Elisa
Paroutaud, Laurie Arnaud
Rua, Rejane
author_facet Roussel-Queval, Annie
Rebejac, Julie
Eme-Scolan, Elisa
Paroutaud, Laurie Arnaud
Rua, Rejane
author_sort Roussel-Queval, Annie
collection PubMed
description The highly vascularized meninges protect the surface of the central nervous system and contain a dense network of immune cells controlling neuroinfection and neuroinflammation. Here, we present techniques for the immunological and virological assessment of mouse dural meninges. We describe steps for immunophenotyping including meninges extraction and digestion, immunostaining, and flow cytometry. We then describe viral assessment upon lymphocytic choriomeningitis virus infection including steps for fixation of the meninges in the skull, whole-mount immunohistochemistry, and confocal imaging. For complete details on the use and execution of this protocol, please refer to Rebejac et al. (2022).(1)
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spelling pubmed-99580902023-02-26 Flow cytometry and immunohistochemistry of the mouse dural meninges for immunological and virological assessments Roussel-Queval, Annie Rebejac, Julie Eme-Scolan, Elisa Paroutaud, Laurie Arnaud Rua, Rejane STAR Protoc Protocol The highly vascularized meninges protect the surface of the central nervous system and contain a dense network of immune cells controlling neuroinfection and neuroinflammation. Here, we present techniques for the immunological and virological assessment of mouse dural meninges. We describe steps for immunophenotyping including meninges extraction and digestion, immunostaining, and flow cytometry. We then describe viral assessment upon lymphocytic choriomeningitis virus infection including steps for fixation of the meninges in the skull, whole-mount immunohistochemistry, and confocal imaging. For complete details on the use and execution of this protocol, please refer to Rebejac et al. (2022).(1) Elsevier 2023-02-14 /pmc/articles/PMC9958090/ /pubmed/36853673 http://dx.doi.org/10.1016/j.xpro.2023.102119 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Roussel-Queval, Annie
Rebejac, Julie
Eme-Scolan, Elisa
Paroutaud, Laurie Arnaud
Rua, Rejane
Flow cytometry and immunohistochemistry of the mouse dural meninges for immunological and virological assessments
title Flow cytometry and immunohistochemistry of the mouse dural meninges for immunological and virological assessments
title_full Flow cytometry and immunohistochemistry of the mouse dural meninges for immunological and virological assessments
title_fullStr Flow cytometry and immunohistochemistry of the mouse dural meninges for immunological and virological assessments
title_full_unstemmed Flow cytometry and immunohistochemistry of the mouse dural meninges for immunological and virological assessments
title_short Flow cytometry and immunohistochemistry of the mouse dural meninges for immunological and virological assessments
title_sort flow cytometry and immunohistochemistry of the mouse dural meninges for immunological and virological assessments
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958090/
https://www.ncbi.nlm.nih.gov/pubmed/36853673
http://dx.doi.org/10.1016/j.xpro.2023.102119
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