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Single-cell RNA sequencing in orthopedic research
Although previous RNA sequencing methods have been widely used in orthopedic research and have provided ideas for therapeutic strategies, the specific mechanisms of some orthopedic disorders, including osteoarthritis, lumbar disc herniation, rheumatoid arthritis, fractures, tendon injuries, spinal c...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958119/ https://www.ncbi.nlm.nih.gov/pubmed/36828839 http://dx.doi.org/10.1038/s41413-023-00245-0 |
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author | Wang, Tao Wang, Ling Zhang, Liping Long, Yubin Zhang, Yingze Hou, Zhiyong |
author_facet | Wang, Tao Wang, Ling Zhang, Liping Long, Yubin Zhang, Yingze Hou, Zhiyong |
author_sort | Wang, Tao |
collection | PubMed |
description | Although previous RNA sequencing methods have been widely used in orthopedic research and have provided ideas for therapeutic strategies, the specific mechanisms of some orthopedic disorders, including osteoarthritis, lumbar disc herniation, rheumatoid arthritis, fractures, tendon injuries, spinal cord injury, heterotopic ossification, and osteosarcoma, require further elucidation. The emergence of the single-cell RNA sequencing (scRNA-seq) technique has introduced a new era of research on these topics, as this method provides information regarding cellular heterogeneity, new cell subtypes, functions of novel subclusters, potential molecular mechanisms, cell-fate transitions, and cell‒cell interactions that are involved in the development of orthopedic diseases. Here, we summarize the cell subpopulations, genes, and underlying mechanisms involved in the development of orthopedic diseases identified by scRNA-seq, improving our understanding of the pathology of these diseases and providing new insights into therapeutic approaches. |
format | Online Article Text |
id | pubmed-9958119 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-99581192023-02-26 Single-cell RNA sequencing in orthopedic research Wang, Tao Wang, Ling Zhang, Liping Long, Yubin Zhang, Yingze Hou, Zhiyong Bone Res Review Article Although previous RNA sequencing methods have been widely used in orthopedic research and have provided ideas for therapeutic strategies, the specific mechanisms of some orthopedic disorders, including osteoarthritis, lumbar disc herniation, rheumatoid arthritis, fractures, tendon injuries, spinal cord injury, heterotopic ossification, and osteosarcoma, require further elucidation. The emergence of the single-cell RNA sequencing (scRNA-seq) technique has introduced a new era of research on these topics, as this method provides information regarding cellular heterogeneity, new cell subtypes, functions of novel subclusters, potential molecular mechanisms, cell-fate transitions, and cell‒cell interactions that are involved in the development of orthopedic diseases. Here, we summarize the cell subpopulations, genes, and underlying mechanisms involved in the development of orthopedic diseases identified by scRNA-seq, improving our understanding of the pathology of these diseases and providing new insights into therapeutic approaches. Nature Publishing Group UK 2023-02-24 /pmc/articles/PMC9958119/ /pubmed/36828839 http://dx.doi.org/10.1038/s41413-023-00245-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Review Article Wang, Tao Wang, Ling Zhang, Liping Long, Yubin Zhang, Yingze Hou, Zhiyong Single-cell RNA sequencing in orthopedic research |
title | Single-cell RNA sequencing in orthopedic research |
title_full | Single-cell RNA sequencing in orthopedic research |
title_fullStr | Single-cell RNA sequencing in orthopedic research |
title_full_unstemmed | Single-cell RNA sequencing in orthopedic research |
title_short | Single-cell RNA sequencing in orthopedic research |
title_sort | single-cell rna sequencing in orthopedic research |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958119/ https://www.ncbi.nlm.nih.gov/pubmed/36828839 http://dx.doi.org/10.1038/s41413-023-00245-0 |
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