In vivo analysis of FBXO45-mediated fibrosis and liver tumorigenesis in a chemically induced mouse model of hepatocellular carcinoma

FBXO45, an E3 ubiquitin ligase highly expressed in liver tumors, is positively correlated with poor survival of hepatocellular carcinogenesis (HCC) patients, but whether FBXO45 drives HCC tumorigenesis remains largely unclear. Here, we describe a protocol that shortens the observation period for HCC...

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Detalles Bibliográficos
Autores principales: Zhang, Jie, Lin, Xiao-Tong, Fang, Lei, Xie, Chuan-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958395/
https://www.ncbi.nlm.nih.gov/pubmed/36853700
http://dx.doi.org/10.1016/j.xpro.2023.102124
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author Zhang, Jie
Lin, Xiao-Tong
Fang, Lei
Xie, Chuan-Ming
author_facet Zhang, Jie
Lin, Xiao-Tong
Fang, Lei
Xie, Chuan-Ming
author_sort Zhang, Jie
collection PubMed
description FBXO45, an E3 ubiquitin ligase highly expressed in liver tumors, is positively correlated with poor survival of hepatocellular carcinogenesis (HCC) patients, but whether FBXO45 drives HCC tumorigenesis remains largely unclear. Here, we describe a protocol that shortens the observation period for HCC tumorigenesis to assess the effects of FBXO45 in a DEN/CCl(4)-induced HCC mouse model. We describe steps for chemical induction of HCC in FBXO45-overexpressing mice, followed by tissue collection and pathology assessment via quantitative real-time PCR, histology, and immunohistochemistry. For complete details on the use and execution of this protocol, please refer to Lin et al. (2021).(1)
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spelling pubmed-99583952023-02-26 In vivo analysis of FBXO45-mediated fibrosis and liver tumorigenesis in a chemically induced mouse model of hepatocellular carcinoma Zhang, Jie Lin, Xiao-Tong Fang, Lei Xie, Chuan-Ming STAR Protoc Protocol FBXO45, an E3 ubiquitin ligase highly expressed in liver tumors, is positively correlated with poor survival of hepatocellular carcinogenesis (HCC) patients, but whether FBXO45 drives HCC tumorigenesis remains largely unclear. Here, we describe a protocol that shortens the observation period for HCC tumorigenesis to assess the effects of FBXO45 in a DEN/CCl(4)-induced HCC mouse model. We describe steps for chemical induction of HCC in FBXO45-overexpressing mice, followed by tissue collection and pathology assessment via quantitative real-time PCR, histology, and immunohistochemistry. For complete details on the use and execution of this protocol, please refer to Lin et al. (2021).(1) Elsevier 2023-02-15 /pmc/articles/PMC9958395/ /pubmed/36853700 http://dx.doi.org/10.1016/j.xpro.2023.102124 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Zhang, Jie
Lin, Xiao-Tong
Fang, Lei
Xie, Chuan-Ming
In vivo analysis of FBXO45-mediated fibrosis and liver tumorigenesis in a chemically induced mouse model of hepatocellular carcinoma
title In vivo analysis of FBXO45-mediated fibrosis and liver tumorigenesis in a chemically induced mouse model of hepatocellular carcinoma
title_full In vivo analysis of FBXO45-mediated fibrosis and liver tumorigenesis in a chemically induced mouse model of hepatocellular carcinoma
title_fullStr In vivo analysis of FBXO45-mediated fibrosis and liver tumorigenesis in a chemically induced mouse model of hepatocellular carcinoma
title_full_unstemmed In vivo analysis of FBXO45-mediated fibrosis and liver tumorigenesis in a chemically induced mouse model of hepatocellular carcinoma
title_short In vivo analysis of FBXO45-mediated fibrosis and liver tumorigenesis in a chemically induced mouse model of hepatocellular carcinoma
title_sort in vivo analysis of fbxo45-mediated fibrosis and liver tumorigenesis in a chemically induced mouse model of hepatocellular carcinoma
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958395/
https://www.ncbi.nlm.nih.gov/pubmed/36853700
http://dx.doi.org/10.1016/j.xpro.2023.102124
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