Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia mice

Practical procedures for sorting and analysis of leukemia stem cells (LSCs) are to improve our understanding of chronic myelogenous leukemia (CML). Here, we present a detailed magnetic-bead-based sorting and flow-cytometry-based analysis protocol for LSCs in BCR-ABL-driven CML mice. We describe step...

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Detalles Bibliográficos
Autores principales: Liu, Chang, Lin, Xiaoying, Jin, Yanli, Pan, Jingxuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958397/
https://www.ncbi.nlm.nih.gov/pubmed/36853670
http://dx.doi.org/10.1016/j.xpro.2023.102123
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author Liu, Chang
Lin, Xiaoying
Jin, Yanli
Pan, Jingxuan
author_facet Liu, Chang
Lin, Xiaoying
Jin, Yanli
Pan, Jingxuan
author_sort Liu, Chang
collection PubMed
description Practical procedures for sorting and analysis of leukemia stem cells (LSCs) are to improve our understanding of chronic myelogenous leukemia (CML). Here, we present a detailed magnetic-bead-based sorting and flow-cytometry-based analysis protocol for LSCs in BCR-ABL-driven CML mice. We describe steps for sorting and functional analysis of BCR-ABL-expressing c-Kit(+) cells (GFP(+)c-Kit(+)) from CML mice as well as antibody staining and gating strategies for characterization of leukemia stem/progenitor cells and myeloid leukemia cells. For complete details on the use and execution of this protocol, please refer to Liu et al. (2022).(1)
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spelling pubmed-99583972023-02-26 Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia mice Liu, Chang Lin, Xiaoying Jin, Yanli Pan, Jingxuan STAR Protoc Protocol Practical procedures for sorting and analysis of leukemia stem cells (LSCs) are to improve our understanding of chronic myelogenous leukemia (CML). Here, we present a detailed magnetic-bead-based sorting and flow-cytometry-based analysis protocol for LSCs in BCR-ABL-driven CML mice. We describe steps for sorting and functional analysis of BCR-ABL-expressing c-Kit(+) cells (GFP(+)c-Kit(+)) from CML mice as well as antibody staining and gating strategies for characterization of leukemia stem/progenitor cells and myeloid leukemia cells. For complete details on the use and execution of this protocol, please refer to Liu et al. (2022).(1) Elsevier 2023-02-15 /pmc/articles/PMC9958397/ /pubmed/36853670 http://dx.doi.org/10.1016/j.xpro.2023.102123 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Liu, Chang
Lin, Xiaoying
Jin, Yanli
Pan, Jingxuan
Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia mice
title Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia mice
title_full Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia mice
title_fullStr Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia mice
title_full_unstemmed Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia mice
title_short Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia mice
title_sort protocol for isolation and analysis of the leukemia stem cells in bcr-abl-driven chronic myelogenous leukemia mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958397/
https://www.ncbi.nlm.nih.gov/pubmed/36853670
http://dx.doi.org/10.1016/j.xpro.2023.102123
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