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Biomechanical Assessment of Red Blood Cells in Pulsatile Blood Flows

As rheological properties are substantially influenced by red blood cells (RBCs) and plasma, the separation of their individual contributions in blood is essential. The estimation of multiple rheological factors is a critical issue for effective early detection of diseases. In this study, three rheo...

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Autor principal: Kang, Yang Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958583/
https://www.ncbi.nlm.nih.gov/pubmed/36838017
http://dx.doi.org/10.3390/mi14020317
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author Kang, Yang Jun
author_facet Kang, Yang Jun
author_sort Kang, Yang Jun
collection PubMed
description As rheological properties are substantially influenced by red blood cells (RBCs) and plasma, the separation of their individual contributions in blood is essential. The estimation of multiple rheological factors is a critical issue for effective early detection of diseases. In this study, three rheological properties (i.e., viscoelasticity, RBC aggregation, and blood junction pressure) are measured by analyzing the blood velocity and image intensity in a microfluidic device. Using a single syringe pump, the blood flow rate sets to a pulsatile flow pattern (Q(b)[t] = 1 + 0.5 sin(2πt/240) mL/h). Based on the discrete fluidic circuit model, the analytical formula of the time constant (λ(b)) as viscoelasticity is derived and obtained at specific time intervals by analyzing the pulsatile blood velocity. To obtain RBC aggregation by reducing blood velocity substantially, an air compliance unit (ACU) is used to connect polyethylene tubing (i.d. = 250 µm, length = 150 mm) to the blood channel in parallel. The RBC aggregation index (AI) is obtained by analyzing the microscopic image intensity. The blood junction pressure (β) is obtained by integrating the blood velocity within the ACU. As a demonstration, the present method is then applied to detect either RBC-aggregated blood with different concentrations of dextran solution or hardened blood with thermally shocked RBCs. Thus, it can be concluded that the present method has the ability to consistently detect differences in diluent or RBCs in terms of three rheological properties.
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spelling pubmed-99585832023-02-26 Biomechanical Assessment of Red Blood Cells in Pulsatile Blood Flows Kang, Yang Jun Micromachines (Basel) Article As rheological properties are substantially influenced by red blood cells (RBCs) and plasma, the separation of their individual contributions in blood is essential. The estimation of multiple rheological factors is a critical issue for effective early detection of diseases. In this study, three rheological properties (i.e., viscoelasticity, RBC aggregation, and blood junction pressure) are measured by analyzing the blood velocity and image intensity in a microfluidic device. Using a single syringe pump, the blood flow rate sets to a pulsatile flow pattern (Q(b)[t] = 1 + 0.5 sin(2πt/240) mL/h). Based on the discrete fluidic circuit model, the analytical formula of the time constant (λ(b)) as viscoelasticity is derived and obtained at specific time intervals by analyzing the pulsatile blood velocity. To obtain RBC aggregation by reducing blood velocity substantially, an air compliance unit (ACU) is used to connect polyethylene tubing (i.d. = 250 µm, length = 150 mm) to the blood channel in parallel. The RBC aggregation index (AI) is obtained by analyzing the microscopic image intensity. The blood junction pressure (β) is obtained by integrating the blood velocity within the ACU. As a demonstration, the present method is then applied to detect either RBC-aggregated blood with different concentrations of dextran solution or hardened blood with thermally shocked RBCs. Thus, it can be concluded that the present method has the ability to consistently detect differences in diluent or RBCs in terms of three rheological properties. MDPI 2023-01-26 /pmc/articles/PMC9958583/ /pubmed/36838017 http://dx.doi.org/10.3390/mi14020317 Text en © 2023 by the author. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kang, Yang Jun
Biomechanical Assessment of Red Blood Cells in Pulsatile Blood Flows
title Biomechanical Assessment of Red Blood Cells in Pulsatile Blood Flows
title_full Biomechanical Assessment of Red Blood Cells in Pulsatile Blood Flows
title_fullStr Biomechanical Assessment of Red Blood Cells in Pulsatile Blood Flows
title_full_unstemmed Biomechanical Assessment of Red Blood Cells in Pulsatile Blood Flows
title_short Biomechanical Assessment of Red Blood Cells in Pulsatile Blood Flows
title_sort biomechanical assessment of red blood cells in pulsatile blood flows
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958583/
https://www.ncbi.nlm.nih.gov/pubmed/36838017
http://dx.doi.org/10.3390/mi14020317
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