Cargando…

Association of Circulating Neutrophils with Relative Volume of Lipid-Rich Necrotic Core of Coronary Plaques in Stable Patients: A Substudy of SMARTool European Project

Background and Aims: Coronary atherosclerosis is a chronic non-resolving inflammatory process wherein the interaction of innate immune cells and platelets plays a major role. Circulating neutrophils, in particular, adhere to the activated endothelium and migrate into the vascular wall, promoting mon...

Descripción completa

Detalles Bibliográficos
Autores principales: Sbrana, Silverio, Cecchettini, Antonella, Bastiani, Luca, Mazzone, Annamaria, Vozzi, Federico, Caselli, Chiara, Neglia, Danilo, Clemente, Alberto, Scholte, Arthur J. H. A., Parodi, Oberdan, Pelosi, Gualtiero, Rocchiccioli, Silvia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958623/
https://www.ncbi.nlm.nih.gov/pubmed/36836785
http://dx.doi.org/10.3390/life13020428
_version_ 1784895070092132352
author Sbrana, Silverio
Cecchettini, Antonella
Bastiani, Luca
Mazzone, Annamaria
Vozzi, Federico
Caselli, Chiara
Neglia, Danilo
Clemente, Alberto
Scholte, Arthur J. H. A.
Parodi, Oberdan
Pelosi, Gualtiero
Rocchiccioli, Silvia
author_facet Sbrana, Silverio
Cecchettini, Antonella
Bastiani, Luca
Mazzone, Annamaria
Vozzi, Federico
Caselli, Chiara
Neglia, Danilo
Clemente, Alberto
Scholte, Arthur J. H. A.
Parodi, Oberdan
Pelosi, Gualtiero
Rocchiccioli, Silvia
author_sort Sbrana, Silverio
collection PubMed
description Background and Aims: Coronary atherosclerosis is a chronic non-resolving inflammatory process wherein the interaction of innate immune cells and platelets plays a major role. Circulating neutrophils, in particular, adhere to the activated endothelium and migrate into the vascular wall, promoting monocyte recruitment and influencing plaque phenotype and stability at all stages of its evolution. We aimed to evaluate, by flow cytometry, if blood neutrophil number and phenotype—including their phenotypic relationships with platelets, monocytes and lymphocytes—have an association with lipid-rich necrotic core volume (LRNCV), a generic index of coronary plaque vulnerability, in a group of stable patients with chronic coronary syndrome (CCS). Methods: In 55 patients, (68.53 ± 1.07 years of age, mean ± SEM; 71% male), the total LRNCV in each subject was assessed by a quantitative analysis of all coronary plaques detected by computed tomography coronary angiography (CTCA) and was normalized to the total plaque volume. The expression of CD14, CD16, CD18, CD11b, HLA-DR, CD163, CCR2, CCR5, CX3CR1, CXCR4 and CD41a cell surface markers was quantified by flow cytometry. Adhesion molecules, cytokines and chemokines, as well as MMP9 plasma levels, were measured by ELISA. Results: On a per-patient basis, LRNCV values were positively associated, by a multiple regression analysis, with the neutrophil count (n°/µL) (p = 0.02), neutrophil/lymphocyte ratio (p = 0.007), neutrophil/platelet ratio (p = 0.01), neutrophil RFI CD11b expression (p = 0.02) and neutrophil–platelet adhesion index (p = 0.01). Significantly positive multiple regression associations of LRNCV values with phenotypic ratios between neutrophil RFI CD11b expression and several lymphocyte and monocyte surface markers were also observed. In the bivariate correlation analysis, a significantly positive association was found between RFI values of neutrophil–CD41a+ complexes and neutrophil RFI CD11b expression (p < 0.0001). Conclusions: These preliminary findings suggest that a sustained increase in circulating neutrophils, together with the up-regulation of the integrin/activation membrane neutrophil marker CD11b may contribute, through the progressive intra-plaque accumulation of necrotic/apoptotic cells exceeding the efferocytosis/anti-inflammatory capacity of infiltrating macrophages and lymphocytes, to the relative enlargement of the lipid-rich necrotic core volume of coronary plaques in stable CAD patients, thus increasing their individual risk of acute complication.
format Online
Article
Text
id pubmed-9958623
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-99586232023-02-26 Association of Circulating Neutrophils with Relative Volume of Lipid-Rich Necrotic Core of Coronary Plaques in Stable Patients: A Substudy of SMARTool European Project Sbrana, Silverio Cecchettini, Antonella Bastiani, Luca Mazzone, Annamaria Vozzi, Federico Caselli, Chiara Neglia, Danilo Clemente, Alberto Scholte, Arthur J. H. A. Parodi, Oberdan Pelosi, Gualtiero Rocchiccioli, Silvia Life (Basel) Article Background and Aims: Coronary atherosclerosis is a chronic non-resolving inflammatory process wherein the interaction of innate immune cells and platelets plays a major role. Circulating neutrophils, in particular, adhere to the activated endothelium and migrate into the vascular wall, promoting monocyte recruitment and influencing plaque phenotype and stability at all stages of its evolution. We aimed to evaluate, by flow cytometry, if blood neutrophil number and phenotype—including their phenotypic relationships with platelets, monocytes and lymphocytes—have an association with lipid-rich necrotic core volume (LRNCV), a generic index of coronary plaque vulnerability, in a group of stable patients with chronic coronary syndrome (CCS). Methods: In 55 patients, (68.53 ± 1.07 years of age, mean ± SEM; 71% male), the total LRNCV in each subject was assessed by a quantitative analysis of all coronary plaques detected by computed tomography coronary angiography (CTCA) and was normalized to the total plaque volume. The expression of CD14, CD16, CD18, CD11b, HLA-DR, CD163, CCR2, CCR5, CX3CR1, CXCR4 and CD41a cell surface markers was quantified by flow cytometry. Adhesion molecules, cytokines and chemokines, as well as MMP9 plasma levels, were measured by ELISA. Results: On a per-patient basis, LRNCV values were positively associated, by a multiple regression analysis, with the neutrophil count (n°/µL) (p = 0.02), neutrophil/lymphocyte ratio (p = 0.007), neutrophil/platelet ratio (p = 0.01), neutrophil RFI CD11b expression (p = 0.02) and neutrophil–platelet adhesion index (p = 0.01). Significantly positive multiple regression associations of LRNCV values with phenotypic ratios between neutrophil RFI CD11b expression and several lymphocyte and monocyte surface markers were also observed. In the bivariate correlation analysis, a significantly positive association was found between RFI values of neutrophil–CD41a+ complexes and neutrophil RFI CD11b expression (p < 0.0001). Conclusions: These preliminary findings suggest that a sustained increase in circulating neutrophils, together with the up-regulation of the integrin/activation membrane neutrophil marker CD11b may contribute, through the progressive intra-plaque accumulation of necrotic/apoptotic cells exceeding the efferocytosis/anti-inflammatory capacity of infiltrating macrophages and lymphocytes, to the relative enlargement of the lipid-rich necrotic core volume of coronary plaques in stable CAD patients, thus increasing their individual risk of acute complication. MDPI 2023-02-02 /pmc/articles/PMC9958623/ /pubmed/36836785 http://dx.doi.org/10.3390/life13020428 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sbrana, Silverio
Cecchettini, Antonella
Bastiani, Luca
Mazzone, Annamaria
Vozzi, Federico
Caselli, Chiara
Neglia, Danilo
Clemente, Alberto
Scholte, Arthur J. H. A.
Parodi, Oberdan
Pelosi, Gualtiero
Rocchiccioli, Silvia
Association of Circulating Neutrophils with Relative Volume of Lipid-Rich Necrotic Core of Coronary Plaques in Stable Patients: A Substudy of SMARTool European Project
title Association of Circulating Neutrophils with Relative Volume of Lipid-Rich Necrotic Core of Coronary Plaques in Stable Patients: A Substudy of SMARTool European Project
title_full Association of Circulating Neutrophils with Relative Volume of Lipid-Rich Necrotic Core of Coronary Plaques in Stable Patients: A Substudy of SMARTool European Project
title_fullStr Association of Circulating Neutrophils with Relative Volume of Lipid-Rich Necrotic Core of Coronary Plaques in Stable Patients: A Substudy of SMARTool European Project
title_full_unstemmed Association of Circulating Neutrophils with Relative Volume of Lipid-Rich Necrotic Core of Coronary Plaques in Stable Patients: A Substudy of SMARTool European Project
title_short Association of Circulating Neutrophils with Relative Volume of Lipid-Rich Necrotic Core of Coronary Plaques in Stable Patients: A Substudy of SMARTool European Project
title_sort association of circulating neutrophils with relative volume of lipid-rich necrotic core of coronary plaques in stable patients: a substudy of smartool european project
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958623/
https://www.ncbi.nlm.nih.gov/pubmed/36836785
http://dx.doi.org/10.3390/life13020428
work_keys_str_mv AT sbranasilverio associationofcirculatingneutrophilswithrelativevolumeoflipidrichnecroticcoreofcoronaryplaquesinstablepatientsasubstudyofsmartooleuropeanproject
AT cecchettiniantonella associationofcirculatingneutrophilswithrelativevolumeoflipidrichnecroticcoreofcoronaryplaquesinstablepatientsasubstudyofsmartooleuropeanproject
AT bastianiluca associationofcirculatingneutrophilswithrelativevolumeoflipidrichnecroticcoreofcoronaryplaquesinstablepatientsasubstudyofsmartooleuropeanproject
AT mazzoneannamaria associationofcirculatingneutrophilswithrelativevolumeoflipidrichnecroticcoreofcoronaryplaquesinstablepatientsasubstudyofsmartooleuropeanproject
AT vozzifederico associationofcirculatingneutrophilswithrelativevolumeoflipidrichnecroticcoreofcoronaryplaquesinstablepatientsasubstudyofsmartooleuropeanproject
AT casellichiara associationofcirculatingneutrophilswithrelativevolumeoflipidrichnecroticcoreofcoronaryplaquesinstablepatientsasubstudyofsmartooleuropeanproject
AT negliadanilo associationofcirculatingneutrophilswithrelativevolumeoflipidrichnecroticcoreofcoronaryplaquesinstablepatientsasubstudyofsmartooleuropeanproject
AT clementealberto associationofcirculatingneutrophilswithrelativevolumeoflipidrichnecroticcoreofcoronaryplaquesinstablepatientsasubstudyofsmartooleuropeanproject
AT scholtearthurjha associationofcirculatingneutrophilswithrelativevolumeoflipidrichnecroticcoreofcoronaryplaquesinstablepatientsasubstudyofsmartooleuropeanproject
AT parodioberdan associationofcirculatingneutrophilswithrelativevolumeoflipidrichnecroticcoreofcoronaryplaquesinstablepatientsasubstudyofsmartooleuropeanproject
AT pelosigualtiero associationofcirculatingneutrophilswithrelativevolumeoflipidrichnecroticcoreofcoronaryplaquesinstablepatientsasubstudyofsmartooleuropeanproject
AT rocchicciolisilvia associationofcirculatingneutrophilswithrelativevolumeoflipidrichnecroticcoreofcoronaryplaquesinstablepatientsasubstudyofsmartooleuropeanproject