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rMELEISH: A Novel Recombinant Multiepitope-Based Protein Applied to the Serodiagnosis of Both Canine and Human Visceral Leishmaniasis

Background: visceral leishmaniasis (VL) is a critical public health problem in over ninety countries. The control measures adopted in Brazil have been insufficient when it comes to preventing the spread of this overlooked disease. In this context, a precise diagnosis of VL in dogs and humans could h...

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Detalles Bibliográficos
Autores principales: Dias, Daniel Silva, Machado, Juliana Martins, Ribeiro, Patrícia Aparecida Fernandes, Machado, Amanda Sanchez, Ramos, Fernanda Fonseca, Nogueira, Lais Moreira, Gonçalves, Ana Alice Maia, Ramos, Luana de Sousa, Gandra, Isadora Braga, Coutinho, Flaviane Silva, dos Santos, Michelli, da Silva, Jonatas Oliveira, Chávez-Fumagalli, Miguel Angel, Teixeira-Neto, Rafael Gonçalves, Chaves, Ana Thereza, Campos-da-Paz, Mariana, Souza, Amanda A., Giunchetti, Rodolfo Cordeiro, Freitas, Sonia Maria, Lyon, Sandra, de Magalhães-Soares, Danielle Ferreira, Silveira, Julia Angelica Gonçalves, Silva, Eduardo Sergio, Coelho, Eduardo Antonio Ferraz, Galdino, Alexsandro Sobreira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958779/
https://www.ncbi.nlm.nih.gov/pubmed/36839574
http://dx.doi.org/10.3390/pathogens12020302
Descripción
Sumario:Background: visceral leishmaniasis (VL) is a critical public health problem in over ninety countries. The control measures adopted in Brazil have been insufficient when it comes to preventing the spread of this overlooked disease. In this context, a precise diagnosis of VL in dogs and humans could help to reduce the number of cases of this disease. Distinct studies for the diagnosis of VL have used single recombinant proteins in serological assays; however, the results have been variable, mainly in relation to the sensitivity of the antigens. In this context, the development of multiepitope-based proteins could be relevant to solving such problem. Methods: a chimeric protein (rMELEISH) was constructed based on amino acid sequences from kinesin 39 (k39), alpha-tubulin, and heat-shock proteins HSP70 and HSP 83.1, and tested in enzyme-linked immunosorbent (ELISA) for the detection of L. infantum infection using canine (n = 140) and human (n = 145) sera samples. Results: in the trials, rMELEISH was able to discriminate between VL cases and cross-reactive diseases and healthy samples, with sensitivity and specificity values of 100%, as compared to the use of a soluble Leishmania antigenic extract (SLA). Conclusions: the preliminary data suggest that rMELEISH has the potential to be tested in future studies against a larger serological panel and in field conditions for the diagnosis of canine and human VL.