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Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta
Thermophilic composting is a suitable treatment for the recycling of organic wastes for agriculture. However, using human excreta as feedstock for composting raises concerns about antibiotic resistances. We analyzed samples from the start and end of a thermophilic composting trial of human excreta,...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958827/ https://www.ncbi.nlm.nih.gov/pubmed/36838273 http://dx.doi.org/10.3390/microorganisms11020308 |
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author | Werner, Katharina A. Feyen, Lara Hübner, Tobias Brüggemann, Nicolas Prost, Katharina Grohmann, Elisabeth |
author_facet | Werner, Katharina A. Feyen, Lara Hübner, Tobias Brüggemann, Nicolas Prost, Katharina Grohmann, Elisabeth |
author_sort | Werner, Katharina A. |
collection | PubMed |
description | Thermophilic composting is a suitable treatment for the recycling of organic wastes for agriculture. However, using human excreta as feedstock for composting raises concerns about antibiotic resistances. We analyzed samples from the start and end of a thermophilic composting trial of human excreta, together with green cuttings and straw, with and without biochar. Beta-lactamase genes bla(CTX-M), bla(IMP), and bla(TEM) conferring resistance to broad-spectrum beta-lactam antibiotics, as well as horizontal gene transfer marker genes, intI1 and korB, were quantified using qPCR. We found low concentrations of the beta-lactamase genes in all samples, with non-significant mean decreases in bla(CTX-M) and bla(TEM) copy numbers and a mean increase in bla(IMP) copy numbers. The decrease in both intI1 and korB genes from start to end of composting indicated that thermophilic composting can decrease the horizontal spread of resistance genes. Thus, thermophilic composting can be a suitable treatment for the recycling of human excreta. |
format | Online Article Text |
id | pubmed-9958827 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99588272023-02-26 Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta Werner, Katharina A. Feyen, Lara Hübner, Tobias Brüggemann, Nicolas Prost, Katharina Grohmann, Elisabeth Microorganisms Communication Thermophilic composting is a suitable treatment for the recycling of organic wastes for agriculture. However, using human excreta as feedstock for composting raises concerns about antibiotic resistances. We analyzed samples from the start and end of a thermophilic composting trial of human excreta, together with green cuttings and straw, with and without biochar. Beta-lactamase genes bla(CTX-M), bla(IMP), and bla(TEM) conferring resistance to broad-spectrum beta-lactam antibiotics, as well as horizontal gene transfer marker genes, intI1 and korB, were quantified using qPCR. We found low concentrations of the beta-lactamase genes in all samples, with non-significant mean decreases in bla(CTX-M) and bla(TEM) copy numbers and a mean increase in bla(IMP) copy numbers. The decrease in both intI1 and korB genes from start to end of composting indicated that thermophilic composting can decrease the horizontal spread of resistance genes. Thus, thermophilic composting can be a suitable treatment for the recycling of human excreta. MDPI 2023-01-24 /pmc/articles/PMC9958827/ /pubmed/36838273 http://dx.doi.org/10.3390/microorganisms11020308 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Werner, Katharina A. Feyen, Lara Hübner, Tobias Brüggemann, Nicolas Prost, Katharina Grohmann, Elisabeth Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta |
title | Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta |
title_full | Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta |
title_fullStr | Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta |
title_full_unstemmed | Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta |
title_short | Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta |
title_sort | fate of horizontal-gene-transfer markers and beta-lactamase genes during thermophilic composting of human excreta |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958827/ https://www.ncbi.nlm.nih.gov/pubmed/36838273 http://dx.doi.org/10.3390/microorganisms11020308 |
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