Cargando…
The Effect of Super-Repressor IkB-Loaded Exosomes (Exo-srIκBs) in Chronic Post-Ischemia Pain (CPIP) Models
Complex regional pain syndrome (CRPS) is a condition associated with neuropathic pain that causes significant impairment of daily activities and functioning. Nuclear factor kappa B (NFκB) is thought to play an important role in the mechanism of CRPS. Recently, exosomes loaded with super-repressor in...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958867/ https://www.ncbi.nlm.nih.gov/pubmed/36839876 http://dx.doi.org/10.3390/pharmaceutics15020553 |
_version_ | 1784895131341553664 |
---|---|
author | Chae, Ji Seon Park, Hyunju Ahn, So-Hee Han, Eun-Chong Lee, Yoonjin Kim, Youn Jin Ahn, Eun-Jin Oh, Hye-Won Lee, Hyun Jung Choi, Chulhee Choi, Youn-Hee Kim, Won-joong |
author_facet | Chae, Ji Seon Park, Hyunju Ahn, So-Hee Han, Eun-Chong Lee, Yoonjin Kim, Youn Jin Ahn, Eun-Jin Oh, Hye-Won Lee, Hyun Jung Choi, Chulhee Choi, Youn-Hee Kim, Won-joong |
author_sort | Chae, Ji Seon |
collection | PubMed |
description | Complex regional pain syndrome (CRPS) is a condition associated with neuropathic pain that causes significant impairment of daily activities and functioning. Nuclear factor kappa B (NFκB) is thought to play an important role in the mechanism of CRPS. Recently, exosomes loaded with super-repressor inhibitory kappa B (Exo-srIκB, IκB; inhibitor of NFκB) have been shown to have potential anti-inflammatory effects in various inflammatory disease models. We investigated the therapeutic effect of Exo-srIκB on a rodent model with chronic post-ischemia pain (CPIP), a representative animal model of Type I CRPS. After intraperitoneal injection of a vehicle, Exo-srIκB, and pregabalin, the paw withdrawal threshold (PWT) was evaluated up to 48 h. Administration of Exo-srIκB increased PWT compared to the vehicle and pregabalin, and the relative densities of p-IκB and IκB showed significant changes compared to the vehicle 24 h after Exo-srIκB injection. The levels of several cytokines and chemokines were reduced by the administration of Exo-srIκB in mice with CPIP. In conclusion, our results showed more specifically the role of NFκB in the pathogenesis of CRPS and provided a theoretical background for novel treatment options for CRPS. |
format | Online Article Text |
id | pubmed-9958867 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99588672023-02-26 The Effect of Super-Repressor IkB-Loaded Exosomes (Exo-srIκBs) in Chronic Post-Ischemia Pain (CPIP) Models Chae, Ji Seon Park, Hyunju Ahn, So-Hee Han, Eun-Chong Lee, Yoonjin Kim, Youn Jin Ahn, Eun-Jin Oh, Hye-Won Lee, Hyun Jung Choi, Chulhee Choi, Youn-Hee Kim, Won-joong Pharmaceutics Article Complex regional pain syndrome (CRPS) is a condition associated with neuropathic pain that causes significant impairment of daily activities and functioning. Nuclear factor kappa B (NFκB) is thought to play an important role in the mechanism of CRPS. Recently, exosomes loaded with super-repressor inhibitory kappa B (Exo-srIκB, IκB; inhibitor of NFκB) have been shown to have potential anti-inflammatory effects in various inflammatory disease models. We investigated the therapeutic effect of Exo-srIκB on a rodent model with chronic post-ischemia pain (CPIP), a representative animal model of Type I CRPS. After intraperitoneal injection of a vehicle, Exo-srIκB, and pregabalin, the paw withdrawal threshold (PWT) was evaluated up to 48 h. Administration of Exo-srIκB increased PWT compared to the vehicle and pregabalin, and the relative densities of p-IκB and IκB showed significant changes compared to the vehicle 24 h after Exo-srIκB injection. The levels of several cytokines and chemokines were reduced by the administration of Exo-srIκB in mice with CPIP. In conclusion, our results showed more specifically the role of NFκB in the pathogenesis of CRPS and provided a theoretical background for novel treatment options for CRPS. MDPI 2023-02-07 /pmc/articles/PMC9958867/ /pubmed/36839876 http://dx.doi.org/10.3390/pharmaceutics15020553 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chae, Ji Seon Park, Hyunju Ahn, So-Hee Han, Eun-Chong Lee, Yoonjin Kim, Youn Jin Ahn, Eun-Jin Oh, Hye-Won Lee, Hyun Jung Choi, Chulhee Choi, Youn-Hee Kim, Won-joong The Effect of Super-Repressor IkB-Loaded Exosomes (Exo-srIκBs) in Chronic Post-Ischemia Pain (CPIP) Models |
title | The Effect of Super-Repressor IkB-Loaded Exosomes (Exo-srIκBs) in Chronic Post-Ischemia Pain (CPIP) Models |
title_full | The Effect of Super-Repressor IkB-Loaded Exosomes (Exo-srIκBs) in Chronic Post-Ischemia Pain (CPIP) Models |
title_fullStr | The Effect of Super-Repressor IkB-Loaded Exosomes (Exo-srIκBs) in Chronic Post-Ischemia Pain (CPIP) Models |
title_full_unstemmed | The Effect of Super-Repressor IkB-Loaded Exosomes (Exo-srIκBs) in Chronic Post-Ischemia Pain (CPIP) Models |
title_short | The Effect of Super-Repressor IkB-Loaded Exosomes (Exo-srIκBs) in Chronic Post-Ischemia Pain (CPIP) Models |
title_sort | effect of super-repressor ikb-loaded exosomes (exo-sriκbs) in chronic post-ischemia pain (cpip) models |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958867/ https://www.ncbi.nlm.nih.gov/pubmed/36839876 http://dx.doi.org/10.3390/pharmaceutics15020553 |
work_keys_str_mv | AT chaejiseon theeffectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels AT parkhyunju theeffectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels AT ahnsohee theeffectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels AT haneunchong theeffectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels AT leeyoonjin theeffectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels AT kimyounjin theeffectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels AT ahneunjin theeffectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels AT ohhyewon theeffectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels AT leehyunjung theeffectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels AT choichulhee theeffectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels AT choiyounhee theeffectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels AT kimwonjoong theeffectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels AT chaejiseon effectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels AT parkhyunju effectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels AT ahnsohee effectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels AT haneunchong effectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels AT leeyoonjin effectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels AT kimyounjin effectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels AT ahneunjin effectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels AT ohhyewon effectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels AT leehyunjung effectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels AT choichulhee effectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels AT choiyounhee effectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels AT kimwonjoong effectofsuperrepressorikbloadedexosomesexosrikbsinchronicpostischemiapaincpipmodels |