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Dual Effect of Chemo-PDT with Tumor Targeting Nanoparticles Containing iRGD Peptide
Nanotechnology, including self-aggregated nanoparticles, has shown high effectiveness in the treatment of solid tumors. To overcome the limitations of conventional cancer therapies and promote therapeutic efficacy, a combination of PDT and chemotherapy can be considered an effective strategy for can...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959063/ https://www.ncbi.nlm.nih.gov/pubmed/36839936 http://dx.doi.org/10.3390/pharmaceutics15020614 |
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author | Kim, Gye Lim Park, Byeongmin Jang, Eun Hyang Gu, Jaeun Seo, Seo Ra Cheung, Hyein Lee, Hyo Jung Lee, Sangmin Kim, Jong-Ho |
author_facet | Kim, Gye Lim Park, Byeongmin Jang, Eun Hyang Gu, Jaeun Seo, Seo Ra Cheung, Hyein Lee, Hyo Jung Lee, Sangmin Kim, Jong-Ho |
author_sort | Kim, Gye Lim |
collection | PubMed |
description | Nanotechnology, including self-aggregated nanoparticles, has shown high effectiveness in the treatment of solid tumors. To overcome the limitations of conventional cancer therapies and promote therapeutic efficacy, a combination of PDT and chemotherapy can be considered an effective strategy for cancer treatment. This study presents the development of tumor-targeting polysialic acid (PSA) nanoparticles for chemo-PDT to increase the cellular uptake and cytotoxic effect in cancer cells. Chlorin e6 (Ce6), a photosensitizer, and the iRGD peptide (sequence; cCRGDKGPDC) were conjugated to the amine of N-deacetylated PSA. They generate reactive oxygen species (ROS), especially singlet oxygen ((1)O(2)), and target integrin αvβ3 on the cancer cell surface. To offer a chemotherapeutic effect, doxorubicin (Dox) was assembled into the core of hydrophobically modified PSA by connecting it with Ce6; this was followed by its sustained release from the nanoparticles. These nanoparticles are able to generate ROS under 633 nm visible-light irradiation, resulting in the strong cytotoxicity of Dox with anticancer effects in HCT116 cells. PSA nanoparticles with the dual effect of chemo-PDT improve conventional PDT, which has a poor ability to deliver photosensitizers to cancer cells. Using their combination with Dox chemotherapy, rapid removal of cancer cells can be expected. |
format | Online Article Text |
id | pubmed-9959063 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99590632023-02-26 Dual Effect of Chemo-PDT with Tumor Targeting Nanoparticles Containing iRGD Peptide Kim, Gye Lim Park, Byeongmin Jang, Eun Hyang Gu, Jaeun Seo, Seo Ra Cheung, Hyein Lee, Hyo Jung Lee, Sangmin Kim, Jong-Ho Pharmaceutics Article Nanotechnology, including self-aggregated nanoparticles, has shown high effectiveness in the treatment of solid tumors. To overcome the limitations of conventional cancer therapies and promote therapeutic efficacy, a combination of PDT and chemotherapy can be considered an effective strategy for cancer treatment. This study presents the development of tumor-targeting polysialic acid (PSA) nanoparticles for chemo-PDT to increase the cellular uptake and cytotoxic effect in cancer cells. Chlorin e6 (Ce6), a photosensitizer, and the iRGD peptide (sequence; cCRGDKGPDC) were conjugated to the amine of N-deacetylated PSA. They generate reactive oxygen species (ROS), especially singlet oxygen ((1)O(2)), and target integrin αvβ3 on the cancer cell surface. To offer a chemotherapeutic effect, doxorubicin (Dox) was assembled into the core of hydrophobically modified PSA by connecting it with Ce6; this was followed by its sustained release from the nanoparticles. These nanoparticles are able to generate ROS under 633 nm visible-light irradiation, resulting in the strong cytotoxicity of Dox with anticancer effects in HCT116 cells. PSA nanoparticles with the dual effect of chemo-PDT improve conventional PDT, which has a poor ability to deliver photosensitizers to cancer cells. Using their combination with Dox chemotherapy, rapid removal of cancer cells can be expected. MDPI 2023-02-11 /pmc/articles/PMC9959063/ /pubmed/36839936 http://dx.doi.org/10.3390/pharmaceutics15020614 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Gye Lim Park, Byeongmin Jang, Eun Hyang Gu, Jaeun Seo, Seo Ra Cheung, Hyein Lee, Hyo Jung Lee, Sangmin Kim, Jong-Ho Dual Effect of Chemo-PDT with Tumor Targeting Nanoparticles Containing iRGD Peptide |
title | Dual Effect of Chemo-PDT with Tumor Targeting Nanoparticles Containing iRGD Peptide |
title_full | Dual Effect of Chemo-PDT with Tumor Targeting Nanoparticles Containing iRGD Peptide |
title_fullStr | Dual Effect of Chemo-PDT with Tumor Targeting Nanoparticles Containing iRGD Peptide |
title_full_unstemmed | Dual Effect of Chemo-PDT with Tumor Targeting Nanoparticles Containing iRGD Peptide |
title_short | Dual Effect of Chemo-PDT with Tumor Targeting Nanoparticles Containing iRGD Peptide |
title_sort | dual effect of chemo-pdt with tumor targeting nanoparticles containing irgd peptide |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959063/ https://www.ncbi.nlm.nih.gov/pubmed/36839936 http://dx.doi.org/10.3390/pharmaceutics15020614 |
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