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Dual Effect of Chemo-PDT with Tumor Targeting Nanoparticles Containing iRGD Peptide

Nanotechnology, including self-aggregated nanoparticles, has shown high effectiveness in the treatment of solid tumors. To overcome the limitations of conventional cancer therapies and promote therapeutic efficacy, a combination of PDT and chemotherapy can be considered an effective strategy for can...

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Autores principales: Kim, Gye Lim, Park, Byeongmin, Jang, Eun Hyang, Gu, Jaeun, Seo, Seo Ra, Cheung, Hyein, Lee, Hyo Jung, Lee, Sangmin, Kim, Jong-Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959063/
https://www.ncbi.nlm.nih.gov/pubmed/36839936
http://dx.doi.org/10.3390/pharmaceutics15020614
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author Kim, Gye Lim
Park, Byeongmin
Jang, Eun Hyang
Gu, Jaeun
Seo, Seo Ra
Cheung, Hyein
Lee, Hyo Jung
Lee, Sangmin
Kim, Jong-Ho
author_facet Kim, Gye Lim
Park, Byeongmin
Jang, Eun Hyang
Gu, Jaeun
Seo, Seo Ra
Cheung, Hyein
Lee, Hyo Jung
Lee, Sangmin
Kim, Jong-Ho
author_sort Kim, Gye Lim
collection PubMed
description Nanotechnology, including self-aggregated nanoparticles, has shown high effectiveness in the treatment of solid tumors. To overcome the limitations of conventional cancer therapies and promote therapeutic efficacy, a combination of PDT and chemotherapy can be considered an effective strategy for cancer treatment. This study presents the development of tumor-targeting polysialic acid (PSA) nanoparticles for chemo-PDT to increase the cellular uptake and cytotoxic effect in cancer cells. Chlorin e6 (Ce6), a photosensitizer, and the iRGD peptide (sequence; cCRGDKGPDC) were conjugated to the amine of N-deacetylated PSA. They generate reactive oxygen species (ROS), especially singlet oxygen ((1)O(2)), and target integrin αvβ3 on the cancer cell surface. To offer a chemotherapeutic effect, doxorubicin (Dox) was assembled into the core of hydrophobically modified PSA by connecting it with Ce6; this was followed by its sustained release from the nanoparticles. These nanoparticles are able to generate ROS under 633 nm visible-light irradiation, resulting in the strong cytotoxicity of Dox with anticancer effects in HCT116 cells. PSA nanoparticles with the dual effect of chemo-PDT improve conventional PDT, which has a poor ability to deliver photosensitizers to cancer cells. Using their combination with Dox chemotherapy, rapid removal of cancer cells can be expected.
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spelling pubmed-99590632023-02-26 Dual Effect of Chemo-PDT with Tumor Targeting Nanoparticles Containing iRGD Peptide Kim, Gye Lim Park, Byeongmin Jang, Eun Hyang Gu, Jaeun Seo, Seo Ra Cheung, Hyein Lee, Hyo Jung Lee, Sangmin Kim, Jong-Ho Pharmaceutics Article Nanotechnology, including self-aggregated nanoparticles, has shown high effectiveness in the treatment of solid tumors. To overcome the limitations of conventional cancer therapies and promote therapeutic efficacy, a combination of PDT and chemotherapy can be considered an effective strategy for cancer treatment. This study presents the development of tumor-targeting polysialic acid (PSA) nanoparticles for chemo-PDT to increase the cellular uptake and cytotoxic effect in cancer cells. Chlorin e6 (Ce6), a photosensitizer, and the iRGD peptide (sequence; cCRGDKGPDC) were conjugated to the amine of N-deacetylated PSA. They generate reactive oxygen species (ROS), especially singlet oxygen ((1)O(2)), and target integrin αvβ3 on the cancer cell surface. To offer a chemotherapeutic effect, doxorubicin (Dox) was assembled into the core of hydrophobically modified PSA by connecting it with Ce6; this was followed by its sustained release from the nanoparticles. These nanoparticles are able to generate ROS under 633 nm visible-light irradiation, resulting in the strong cytotoxicity of Dox with anticancer effects in HCT116 cells. PSA nanoparticles with the dual effect of chemo-PDT improve conventional PDT, which has a poor ability to deliver photosensitizers to cancer cells. Using their combination with Dox chemotherapy, rapid removal of cancer cells can be expected. MDPI 2023-02-11 /pmc/articles/PMC9959063/ /pubmed/36839936 http://dx.doi.org/10.3390/pharmaceutics15020614 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Gye Lim
Park, Byeongmin
Jang, Eun Hyang
Gu, Jaeun
Seo, Seo Ra
Cheung, Hyein
Lee, Hyo Jung
Lee, Sangmin
Kim, Jong-Ho
Dual Effect of Chemo-PDT with Tumor Targeting Nanoparticles Containing iRGD Peptide
title Dual Effect of Chemo-PDT with Tumor Targeting Nanoparticles Containing iRGD Peptide
title_full Dual Effect of Chemo-PDT with Tumor Targeting Nanoparticles Containing iRGD Peptide
title_fullStr Dual Effect of Chemo-PDT with Tumor Targeting Nanoparticles Containing iRGD Peptide
title_full_unstemmed Dual Effect of Chemo-PDT with Tumor Targeting Nanoparticles Containing iRGD Peptide
title_short Dual Effect of Chemo-PDT with Tumor Targeting Nanoparticles Containing iRGD Peptide
title_sort dual effect of chemo-pdt with tumor targeting nanoparticles containing irgd peptide
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959063/
https://www.ncbi.nlm.nih.gov/pubmed/36839936
http://dx.doi.org/10.3390/pharmaceutics15020614
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