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Helicobacter pylori Virulence Factors and Clarithromycin Resistance-Associated Mutations in Mexican Patients

Persistent infection with Helicobacter pylori (H. pylori) is an important factor in gastric diseases. The vacA and cagA virulence factors of H. pylori contribute to the development of these diseases. Triple therapy containing clarithromycin has been used to eradicate this infection. Unfortunately, r...

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Autores principales: Alarcón-Millán, Judit, Bonilla-Delgado, José, Fernández-Tilapa, Gloria, Nieto-Velázquez, Nayeli Goreti, Sierra-Martínez, Mónica, Alvarado-Castro, Víctor Manuel, Cortés-Malagón, Enoc Mariano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959081/
https://www.ncbi.nlm.nih.gov/pubmed/36839506
http://dx.doi.org/10.3390/pathogens12020234
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author Alarcón-Millán, Judit
Bonilla-Delgado, José
Fernández-Tilapa, Gloria
Nieto-Velázquez, Nayeli Goreti
Sierra-Martínez, Mónica
Alvarado-Castro, Víctor Manuel
Cortés-Malagón, Enoc Mariano
author_facet Alarcón-Millán, Judit
Bonilla-Delgado, José
Fernández-Tilapa, Gloria
Nieto-Velázquez, Nayeli Goreti
Sierra-Martínez, Mónica
Alvarado-Castro, Víctor Manuel
Cortés-Malagón, Enoc Mariano
author_sort Alarcón-Millán, Judit
collection PubMed
description Persistent infection with Helicobacter pylori (H. pylori) is an important factor in gastric diseases. The vacA and cagA virulence factors of H. pylori contribute to the development of these diseases. Triple therapy containing clarithromycin has been used to eradicate this infection. Unfortunately, resistance to this antibiotic is the primary cause of treatment failure. This study aimed to determine the prevalence of clarithromycin resistance-associated mutations and to assess the relationship between virulence factors and Mexican patients infected with H. pylori. The cagA and vacA genotypes were determined by multiplex PCR. Furthermore, a qPCR was used to identify mutations of the 23S rRNA gene. This study reported a prevalence of 84.3% of H. pylori among patients with gastric diseases, and the vacA s1m1/cagA+ genotype was the most frequent (44.8%) in antrum and corpus. Analysis of the 23S rRNA gene revealed a 19.8% prevalence of clarithromycin resistance-associated mutations. The most prevalent mutations were A2143G (56%) and A2142C (25%). A significant association (p < 0.05) between the A2142G and the vacA s1m1/cagA+ genotype was detected. In conclusion, we report a high prevalence (>15%) of clarithromycin resistance-associated mutations, and we found an association between the genotypes of virulence factors and a mutation in the 23S rRNA gene.
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spelling pubmed-99590812023-02-26 Helicobacter pylori Virulence Factors and Clarithromycin Resistance-Associated Mutations in Mexican Patients Alarcón-Millán, Judit Bonilla-Delgado, José Fernández-Tilapa, Gloria Nieto-Velázquez, Nayeli Goreti Sierra-Martínez, Mónica Alvarado-Castro, Víctor Manuel Cortés-Malagón, Enoc Mariano Pathogens Article Persistent infection with Helicobacter pylori (H. pylori) is an important factor in gastric diseases. The vacA and cagA virulence factors of H. pylori contribute to the development of these diseases. Triple therapy containing clarithromycin has been used to eradicate this infection. Unfortunately, resistance to this antibiotic is the primary cause of treatment failure. This study aimed to determine the prevalence of clarithromycin resistance-associated mutations and to assess the relationship between virulence factors and Mexican patients infected with H. pylori. The cagA and vacA genotypes were determined by multiplex PCR. Furthermore, a qPCR was used to identify mutations of the 23S rRNA gene. This study reported a prevalence of 84.3% of H. pylori among patients with gastric diseases, and the vacA s1m1/cagA+ genotype was the most frequent (44.8%) in antrum and corpus. Analysis of the 23S rRNA gene revealed a 19.8% prevalence of clarithromycin resistance-associated mutations. The most prevalent mutations were A2143G (56%) and A2142C (25%). A significant association (p < 0.05) between the A2142G and the vacA s1m1/cagA+ genotype was detected. In conclusion, we report a high prevalence (>15%) of clarithromycin resistance-associated mutations, and we found an association between the genotypes of virulence factors and a mutation in the 23S rRNA gene. MDPI 2023-02-02 /pmc/articles/PMC9959081/ /pubmed/36839506 http://dx.doi.org/10.3390/pathogens12020234 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alarcón-Millán, Judit
Bonilla-Delgado, José
Fernández-Tilapa, Gloria
Nieto-Velázquez, Nayeli Goreti
Sierra-Martínez, Mónica
Alvarado-Castro, Víctor Manuel
Cortés-Malagón, Enoc Mariano
Helicobacter pylori Virulence Factors and Clarithromycin Resistance-Associated Mutations in Mexican Patients
title Helicobacter pylori Virulence Factors and Clarithromycin Resistance-Associated Mutations in Mexican Patients
title_full Helicobacter pylori Virulence Factors and Clarithromycin Resistance-Associated Mutations in Mexican Patients
title_fullStr Helicobacter pylori Virulence Factors and Clarithromycin Resistance-Associated Mutations in Mexican Patients
title_full_unstemmed Helicobacter pylori Virulence Factors and Clarithromycin Resistance-Associated Mutations in Mexican Patients
title_short Helicobacter pylori Virulence Factors and Clarithromycin Resistance-Associated Mutations in Mexican Patients
title_sort helicobacter pylori virulence factors and clarithromycin resistance-associated mutations in mexican patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959081/
https://www.ncbi.nlm.nih.gov/pubmed/36839506
http://dx.doi.org/10.3390/pathogens12020234
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