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3D Matrices for Enhanced Encapsulation and Controlled Release of Anti-Inflammatory Bioactive Compounds in Wound Healing

Current trends in the development of wound dressings are oriented towards the use of biopolymer-based materials, due to their unique properties such as non-toxicity, hydrophilicity, biocompatibility and biodegradability, properties that have advantageous therapeutic characteristics. In this regard,...

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Autores principales: Nicu, Raluca, Ciolacu, Diana Elena, Petrovici, Anca-Roxana, Rusu, Daniela, Avadanei, Mihaela, Mihaila, Andreea Cristina, Butoi, Elena, Ciolacu, Florin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959390/
https://www.ncbi.nlm.nih.gov/pubmed/36835619
http://dx.doi.org/10.3390/ijms24044213
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author Nicu, Raluca
Ciolacu, Diana Elena
Petrovici, Anca-Roxana
Rusu, Daniela
Avadanei, Mihaela
Mihaila, Andreea Cristina
Butoi, Elena
Ciolacu, Florin
author_facet Nicu, Raluca
Ciolacu, Diana Elena
Petrovici, Anca-Roxana
Rusu, Daniela
Avadanei, Mihaela
Mihaila, Andreea Cristina
Butoi, Elena
Ciolacu, Florin
author_sort Nicu, Raluca
collection PubMed
description Current trends in the development of wound dressings are oriented towards the use of biopolymer-based materials, due to their unique properties such as non-toxicity, hydrophilicity, biocompatibility and biodegradability, properties that have advantageous therapeutic characteristics. In this regard, the present study aims to develop hydrogels based on cellulose and dextran (CD) and to reveal their anti-inflammatory performance. This purpose is achieved by incorporating plant bioactive polyphenols (PFs) in CD hydrogels. The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels’ cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels. The results show that the presence of dextran has a positive impact on the hydrogel’s structure by decreasing the pore size at the same time as increasing the uniformity and interconnectivity of the pores. In addition, there is an increased degree of swelling and of the encapsulation capacity of PFs, with the increase of the dextran content in hydrogels. The kinetics of PFs released by hydrogels was studied according to the Korsmeyer–Peppas model, and it was observed that the transport mechanisms depend on hydrogels’ composition and morphology. Furthermore, CD hydrogels have been shown to promote cell proliferation without cytotoxicity, by successfully culturing fibroblasts and endothelial cells on CD hydrogels (over 80% viability). The anti-inflammatory tests performed in the presence of lipopolysaccharides demonstrate the anti-inflammatory properties of the PFs-loaded hydrogels. All these results provide conclusive evidence on the acceleration of wound healing by inhibiting the inflammation process and support the use of these hydrogels encapsulated with PFs in wound healing applications.
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spelling pubmed-99593902023-02-26 3D Matrices for Enhanced Encapsulation and Controlled Release of Anti-Inflammatory Bioactive Compounds in Wound Healing Nicu, Raluca Ciolacu, Diana Elena Petrovici, Anca-Roxana Rusu, Daniela Avadanei, Mihaela Mihaila, Andreea Cristina Butoi, Elena Ciolacu, Florin Int J Mol Sci Article Current trends in the development of wound dressings are oriented towards the use of biopolymer-based materials, due to their unique properties such as non-toxicity, hydrophilicity, biocompatibility and biodegradability, properties that have advantageous therapeutic characteristics. In this regard, the present study aims to develop hydrogels based on cellulose and dextran (CD) and to reveal their anti-inflammatory performance. This purpose is achieved by incorporating plant bioactive polyphenols (PFs) in CD hydrogels. The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels’ cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels. The results show that the presence of dextran has a positive impact on the hydrogel’s structure by decreasing the pore size at the same time as increasing the uniformity and interconnectivity of the pores. In addition, there is an increased degree of swelling and of the encapsulation capacity of PFs, with the increase of the dextran content in hydrogels. The kinetics of PFs released by hydrogels was studied according to the Korsmeyer–Peppas model, and it was observed that the transport mechanisms depend on hydrogels’ composition and morphology. Furthermore, CD hydrogels have been shown to promote cell proliferation without cytotoxicity, by successfully culturing fibroblasts and endothelial cells on CD hydrogels (over 80% viability). The anti-inflammatory tests performed in the presence of lipopolysaccharides demonstrate the anti-inflammatory properties of the PFs-loaded hydrogels. All these results provide conclusive evidence on the acceleration of wound healing by inhibiting the inflammation process and support the use of these hydrogels encapsulated with PFs in wound healing applications. MDPI 2023-02-20 /pmc/articles/PMC9959390/ /pubmed/36835619 http://dx.doi.org/10.3390/ijms24044213 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nicu, Raluca
Ciolacu, Diana Elena
Petrovici, Anca-Roxana
Rusu, Daniela
Avadanei, Mihaela
Mihaila, Andreea Cristina
Butoi, Elena
Ciolacu, Florin
3D Matrices for Enhanced Encapsulation and Controlled Release of Anti-Inflammatory Bioactive Compounds in Wound Healing
title 3D Matrices for Enhanced Encapsulation and Controlled Release of Anti-Inflammatory Bioactive Compounds in Wound Healing
title_full 3D Matrices for Enhanced Encapsulation and Controlled Release of Anti-Inflammatory Bioactive Compounds in Wound Healing
title_fullStr 3D Matrices for Enhanced Encapsulation and Controlled Release of Anti-Inflammatory Bioactive Compounds in Wound Healing
title_full_unstemmed 3D Matrices for Enhanced Encapsulation and Controlled Release of Anti-Inflammatory Bioactive Compounds in Wound Healing
title_short 3D Matrices for Enhanced Encapsulation and Controlled Release of Anti-Inflammatory Bioactive Compounds in Wound Healing
title_sort 3d matrices for enhanced encapsulation and controlled release of anti-inflammatory bioactive compounds in wound healing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959390/
https://www.ncbi.nlm.nih.gov/pubmed/36835619
http://dx.doi.org/10.3390/ijms24044213
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