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In Vitro Effectiveness of Soft Contact Lens Solutions Available on the Dutch Market against Acanthamoeba Species

Acanthamoeba keratitis is almost universally associated with contact lens (CL) use. Until today, however, CL solution manufacturing protocols lack testing of anti-amoebic activity. This study investigates the effectiveness of CL solutions available on the Dutch market against trophozoites and cysts...

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Autores principales: Randag, Anna C., de Kroon, Lieke, Otten, Henny, Arias Claro-Handgraaf, Cindy, Schimmer, Barbara, Kortbeek, Titia, van Rooij, Jeroen, Stelma, Foekje F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959425/
https://www.ncbi.nlm.nih.gov/pubmed/36839486
http://dx.doi.org/10.3390/pathogens12020214
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author Randag, Anna C.
de Kroon, Lieke
Otten, Henny
Arias Claro-Handgraaf, Cindy
Schimmer, Barbara
Kortbeek, Titia
van Rooij, Jeroen
Stelma, Foekje F.
author_facet Randag, Anna C.
de Kroon, Lieke
Otten, Henny
Arias Claro-Handgraaf, Cindy
Schimmer, Barbara
Kortbeek, Titia
van Rooij, Jeroen
Stelma, Foekje F.
author_sort Randag, Anna C.
collection PubMed
description Acanthamoeba keratitis is almost universally associated with contact lens (CL) use. Until today, however, CL solution manufacturing protocols lack testing of anti-amoebic activity. This study investigates the effectiveness of CL solutions available on the Dutch market against trophozoites and cysts of Acanthamoeba castellanii and Acanthamoeba polyphaga. Sixteen CL solutions were tested: 13 multiple purpose solutions (MPS), 2 hydrogen peroxidase solutions (HPS) and 1 povidone-iodine-based solution (PIS). The Spearman–Karber (SK) log reduction method and an XTT colorimetric assay were used to evaluate the effectiveness at the manufacturer’s minimum recommended disinfection time (MMRDT) and after eight hours. At the MMRDT, one MPS showed an SK mean log reduction (MLR) of >3.0 against A. castellanii trophozoites. Two additional MPS and both HPS reached this threshold after eight hours. The SK MLR values for A. polyphaga trophozoites were between 1 and 3 at all time points. Using the XTT colorimetric assay, only HPS 1 showed >99.9% reduction (equivalent to 3 log reduction) in metabolic activity of A. castellanii trophozoites after eight hours. For A. polyphaga, both HPS and PIS showed a metabolic reduction of >99.9% after eight hours. Cysts were resistant against all solutions. We conclude that following the manufacturer’s guidelines, few solutions provide sufficient effectiveness against Acanthamoeba trophozoites and none against cysts. The results underline the importance of adequate hygiene when handling CLs.
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spelling pubmed-99594252023-02-26 In Vitro Effectiveness of Soft Contact Lens Solutions Available on the Dutch Market against Acanthamoeba Species Randag, Anna C. de Kroon, Lieke Otten, Henny Arias Claro-Handgraaf, Cindy Schimmer, Barbara Kortbeek, Titia van Rooij, Jeroen Stelma, Foekje F. Pathogens Brief Report Acanthamoeba keratitis is almost universally associated with contact lens (CL) use. Until today, however, CL solution manufacturing protocols lack testing of anti-amoebic activity. This study investigates the effectiveness of CL solutions available on the Dutch market against trophozoites and cysts of Acanthamoeba castellanii and Acanthamoeba polyphaga. Sixteen CL solutions were tested: 13 multiple purpose solutions (MPS), 2 hydrogen peroxidase solutions (HPS) and 1 povidone-iodine-based solution (PIS). The Spearman–Karber (SK) log reduction method and an XTT colorimetric assay were used to evaluate the effectiveness at the manufacturer’s minimum recommended disinfection time (MMRDT) and after eight hours. At the MMRDT, one MPS showed an SK mean log reduction (MLR) of >3.0 against A. castellanii trophozoites. Two additional MPS and both HPS reached this threshold after eight hours. The SK MLR values for A. polyphaga trophozoites were between 1 and 3 at all time points. Using the XTT colorimetric assay, only HPS 1 showed >99.9% reduction (equivalent to 3 log reduction) in metabolic activity of A. castellanii trophozoites after eight hours. For A. polyphaga, both HPS and PIS showed a metabolic reduction of >99.9% after eight hours. Cysts were resistant against all solutions. We conclude that following the manufacturer’s guidelines, few solutions provide sufficient effectiveness against Acanthamoeba trophozoites and none against cysts. The results underline the importance of adequate hygiene when handling CLs. MDPI 2023-01-29 /pmc/articles/PMC9959425/ /pubmed/36839486 http://dx.doi.org/10.3390/pathogens12020214 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Randag, Anna C.
de Kroon, Lieke
Otten, Henny
Arias Claro-Handgraaf, Cindy
Schimmer, Barbara
Kortbeek, Titia
van Rooij, Jeroen
Stelma, Foekje F.
In Vitro Effectiveness of Soft Contact Lens Solutions Available on the Dutch Market against Acanthamoeba Species
title In Vitro Effectiveness of Soft Contact Lens Solutions Available on the Dutch Market against Acanthamoeba Species
title_full In Vitro Effectiveness of Soft Contact Lens Solutions Available on the Dutch Market against Acanthamoeba Species
title_fullStr In Vitro Effectiveness of Soft Contact Lens Solutions Available on the Dutch Market against Acanthamoeba Species
title_full_unstemmed In Vitro Effectiveness of Soft Contact Lens Solutions Available on the Dutch Market against Acanthamoeba Species
title_short In Vitro Effectiveness of Soft Contact Lens Solutions Available on the Dutch Market against Acanthamoeba Species
title_sort in vitro effectiveness of soft contact lens solutions available on the dutch market against acanthamoeba species
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959425/
https://www.ncbi.nlm.nih.gov/pubmed/36839486
http://dx.doi.org/10.3390/pathogens12020214
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