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Promising Antifungal Molecules against Mucormycosis Agents Identified from Pandemic Response Box(®): In Vitro and In Silico Analyses

Mucormycosis is considered concerning invasive fungal infections due to its high mortality rates, difficult diagnosis and limited treatment approaches. Mucorales species are highly resistant to many antifungal agents and the search for alternatives is an urgent need. In the present study, a library...

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Autores principales: Xisto, Mariana Ingrid Dutra da Silva, Rollin-Pinheiro, Rodrigo, de Castro-Almeida, Yuri, dos Santos-Freitas, Giulia Maria Pires, Rochetti, Victor Pereira, Borba-Santos, Luana Pereira, da Silva Fontes, Yasmin, Ferreira-Pereira, Antonio, Rozental, Sonia, Barreto-Bergter, Eliana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959553/
https://www.ncbi.nlm.nih.gov/pubmed/36836302
http://dx.doi.org/10.3390/jof9020187
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author Xisto, Mariana Ingrid Dutra da Silva
Rollin-Pinheiro, Rodrigo
de Castro-Almeida, Yuri
dos Santos-Freitas, Giulia Maria Pires
Rochetti, Victor Pereira
Borba-Santos, Luana Pereira
da Silva Fontes, Yasmin
Ferreira-Pereira, Antonio
Rozental, Sonia
Barreto-Bergter, Eliana
author_facet Xisto, Mariana Ingrid Dutra da Silva
Rollin-Pinheiro, Rodrigo
de Castro-Almeida, Yuri
dos Santos-Freitas, Giulia Maria Pires
Rochetti, Victor Pereira
Borba-Santos, Luana Pereira
da Silva Fontes, Yasmin
Ferreira-Pereira, Antonio
Rozental, Sonia
Barreto-Bergter, Eliana
author_sort Xisto, Mariana Ingrid Dutra da Silva
collection PubMed
description Mucormycosis is considered concerning invasive fungal infections due to its high mortality rates, difficult diagnosis and limited treatment approaches. Mucorales species are highly resistant to many antifungal agents and the search for alternatives is an urgent need. In the present study, a library with 400 compounds called the Pandemic Response Box(®) was used and four compounds were identified: alexidine and three non-commercial molecules. These compounds showed anti-biofilm activity, as well as alterations in fungal morphology and cell wall and plasma membrane structure. They also induced oxidative stress and mitochondrial membrane depolarization. In silico analysis revealed promising pharmacological parameters. These results suggest that these four compounds are potent candidates to be considered in future studies for the development of new approaches to treat mucormycosis.
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spelling pubmed-99595532023-02-26 Promising Antifungal Molecules against Mucormycosis Agents Identified from Pandemic Response Box(®): In Vitro and In Silico Analyses Xisto, Mariana Ingrid Dutra da Silva Rollin-Pinheiro, Rodrigo de Castro-Almeida, Yuri dos Santos-Freitas, Giulia Maria Pires Rochetti, Victor Pereira Borba-Santos, Luana Pereira da Silva Fontes, Yasmin Ferreira-Pereira, Antonio Rozental, Sonia Barreto-Bergter, Eliana J Fungi (Basel) Article Mucormycosis is considered concerning invasive fungal infections due to its high mortality rates, difficult diagnosis and limited treatment approaches. Mucorales species are highly resistant to many antifungal agents and the search for alternatives is an urgent need. In the present study, a library with 400 compounds called the Pandemic Response Box(®) was used and four compounds were identified: alexidine and three non-commercial molecules. These compounds showed anti-biofilm activity, as well as alterations in fungal morphology and cell wall and plasma membrane structure. They also induced oxidative stress and mitochondrial membrane depolarization. In silico analysis revealed promising pharmacological parameters. These results suggest that these four compounds are potent candidates to be considered in future studies for the development of new approaches to treat mucormycosis. MDPI 2023-01-31 /pmc/articles/PMC9959553/ /pubmed/36836302 http://dx.doi.org/10.3390/jof9020187 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xisto, Mariana Ingrid Dutra da Silva
Rollin-Pinheiro, Rodrigo
de Castro-Almeida, Yuri
dos Santos-Freitas, Giulia Maria Pires
Rochetti, Victor Pereira
Borba-Santos, Luana Pereira
da Silva Fontes, Yasmin
Ferreira-Pereira, Antonio
Rozental, Sonia
Barreto-Bergter, Eliana
Promising Antifungal Molecules against Mucormycosis Agents Identified from Pandemic Response Box(®): In Vitro and In Silico Analyses
title Promising Antifungal Molecules against Mucormycosis Agents Identified from Pandemic Response Box(®): In Vitro and In Silico Analyses
title_full Promising Antifungal Molecules against Mucormycosis Agents Identified from Pandemic Response Box(®): In Vitro and In Silico Analyses
title_fullStr Promising Antifungal Molecules against Mucormycosis Agents Identified from Pandemic Response Box(®): In Vitro and In Silico Analyses
title_full_unstemmed Promising Antifungal Molecules against Mucormycosis Agents Identified from Pandemic Response Box(®): In Vitro and In Silico Analyses
title_short Promising Antifungal Molecules against Mucormycosis Agents Identified from Pandemic Response Box(®): In Vitro and In Silico Analyses
title_sort promising antifungal molecules against mucormycosis agents identified from pandemic response box(®): in vitro and in silico analyses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959553/
https://www.ncbi.nlm.nih.gov/pubmed/36836302
http://dx.doi.org/10.3390/jof9020187
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