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ALP-Based Biosensors Employing Electrodes Modified with Carbon Nanomaterials for Pesticides Detection
Due to the growing presence of pesticides in the environment and in food, the concern of their impact on human health is increasing. Therefore, the development of fast and reliable detection methods is needed. Enzymatic inhibition-based biosensors represent a good alternative for replacing the more...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959578/ https://www.ncbi.nlm.nih.gov/pubmed/36838520 http://dx.doi.org/10.3390/molecules28041532 |
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author | Gianvittorio, Stefano Gualandi, Isacco Tonelli, Domenica |
author_facet | Gianvittorio, Stefano Gualandi, Isacco Tonelli, Domenica |
author_sort | Gianvittorio, Stefano |
collection | PubMed |
description | Due to the growing presence of pesticides in the environment and in food, the concern of their impact on human health is increasing. Therefore, the development of fast and reliable detection methods is needed. Enzymatic inhibition-based biosensors represent a good alternative for replacing the more complicated and time-consuming traditional methods (chromatography, spectrophotometry, etc.). This paper describes the development of an electrochemical biosensor exploiting alkaline phosphatase as the biological recognition element and a chemically modified glassy carbon electrode as the transducer. The biosensor was prepared modifying the GCE surface by a mixture of Multi-Walled-Carbon-Nanotubes (MWCNTs) and Electrochemically-Reduced-Graphene-Oxide (ERGO) followed by the immobilization of the enzyme by cross-linking with bovine serum albumin and glutaraldehyde. The inhibition of the biosensor response caused by pesticides was established using 2-phospho-L-ascorbic acid as the enzymatic substrate, whose dephosphorylation reaction produces ascorbic acid (AA). The MWCNTs/ERGO mixture shows a synergic effect in terms of increased sensitivity and decreased overpotential for AA oxidation. The response of the biosensor to the herbicide 2,4-dichloro-phenoxy-acetic-acid was evaluated and resulted in the concentration range 0.04–24 nM, with a limit of the detection of 16 pM. The determination of other pesticides was also achieved. The re-usability of the electrode was demonstrated by performing a washing procedure. |
format | Online Article Text |
id | pubmed-9959578 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99595782023-02-26 ALP-Based Biosensors Employing Electrodes Modified with Carbon Nanomaterials for Pesticides Detection Gianvittorio, Stefano Gualandi, Isacco Tonelli, Domenica Molecules Article Due to the growing presence of pesticides in the environment and in food, the concern of their impact on human health is increasing. Therefore, the development of fast and reliable detection methods is needed. Enzymatic inhibition-based biosensors represent a good alternative for replacing the more complicated and time-consuming traditional methods (chromatography, spectrophotometry, etc.). This paper describes the development of an electrochemical biosensor exploiting alkaline phosphatase as the biological recognition element and a chemically modified glassy carbon electrode as the transducer. The biosensor was prepared modifying the GCE surface by a mixture of Multi-Walled-Carbon-Nanotubes (MWCNTs) and Electrochemically-Reduced-Graphene-Oxide (ERGO) followed by the immobilization of the enzyme by cross-linking with bovine serum albumin and glutaraldehyde. The inhibition of the biosensor response caused by pesticides was established using 2-phospho-L-ascorbic acid as the enzymatic substrate, whose dephosphorylation reaction produces ascorbic acid (AA). The MWCNTs/ERGO mixture shows a synergic effect in terms of increased sensitivity and decreased overpotential for AA oxidation. The response of the biosensor to the herbicide 2,4-dichloro-phenoxy-acetic-acid was evaluated and resulted in the concentration range 0.04–24 nM, with a limit of the detection of 16 pM. The determination of other pesticides was also achieved. The re-usability of the electrode was demonstrated by performing a washing procedure. MDPI 2023-02-05 /pmc/articles/PMC9959578/ /pubmed/36838520 http://dx.doi.org/10.3390/molecules28041532 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gianvittorio, Stefano Gualandi, Isacco Tonelli, Domenica ALP-Based Biosensors Employing Electrodes Modified with Carbon Nanomaterials for Pesticides Detection |
title | ALP-Based Biosensors Employing Electrodes Modified with Carbon Nanomaterials for Pesticides Detection |
title_full | ALP-Based Biosensors Employing Electrodes Modified with Carbon Nanomaterials for Pesticides Detection |
title_fullStr | ALP-Based Biosensors Employing Electrodes Modified with Carbon Nanomaterials for Pesticides Detection |
title_full_unstemmed | ALP-Based Biosensors Employing Electrodes Modified with Carbon Nanomaterials for Pesticides Detection |
title_short | ALP-Based Biosensors Employing Electrodes Modified with Carbon Nanomaterials for Pesticides Detection |
title_sort | alp-based biosensors employing electrodes modified with carbon nanomaterials for pesticides detection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959578/ https://www.ncbi.nlm.nih.gov/pubmed/36838520 http://dx.doi.org/10.3390/molecules28041532 |
work_keys_str_mv | AT gianvittoriostefano alpbasedbiosensorsemployingelectrodesmodifiedwithcarbonnanomaterialsforpesticidesdetection AT gualandiisacco alpbasedbiosensorsemployingelectrodesmodifiedwithcarbonnanomaterialsforpesticidesdetection AT tonellidomenica alpbasedbiosensorsemployingelectrodesmodifiedwithcarbonnanomaterialsforpesticidesdetection |