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RBD and Spike DNA-Based Immunization in Rabbits Elicited IgG Avidity Maturation and High Neutralizing Antibody Responses against SARS-CoV-2

Neutralizing antibodies (nAbs) are a critical part of coronavirus disease 2019 (COVID-19) research as they are used to gain insight into the immune response to severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) infections. Among the technologies available for generating nAbs, DNA-b...

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Autores principales: da Costa, Hernan H. M., Orts, Diego J. B., Moura, Andrew D., Duarte-Neto, Amaro N., Cirqueira, Cinthya S., Réssio, Rodrigo A., Kanamura, Cristina T., Miguita, Karen, Ferreira, Jerenice E., Santos, Raimunda T. M., Adriani, Patricia P., Cunha-Junior, Jair P., Astray, Renato M., Catarino, Regina M., Lancelotti, Marcelo, Prudencio, Carlos R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959588/
https://www.ncbi.nlm.nih.gov/pubmed/36851769
http://dx.doi.org/10.3390/v15020555
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author da Costa, Hernan H. M.
Orts, Diego J. B.
Moura, Andrew D.
Duarte-Neto, Amaro N.
Cirqueira, Cinthya S.
Réssio, Rodrigo A.
Kanamura, Cristina T.
Miguita, Karen
Ferreira, Jerenice E.
Santos, Raimunda T. M.
Adriani, Patricia P.
Cunha-Junior, Jair P.
Astray, Renato M.
Catarino, Regina M.
Lancelotti, Marcelo
Prudencio, Carlos R.
author_facet da Costa, Hernan H. M.
Orts, Diego J. B.
Moura, Andrew D.
Duarte-Neto, Amaro N.
Cirqueira, Cinthya S.
Réssio, Rodrigo A.
Kanamura, Cristina T.
Miguita, Karen
Ferreira, Jerenice E.
Santos, Raimunda T. M.
Adriani, Patricia P.
Cunha-Junior, Jair P.
Astray, Renato M.
Catarino, Regina M.
Lancelotti, Marcelo
Prudencio, Carlos R.
author_sort da Costa, Hernan H. M.
collection PubMed
description Neutralizing antibodies (nAbs) are a critical part of coronavirus disease 2019 (COVID-19) research as they are used to gain insight into the immune response to severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) infections. Among the technologies available for generating nAbs, DNA-based immunization methods are an alternative to conventional protocols. In this pilot study, we investigated whether DNA-based immunization by needle injection in rabbits was a viable approach to produce a functional antibody response. We demonstrated that three doses of DNA plasmid carrying the gene encoding the full-length spike protein (S) or the receptor binding domain (RBD) of SARS-CoV-2 induced a time-dependent increase in IgG antibody avidity maturation. Moreover, the IgG antibodies displayed high cross neutralization by live SARS-CoV-2 and pseudoviruses neutralization assays. Thus, we established a simple, low cost and feasible DNA-based immunization protocol in rabbits that elicited high IgG avidity maturation and nAbs production against SARS-CoV-2, highlighting the importance of DNA-based platforms for developing new immunization strategies against SARS-CoV-2 and future emerging epidemics.
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spelling pubmed-99595882023-02-26 RBD and Spike DNA-Based Immunization in Rabbits Elicited IgG Avidity Maturation and High Neutralizing Antibody Responses against SARS-CoV-2 da Costa, Hernan H. M. Orts, Diego J. B. Moura, Andrew D. Duarte-Neto, Amaro N. Cirqueira, Cinthya S. Réssio, Rodrigo A. Kanamura, Cristina T. Miguita, Karen Ferreira, Jerenice E. Santos, Raimunda T. M. Adriani, Patricia P. Cunha-Junior, Jair P. Astray, Renato M. Catarino, Regina M. Lancelotti, Marcelo Prudencio, Carlos R. Viruses Brief Report Neutralizing antibodies (nAbs) are a critical part of coronavirus disease 2019 (COVID-19) research as they are used to gain insight into the immune response to severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) infections. Among the technologies available for generating nAbs, DNA-based immunization methods are an alternative to conventional protocols. In this pilot study, we investigated whether DNA-based immunization by needle injection in rabbits was a viable approach to produce a functional antibody response. We demonstrated that three doses of DNA plasmid carrying the gene encoding the full-length spike protein (S) or the receptor binding domain (RBD) of SARS-CoV-2 induced a time-dependent increase in IgG antibody avidity maturation. Moreover, the IgG antibodies displayed high cross neutralization by live SARS-CoV-2 and pseudoviruses neutralization assays. Thus, we established a simple, low cost and feasible DNA-based immunization protocol in rabbits that elicited high IgG avidity maturation and nAbs production against SARS-CoV-2, highlighting the importance of DNA-based platforms for developing new immunization strategies against SARS-CoV-2 and future emerging epidemics. MDPI 2023-02-17 /pmc/articles/PMC9959588/ /pubmed/36851769 http://dx.doi.org/10.3390/v15020555 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
da Costa, Hernan H. M.
Orts, Diego J. B.
Moura, Andrew D.
Duarte-Neto, Amaro N.
Cirqueira, Cinthya S.
Réssio, Rodrigo A.
Kanamura, Cristina T.
Miguita, Karen
Ferreira, Jerenice E.
Santos, Raimunda T. M.
Adriani, Patricia P.
Cunha-Junior, Jair P.
Astray, Renato M.
Catarino, Regina M.
Lancelotti, Marcelo
Prudencio, Carlos R.
RBD and Spike DNA-Based Immunization in Rabbits Elicited IgG Avidity Maturation and High Neutralizing Antibody Responses against SARS-CoV-2
title RBD and Spike DNA-Based Immunization in Rabbits Elicited IgG Avidity Maturation and High Neutralizing Antibody Responses against SARS-CoV-2
title_full RBD and Spike DNA-Based Immunization in Rabbits Elicited IgG Avidity Maturation and High Neutralizing Antibody Responses against SARS-CoV-2
title_fullStr RBD and Spike DNA-Based Immunization in Rabbits Elicited IgG Avidity Maturation and High Neutralizing Antibody Responses against SARS-CoV-2
title_full_unstemmed RBD and Spike DNA-Based Immunization in Rabbits Elicited IgG Avidity Maturation and High Neutralizing Antibody Responses against SARS-CoV-2
title_short RBD and Spike DNA-Based Immunization in Rabbits Elicited IgG Avidity Maturation and High Neutralizing Antibody Responses against SARS-CoV-2
title_sort rbd and spike dna-based immunization in rabbits elicited igg avidity maturation and high neutralizing antibody responses against sars-cov-2
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959588/
https://www.ncbi.nlm.nih.gov/pubmed/36851769
http://dx.doi.org/10.3390/v15020555
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