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A Genosensor Based on the Modification of a Microcantilever: A Review

When the free end of a microcantilever is modified by a genetic probe, this sensor can be used for a wider range of applications, such as for chemical analysis, biological testing, pharmaceutical screening, and environmental monitoring. In this paper, to clarify the preparation and detection process...

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Detalles Bibliográficos
Autores principales: Zhang, He, Yang, Shuang, Zeng, Jian, Li, Xin, Chuai, Rongyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959632/
https://www.ncbi.nlm.nih.gov/pubmed/36838127
http://dx.doi.org/10.3390/mi14020427
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author Zhang, He
Yang, Shuang
Zeng, Jian
Li, Xin
Chuai, Rongyan
author_facet Zhang, He
Yang, Shuang
Zeng, Jian
Li, Xin
Chuai, Rongyan
author_sort Zhang, He
collection PubMed
description When the free end of a microcantilever is modified by a genetic probe, this sensor can be used for a wider range of applications, such as for chemical analysis, biological testing, pharmaceutical screening, and environmental monitoring. In this paper, to clarify the preparation and detection process of a microcantilever sensor with genetic probe modification, the core procedures, such as probe immobilization, complementary hybridization, and signal extraction and processing, are combined and compared. Then, to reveal the microcantilever’s detection mechanism and analysis, the influencing factors of testing results, the theoretical research, including the deflection principle, the establishment and verification of a detection model, as well as environmental influencing factors are summarized. Next, to demonstrate the application results of the genetic-probe-modified sensors, based on the classification of detection targets, the application status of other substances except nucleic acid, virus, bacteria and cells is not introduced. Finally, by enumerating the application results of a genetic-probe-modified microcantilever combined with a microfluidic chip, the future development direction of this technology is surveyed. It is hoped that this review will contribute to the future design of a genetic-probe-modified microcantilever, with further exploration of the sensitive mechanism, optimization of the design and processing methods, expansion of the application fields, and promotion of practical application.
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spelling pubmed-99596322023-02-26 A Genosensor Based on the Modification of a Microcantilever: A Review Zhang, He Yang, Shuang Zeng, Jian Li, Xin Chuai, Rongyan Micromachines (Basel) Review When the free end of a microcantilever is modified by a genetic probe, this sensor can be used for a wider range of applications, such as for chemical analysis, biological testing, pharmaceutical screening, and environmental monitoring. In this paper, to clarify the preparation and detection process of a microcantilever sensor with genetic probe modification, the core procedures, such as probe immobilization, complementary hybridization, and signal extraction and processing, are combined and compared. Then, to reveal the microcantilever’s detection mechanism and analysis, the influencing factors of testing results, the theoretical research, including the deflection principle, the establishment and verification of a detection model, as well as environmental influencing factors are summarized. Next, to demonstrate the application results of the genetic-probe-modified sensors, based on the classification of detection targets, the application status of other substances except nucleic acid, virus, bacteria and cells is not introduced. Finally, by enumerating the application results of a genetic-probe-modified microcantilever combined with a microfluidic chip, the future development direction of this technology is surveyed. It is hoped that this review will contribute to the future design of a genetic-probe-modified microcantilever, with further exploration of the sensitive mechanism, optimization of the design and processing methods, expansion of the application fields, and promotion of practical application. MDPI 2023-02-10 /pmc/articles/PMC9959632/ /pubmed/36838127 http://dx.doi.org/10.3390/mi14020427 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Zhang, He
Yang, Shuang
Zeng, Jian
Li, Xin
Chuai, Rongyan
A Genosensor Based on the Modification of a Microcantilever: A Review
title A Genosensor Based on the Modification of a Microcantilever: A Review
title_full A Genosensor Based on the Modification of a Microcantilever: A Review
title_fullStr A Genosensor Based on the Modification of a Microcantilever: A Review
title_full_unstemmed A Genosensor Based on the Modification of a Microcantilever: A Review
title_short A Genosensor Based on the Modification of a Microcantilever: A Review
title_sort genosensor based on the modification of a microcantilever: a review
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959632/
https://www.ncbi.nlm.nih.gov/pubmed/36838127
http://dx.doi.org/10.3390/mi14020427
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