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Ferulic Acid Treats Gastric Ulcer via Suppressing Oxidative Stress and Inflammation

(1) Background: The aim of the present study was to evaluate the gastroprotective potential of ferulic acid (FA) on indomethacin-induced gastric ulcers in rats with macroscopic and microscopic examinations along with biochemical assays. (2) Methods: After 24 h starvation, the ulcer was induced in ma...

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Autores principales: Ermis, Aleyna, Aritici Colak, Gozde, Acikel-Elmas, Merve, Arbak, Serap, Kolgazi, Meltem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959638/
https://www.ncbi.nlm.nih.gov/pubmed/36836745
http://dx.doi.org/10.3390/life13020388
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author Ermis, Aleyna
Aritici Colak, Gozde
Acikel-Elmas, Merve
Arbak, Serap
Kolgazi, Meltem
author_facet Ermis, Aleyna
Aritici Colak, Gozde
Acikel-Elmas, Merve
Arbak, Serap
Kolgazi, Meltem
author_sort Ermis, Aleyna
collection PubMed
description (1) Background: The aim of the present study was to evaluate the gastroprotective potential of ferulic acid (FA) on indomethacin-induced gastric ulcers in rats with macroscopic and microscopic examinations along with biochemical assays. (2) Methods: After 24 h starvation, the ulcer was induced in male Sprague-Dawley rats by subcutaneous indomethacin (25 mg/kg) injection. Fifteen minutes after ulcer induction, rats were treated with either tween 80 or FA. FA was given by oral gavage at 100 mg/kg, 250 mg/kg, and 500 mg/kg. In the fourth hour, rats were euthanized and collected gastric samples were evaluated macroscopically and microscopically. Antioxidant parameters including malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), and inflammatory parameters comprising of myeloperoxidase (MPO), Tumor Necrosis Factor (TNF)-α, Interleukin (IL)-1β, IL-6 and Nuclear Factor Kappa-B (NF-κB) p65 levels were also determined. (3) Results: Indomethacin injection significantly increased the macroscopic and microscopic scores. In addition, it increased the gastric MDA, MPO, TNF-α, IL-1β, IL-6, and NF-κB p65 levels but reduced SOD and GSH content. Treatment with FA significantly improved the gastric injury macroscopically and microscopically. Moreover, FA displayed a marked decrease in the gastric levels of MDA, MPO, TNF-α, IL-1β, IL-6, and NF-κB p65 and a significant increase in SOD and GSH compared to the INDO group. Ultimately, 250 mg/kg FA was determined as the most effective dose. (4) Conclusion: Our results revealed that FA has a gastroprotective effect against indomethacin-induced gastric ulcers in rats due to its antioxidant and anti-inflammatory properties. As a result, FA may be a potential treatment choice for gastric ulcers.
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spelling pubmed-99596382023-02-26 Ferulic Acid Treats Gastric Ulcer via Suppressing Oxidative Stress and Inflammation Ermis, Aleyna Aritici Colak, Gozde Acikel-Elmas, Merve Arbak, Serap Kolgazi, Meltem Life (Basel) Article (1) Background: The aim of the present study was to evaluate the gastroprotective potential of ferulic acid (FA) on indomethacin-induced gastric ulcers in rats with macroscopic and microscopic examinations along with biochemical assays. (2) Methods: After 24 h starvation, the ulcer was induced in male Sprague-Dawley rats by subcutaneous indomethacin (25 mg/kg) injection. Fifteen minutes after ulcer induction, rats were treated with either tween 80 or FA. FA was given by oral gavage at 100 mg/kg, 250 mg/kg, and 500 mg/kg. In the fourth hour, rats were euthanized and collected gastric samples were evaluated macroscopically and microscopically. Antioxidant parameters including malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), and inflammatory parameters comprising of myeloperoxidase (MPO), Tumor Necrosis Factor (TNF)-α, Interleukin (IL)-1β, IL-6 and Nuclear Factor Kappa-B (NF-κB) p65 levels were also determined. (3) Results: Indomethacin injection significantly increased the macroscopic and microscopic scores. In addition, it increased the gastric MDA, MPO, TNF-α, IL-1β, IL-6, and NF-κB p65 levels but reduced SOD and GSH content. Treatment with FA significantly improved the gastric injury macroscopically and microscopically. Moreover, FA displayed a marked decrease in the gastric levels of MDA, MPO, TNF-α, IL-1β, IL-6, and NF-κB p65 and a significant increase in SOD and GSH compared to the INDO group. Ultimately, 250 mg/kg FA was determined as the most effective dose. (4) Conclusion: Our results revealed that FA has a gastroprotective effect against indomethacin-induced gastric ulcers in rats due to its antioxidant and anti-inflammatory properties. As a result, FA may be a potential treatment choice for gastric ulcers. MDPI 2023-01-31 /pmc/articles/PMC9959638/ /pubmed/36836745 http://dx.doi.org/10.3390/life13020388 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ermis, Aleyna
Aritici Colak, Gozde
Acikel-Elmas, Merve
Arbak, Serap
Kolgazi, Meltem
Ferulic Acid Treats Gastric Ulcer via Suppressing Oxidative Stress and Inflammation
title Ferulic Acid Treats Gastric Ulcer via Suppressing Oxidative Stress and Inflammation
title_full Ferulic Acid Treats Gastric Ulcer via Suppressing Oxidative Stress and Inflammation
title_fullStr Ferulic Acid Treats Gastric Ulcer via Suppressing Oxidative Stress and Inflammation
title_full_unstemmed Ferulic Acid Treats Gastric Ulcer via Suppressing Oxidative Stress and Inflammation
title_short Ferulic Acid Treats Gastric Ulcer via Suppressing Oxidative Stress and Inflammation
title_sort ferulic acid treats gastric ulcer via suppressing oxidative stress and inflammation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959638/
https://www.ncbi.nlm.nih.gov/pubmed/36836745
http://dx.doi.org/10.3390/life13020388
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