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Effects of Pb(II) and Zn(II) Contamination on Adsorption, Desorption and Degradation of Cry1Ac Toxin Identical to Bt Transgenic Poplar in Black Soil

Bt transgenic white poplar has been commercially planted in China since 2002, and it showed obvious insect resistance in the field. However, the ecological risk of planting Bt transgenic poplar in a field contaminated with heavy metals has received little attention. The effects of Pb(II) and Zn(II)...

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Autores principales: Wang, Yongji, Zhou, Xueyong, Zhang, Fenguo, Zhang, Lihong, Yang, Pingguo, Maimaitiniyazi, Rehanguli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959839/
https://www.ncbi.nlm.nih.gov/pubmed/36850965
http://dx.doi.org/10.3390/toxics11020089
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author Wang, Yongji
Zhou, Xueyong
Zhang, Fenguo
Zhang, Lihong
Yang, Pingguo
Maimaitiniyazi, Rehanguli
author_facet Wang, Yongji
Zhou, Xueyong
Zhang, Fenguo
Zhang, Lihong
Yang, Pingguo
Maimaitiniyazi, Rehanguli
author_sort Wang, Yongji
collection PubMed
description Bt transgenic white poplar has been commercially planted in China since 2002, and it showed obvious insect resistance in the field. However, the ecological risk of planting Bt transgenic poplar in a field contaminated with heavy metals has received little attention. The effects of Pb(II) and Zn(II) contamination on the adsorption, desorption and degradation of Bt toxin identical to Bt transgenic poplar in black soil were studied. The results showed that the adsorption of Bt toxin was enhanced and the desorption of Bt toxin was inhibited in black soil by Pb(II) and Zn(II) at concentrations between 0 and 1 mmol/L, and the effect of Pb(II) on Bt toxin was greater than that of Zn(II). In the presence of heavy metal ions, the Cry1Ac toxin molecules are oriented with domain I toward soil particles through the metal ion bridge. The promoting mechanism of Bt toxin adsorption by heavy metal ions in black soil is mainly attributed to cation-controlled electrostatic attraction (CCEA), which is different from patch-controlled electrostatic attraction (PCEA). With the increase in soil concentration from 1 to 4 mg/mL, the adsorption amount of Bt toxin showed a downward trend, and both Pb(II) and Zn(II) had the maximal promotion effect when the soil concentration was 2 mg/mL. The promoting effect of Zn(II) on the adsorption of Bt toxin increased with the increased temperature (5–45 °C), but the promoting effect of Pb(II) was maximal at 25 °C. Both Pb(II) and Zn(II) affected the degradation characteristics of Bt toxin in black soil. For the lead-contaminated black soil, the residual amount of Bt toxin increased in the early stage but decreased in the later stage compared to the control soil. For the zinc-contaminated black soil, the residual amount of Bt toxin decreased compared to the control soil except between the second and tenth days. In this study, it was observed that Bt toxin was degraded rapidly in the early stage, followed by a large amount of released Bt toxin and slow degradation in the middle and late stages.
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spelling pubmed-99598392023-02-26 Effects of Pb(II) and Zn(II) Contamination on Adsorption, Desorption and Degradation of Cry1Ac Toxin Identical to Bt Transgenic Poplar in Black Soil Wang, Yongji Zhou, Xueyong Zhang, Fenguo Zhang, Lihong Yang, Pingguo Maimaitiniyazi, Rehanguli Toxics Article Bt transgenic white poplar has been commercially planted in China since 2002, and it showed obvious insect resistance in the field. However, the ecological risk of planting Bt transgenic poplar in a field contaminated with heavy metals has received little attention. The effects of Pb(II) and Zn(II) contamination on the adsorption, desorption and degradation of Bt toxin identical to Bt transgenic poplar in black soil were studied. The results showed that the adsorption of Bt toxin was enhanced and the desorption of Bt toxin was inhibited in black soil by Pb(II) and Zn(II) at concentrations between 0 and 1 mmol/L, and the effect of Pb(II) on Bt toxin was greater than that of Zn(II). In the presence of heavy metal ions, the Cry1Ac toxin molecules are oriented with domain I toward soil particles through the metal ion bridge. The promoting mechanism of Bt toxin adsorption by heavy metal ions in black soil is mainly attributed to cation-controlled electrostatic attraction (CCEA), which is different from patch-controlled electrostatic attraction (PCEA). With the increase in soil concentration from 1 to 4 mg/mL, the adsorption amount of Bt toxin showed a downward trend, and both Pb(II) and Zn(II) had the maximal promotion effect when the soil concentration was 2 mg/mL. The promoting effect of Zn(II) on the adsorption of Bt toxin increased with the increased temperature (5–45 °C), but the promoting effect of Pb(II) was maximal at 25 °C. Both Pb(II) and Zn(II) affected the degradation characteristics of Bt toxin in black soil. For the lead-contaminated black soil, the residual amount of Bt toxin increased in the early stage but decreased in the later stage compared to the control soil. For the zinc-contaminated black soil, the residual amount of Bt toxin decreased compared to the control soil except between the second and tenth days. In this study, it was observed that Bt toxin was degraded rapidly in the early stage, followed by a large amount of released Bt toxin and slow degradation in the middle and late stages. MDPI 2023-01-18 /pmc/articles/PMC9959839/ /pubmed/36850965 http://dx.doi.org/10.3390/toxics11020089 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Yongji
Zhou, Xueyong
Zhang, Fenguo
Zhang, Lihong
Yang, Pingguo
Maimaitiniyazi, Rehanguli
Effects of Pb(II) and Zn(II) Contamination on Adsorption, Desorption and Degradation of Cry1Ac Toxin Identical to Bt Transgenic Poplar in Black Soil
title Effects of Pb(II) and Zn(II) Contamination on Adsorption, Desorption and Degradation of Cry1Ac Toxin Identical to Bt Transgenic Poplar in Black Soil
title_full Effects of Pb(II) and Zn(II) Contamination on Adsorption, Desorption and Degradation of Cry1Ac Toxin Identical to Bt Transgenic Poplar in Black Soil
title_fullStr Effects of Pb(II) and Zn(II) Contamination on Adsorption, Desorption and Degradation of Cry1Ac Toxin Identical to Bt Transgenic Poplar in Black Soil
title_full_unstemmed Effects of Pb(II) and Zn(II) Contamination on Adsorption, Desorption and Degradation of Cry1Ac Toxin Identical to Bt Transgenic Poplar in Black Soil
title_short Effects of Pb(II) and Zn(II) Contamination on Adsorption, Desorption and Degradation of Cry1Ac Toxin Identical to Bt Transgenic Poplar in Black Soil
title_sort effects of pb(ii) and zn(ii) contamination on adsorption, desorption and degradation of cry1ac toxin identical to bt transgenic poplar in black soil
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959839/
https://www.ncbi.nlm.nih.gov/pubmed/36850965
http://dx.doi.org/10.3390/toxics11020089
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