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Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patients

The detection of Scedosporium/Lomentospora is still based on non-standardized low-sensitivity culture procedures. This fact is particularly worrying in patients with cystic fibrosis (CF), where these fungi are the second most common filamentous fungi isolated, because a poor and delayed diagnosis ca...

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Autores principales: Martin-Souto, Leire, Antoran, Aitziber, Areitio, Maialen, Aparicio-Fernandez, Leire, Martín-Gómez, María Teresa, Fernandez, Roberto, Astigarraga, Egoitz, Barreda-Gómez, Gabriel, Schwarz, Carsten, Rickerts, Volker, Hernando, Fernando L., Rementeria, Aitor, Buldain, Idoia, Ramirez-Garcia, Andoni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959861/
https://www.ncbi.nlm.nih.gov/pubmed/36836272
http://dx.doi.org/10.3390/jof9020158
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author Martin-Souto, Leire
Antoran, Aitziber
Areitio, Maialen
Aparicio-Fernandez, Leire
Martín-Gómez, María Teresa
Fernandez, Roberto
Astigarraga, Egoitz
Barreda-Gómez, Gabriel
Schwarz, Carsten
Rickerts, Volker
Hernando, Fernando L.
Rementeria, Aitor
Buldain, Idoia
Ramirez-Garcia, Andoni
author_facet Martin-Souto, Leire
Antoran, Aitziber
Areitio, Maialen
Aparicio-Fernandez, Leire
Martín-Gómez, María Teresa
Fernandez, Roberto
Astigarraga, Egoitz
Barreda-Gómez, Gabriel
Schwarz, Carsten
Rickerts, Volker
Hernando, Fernando L.
Rementeria, Aitor
Buldain, Idoia
Ramirez-Garcia, Andoni
author_sort Martin-Souto, Leire
collection PubMed
description The detection of Scedosporium/Lomentospora is still based on non-standardized low-sensitivity culture procedures. This fact is particularly worrying in patients with cystic fibrosis (CF), where these fungi are the second most common filamentous fungi isolated, because a poor and delayed diagnosis can worsen the prognosis of the disease. To contribute to the discovery of new diagnostic strategies, a rapid serological dot immunobinding assay (DIA) that allows the detection of serum IgG against Scedosporium/Lomentospora in less than 15 min was developed. A crude protein extract from the conidia and hyphae of Scedosporium boydii was employed as a fungal antigen. The DIA was evaluated using 303 CF serum samples (162 patients) grouped according to the detection of Scedosporium/Lomentospora in the respiratory sample by culture, obtaining a sensitivity and specificity of 90.48% and 79.30%, respectively; positive and negative predictive values of 54.81% and 96.77%, and an efficiency of 81.72%. The clinical factors associated with the results were also studied using a univariate and a multivariate analysis, which showed that Scedosporium/Lomentospora positive sputum, elevated anti-Aspergillus serum IgG and chronic Pseudomonas aeruginosa infection were significantly associated with a positive result in DIA, while Staphylococcus aureus positive sputum showed a negative association. In conclusion, the test developed can offer a complementary, rapid, simple and sensitive method to contribute to the diagnosis of Scedosporium/Lomentospora in patients with CF.
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spelling pubmed-99598612023-02-26 Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patients Martin-Souto, Leire Antoran, Aitziber Areitio, Maialen Aparicio-Fernandez, Leire Martín-Gómez, María Teresa Fernandez, Roberto Astigarraga, Egoitz Barreda-Gómez, Gabriel Schwarz, Carsten Rickerts, Volker Hernando, Fernando L. Rementeria, Aitor Buldain, Idoia Ramirez-Garcia, Andoni J Fungi (Basel) Article The detection of Scedosporium/Lomentospora is still based on non-standardized low-sensitivity culture procedures. This fact is particularly worrying in patients with cystic fibrosis (CF), where these fungi are the second most common filamentous fungi isolated, because a poor and delayed diagnosis can worsen the prognosis of the disease. To contribute to the discovery of new diagnostic strategies, a rapid serological dot immunobinding assay (DIA) that allows the detection of serum IgG against Scedosporium/Lomentospora in less than 15 min was developed. A crude protein extract from the conidia and hyphae of Scedosporium boydii was employed as a fungal antigen. The DIA was evaluated using 303 CF serum samples (162 patients) grouped according to the detection of Scedosporium/Lomentospora in the respiratory sample by culture, obtaining a sensitivity and specificity of 90.48% and 79.30%, respectively; positive and negative predictive values of 54.81% and 96.77%, and an efficiency of 81.72%. The clinical factors associated with the results were also studied using a univariate and a multivariate analysis, which showed that Scedosporium/Lomentospora positive sputum, elevated anti-Aspergillus serum IgG and chronic Pseudomonas aeruginosa infection were significantly associated with a positive result in DIA, while Staphylococcus aureus positive sputum showed a negative association. In conclusion, the test developed can offer a complementary, rapid, simple and sensitive method to contribute to the diagnosis of Scedosporium/Lomentospora in patients with CF. MDPI 2023-01-24 /pmc/articles/PMC9959861/ /pubmed/36836272 http://dx.doi.org/10.3390/jof9020158 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Martin-Souto, Leire
Antoran, Aitziber
Areitio, Maialen
Aparicio-Fernandez, Leire
Martín-Gómez, María Teresa
Fernandez, Roberto
Astigarraga, Egoitz
Barreda-Gómez, Gabriel
Schwarz, Carsten
Rickerts, Volker
Hernando, Fernando L.
Rementeria, Aitor
Buldain, Idoia
Ramirez-Garcia, Andoni
Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patients
title Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patients
title_full Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patients
title_fullStr Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patients
title_full_unstemmed Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patients
title_short Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patients
title_sort dot immunobinding assay for the rapid serodetection of scedosporium/lomentospora in cystic fibrosis patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959861/
https://www.ncbi.nlm.nih.gov/pubmed/36836272
http://dx.doi.org/10.3390/jof9020158
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