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Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patients
The detection of Scedosporium/Lomentospora is still based on non-standardized low-sensitivity culture procedures. This fact is particularly worrying in patients with cystic fibrosis (CF), where these fungi are the second most common filamentous fungi isolated, because a poor and delayed diagnosis ca...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959861/ https://www.ncbi.nlm.nih.gov/pubmed/36836272 http://dx.doi.org/10.3390/jof9020158 |
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author | Martin-Souto, Leire Antoran, Aitziber Areitio, Maialen Aparicio-Fernandez, Leire Martín-Gómez, María Teresa Fernandez, Roberto Astigarraga, Egoitz Barreda-Gómez, Gabriel Schwarz, Carsten Rickerts, Volker Hernando, Fernando L. Rementeria, Aitor Buldain, Idoia Ramirez-Garcia, Andoni |
author_facet | Martin-Souto, Leire Antoran, Aitziber Areitio, Maialen Aparicio-Fernandez, Leire Martín-Gómez, María Teresa Fernandez, Roberto Astigarraga, Egoitz Barreda-Gómez, Gabriel Schwarz, Carsten Rickerts, Volker Hernando, Fernando L. Rementeria, Aitor Buldain, Idoia Ramirez-Garcia, Andoni |
author_sort | Martin-Souto, Leire |
collection | PubMed |
description | The detection of Scedosporium/Lomentospora is still based on non-standardized low-sensitivity culture procedures. This fact is particularly worrying in patients with cystic fibrosis (CF), where these fungi are the second most common filamentous fungi isolated, because a poor and delayed diagnosis can worsen the prognosis of the disease. To contribute to the discovery of new diagnostic strategies, a rapid serological dot immunobinding assay (DIA) that allows the detection of serum IgG against Scedosporium/Lomentospora in less than 15 min was developed. A crude protein extract from the conidia and hyphae of Scedosporium boydii was employed as a fungal antigen. The DIA was evaluated using 303 CF serum samples (162 patients) grouped according to the detection of Scedosporium/Lomentospora in the respiratory sample by culture, obtaining a sensitivity and specificity of 90.48% and 79.30%, respectively; positive and negative predictive values of 54.81% and 96.77%, and an efficiency of 81.72%. The clinical factors associated with the results were also studied using a univariate and a multivariate analysis, which showed that Scedosporium/Lomentospora positive sputum, elevated anti-Aspergillus serum IgG and chronic Pseudomonas aeruginosa infection were significantly associated with a positive result in DIA, while Staphylococcus aureus positive sputum showed a negative association. In conclusion, the test developed can offer a complementary, rapid, simple and sensitive method to contribute to the diagnosis of Scedosporium/Lomentospora in patients with CF. |
format | Online Article Text |
id | pubmed-9959861 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99598612023-02-26 Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patients Martin-Souto, Leire Antoran, Aitziber Areitio, Maialen Aparicio-Fernandez, Leire Martín-Gómez, María Teresa Fernandez, Roberto Astigarraga, Egoitz Barreda-Gómez, Gabriel Schwarz, Carsten Rickerts, Volker Hernando, Fernando L. Rementeria, Aitor Buldain, Idoia Ramirez-Garcia, Andoni J Fungi (Basel) Article The detection of Scedosporium/Lomentospora is still based on non-standardized low-sensitivity culture procedures. This fact is particularly worrying in patients with cystic fibrosis (CF), where these fungi are the second most common filamentous fungi isolated, because a poor and delayed diagnosis can worsen the prognosis of the disease. To contribute to the discovery of new diagnostic strategies, a rapid serological dot immunobinding assay (DIA) that allows the detection of serum IgG against Scedosporium/Lomentospora in less than 15 min was developed. A crude protein extract from the conidia and hyphae of Scedosporium boydii was employed as a fungal antigen. The DIA was evaluated using 303 CF serum samples (162 patients) grouped according to the detection of Scedosporium/Lomentospora in the respiratory sample by culture, obtaining a sensitivity and specificity of 90.48% and 79.30%, respectively; positive and negative predictive values of 54.81% and 96.77%, and an efficiency of 81.72%. The clinical factors associated with the results were also studied using a univariate and a multivariate analysis, which showed that Scedosporium/Lomentospora positive sputum, elevated anti-Aspergillus serum IgG and chronic Pseudomonas aeruginosa infection were significantly associated with a positive result in DIA, while Staphylococcus aureus positive sputum showed a negative association. In conclusion, the test developed can offer a complementary, rapid, simple and sensitive method to contribute to the diagnosis of Scedosporium/Lomentospora in patients with CF. MDPI 2023-01-24 /pmc/articles/PMC9959861/ /pubmed/36836272 http://dx.doi.org/10.3390/jof9020158 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Martin-Souto, Leire Antoran, Aitziber Areitio, Maialen Aparicio-Fernandez, Leire Martín-Gómez, María Teresa Fernandez, Roberto Astigarraga, Egoitz Barreda-Gómez, Gabriel Schwarz, Carsten Rickerts, Volker Hernando, Fernando L. Rementeria, Aitor Buldain, Idoia Ramirez-Garcia, Andoni Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patients |
title | Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patients |
title_full | Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patients |
title_fullStr | Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patients |
title_full_unstemmed | Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patients |
title_short | Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patients |
title_sort | dot immunobinding assay for the rapid serodetection of scedosporium/lomentospora in cystic fibrosis patients |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9959861/ https://www.ncbi.nlm.nih.gov/pubmed/36836272 http://dx.doi.org/10.3390/jof9020158 |
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