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Molecular Diagnosis and Vegetative Compatibility Group Analysis of Fusarium Wilt of Banana in Nepal

Fusarium wilt of banana (FWB), caused by Fusarium oxysporum f. sp. cubense (Foc), is the most important constraint of the banana industry globally. In Nepal, epidemics resembling FWB have been increasingly observed on the Malbhog cultivar in the past several years. However, the disease has not been...

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Autores principales: Pant, Bimala, Bai, Tingting, Du, Chanjuan, Baidya, Suraj, Magar, Prem Bahadur, Manandhar, Shrinkhala, Shrestha, Jiban, Dita, Miguel, Rouard, Mathieu, Fu, Gang, Zheng, Si-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9960093/
https://www.ncbi.nlm.nih.gov/pubmed/36836322
http://dx.doi.org/10.3390/jof9020208
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author Pant, Bimala
Bai, Tingting
Du, Chanjuan
Baidya, Suraj
Magar, Prem Bahadur
Manandhar, Shrinkhala
Shrestha, Jiban
Dita, Miguel
Rouard, Mathieu
Fu, Gang
Zheng, Si-Jun
author_facet Pant, Bimala
Bai, Tingting
Du, Chanjuan
Baidya, Suraj
Magar, Prem Bahadur
Manandhar, Shrinkhala
Shrestha, Jiban
Dita, Miguel
Rouard, Mathieu
Fu, Gang
Zheng, Si-Jun
author_sort Pant, Bimala
collection PubMed
description Fusarium wilt of banana (FWB), caused by Fusarium oxysporum f. sp. cubense (Foc), is the most important constraint of the banana industry globally. In Nepal, epidemics resembling FWB have been increasingly observed on the Malbhog cultivar in the past several years. However, the disease has not been officially reported yet, and consequently, little is known about the pathogen present across the country. In this study, we characterized 13 fungal strains isolated from banana plants of the Malbhog cultivar (Silk, AAB) showing symptoms similar to FWB in banana plantations in Nepal. All of the strains were typed as belonging to the F. oxysporum and caused FWB symptoms when inoculated in the Malbhog and Cachaco (Bluggoe, ABB) cultivars. No symptoms were observed in the Williams cultivar (Cavendish, AAA). Vegetative compatibility group (VCG) analysis classified the strains as VCG 0124 or VCG 0125. PCR analyses conducted with primers specific for Foc race 1 (Foc R1) or Foc tropical race 4 (TR4) revealed that all the strains reacted positively for Foc R1 and none for TR4. Altogether, our results demonstrated that the pathogen populations causing FWB of the Malbhog cultivar in Nepal were Foc R1. This work reported, for the first time, the occurrence of FWB in Nepal. Further studies with larger Foc populations are needed to better understand disease epidemiology to design sustainable disease management strategies.
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spelling pubmed-99600932023-02-26 Molecular Diagnosis and Vegetative Compatibility Group Analysis of Fusarium Wilt of Banana in Nepal Pant, Bimala Bai, Tingting Du, Chanjuan Baidya, Suraj Magar, Prem Bahadur Manandhar, Shrinkhala Shrestha, Jiban Dita, Miguel Rouard, Mathieu Fu, Gang Zheng, Si-Jun J Fungi (Basel) Article Fusarium wilt of banana (FWB), caused by Fusarium oxysporum f. sp. cubense (Foc), is the most important constraint of the banana industry globally. In Nepal, epidemics resembling FWB have been increasingly observed on the Malbhog cultivar in the past several years. However, the disease has not been officially reported yet, and consequently, little is known about the pathogen present across the country. In this study, we characterized 13 fungal strains isolated from banana plants of the Malbhog cultivar (Silk, AAB) showing symptoms similar to FWB in banana plantations in Nepal. All of the strains were typed as belonging to the F. oxysporum and caused FWB symptoms when inoculated in the Malbhog and Cachaco (Bluggoe, ABB) cultivars. No symptoms were observed in the Williams cultivar (Cavendish, AAA). Vegetative compatibility group (VCG) analysis classified the strains as VCG 0124 or VCG 0125. PCR analyses conducted with primers specific for Foc race 1 (Foc R1) or Foc tropical race 4 (TR4) revealed that all the strains reacted positively for Foc R1 and none for TR4. Altogether, our results demonstrated that the pathogen populations causing FWB of the Malbhog cultivar in Nepal were Foc R1. This work reported, for the first time, the occurrence of FWB in Nepal. Further studies with larger Foc populations are needed to better understand disease epidemiology to design sustainable disease management strategies. MDPI 2023-02-05 /pmc/articles/PMC9960093/ /pubmed/36836322 http://dx.doi.org/10.3390/jof9020208 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pant, Bimala
Bai, Tingting
Du, Chanjuan
Baidya, Suraj
Magar, Prem Bahadur
Manandhar, Shrinkhala
Shrestha, Jiban
Dita, Miguel
Rouard, Mathieu
Fu, Gang
Zheng, Si-Jun
Molecular Diagnosis and Vegetative Compatibility Group Analysis of Fusarium Wilt of Banana in Nepal
title Molecular Diagnosis and Vegetative Compatibility Group Analysis of Fusarium Wilt of Banana in Nepal
title_full Molecular Diagnosis and Vegetative Compatibility Group Analysis of Fusarium Wilt of Banana in Nepal
title_fullStr Molecular Diagnosis and Vegetative Compatibility Group Analysis of Fusarium Wilt of Banana in Nepal
title_full_unstemmed Molecular Diagnosis and Vegetative Compatibility Group Analysis of Fusarium Wilt of Banana in Nepal
title_short Molecular Diagnosis and Vegetative Compatibility Group Analysis of Fusarium Wilt of Banana in Nepal
title_sort molecular diagnosis and vegetative compatibility group analysis of fusarium wilt of banana in nepal
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9960093/
https://www.ncbi.nlm.nih.gov/pubmed/36836322
http://dx.doi.org/10.3390/jof9020208
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