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Epidemiological Analysis of Avian Reovirus in China and Research on the Immune Protection of Different Genotype Strains from 2019 to 2020

Avian reovirus (ARV) is the primary pathogen responsible for viral arthritis. In this study, 2340 samples with suspected viral arthritis were collected from 2019 to 2020 in 16 provinces of China to investigate the prevalence of ARV in China and to characterize the molecular genetic evolution of epid...

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Autores principales: Liu, Dong, Zou, Zhong, Song, Shanshan, Liu, Hongxiang, Gong, Xiao, Li, Bin, Liu, Ping, Wang, Qunyi, Liu, Fengbo, Luan, Dongzu, Zhang, Xiang, Du, Yuanzhao, Jin, Meilin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9960544/
https://www.ncbi.nlm.nih.gov/pubmed/36851362
http://dx.doi.org/10.3390/vaccines11020485
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author Liu, Dong
Zou, Zhong
Song, Shanshan
Liu, Hongxiang
Gong, Xiao
Li, Bin
Liu, Ping
Wang, Qunyi
Liu, Fengbo
Luan, Dongzu
Zhang, Xiang
Du, Yuanzhao
Jin, Meilin
author_facet Liu, Dong
Zou, Zhong
Song, Shanshan
Liu, Hongxiang
Gong, Xiao
Li, Bin
Liu, Ping
Wang, Qunyi
Liu, Fengbo
Luan, Dongzu
Zhang, Xiang
Du, Yuanzhao
Jin, Meilin
author_sort Liu, Dong
collection PubMed
description Avian reovirus (ARV) is the primary pathogen responsible for viral arthritis. In this study, 2340 samples with suspected viral arthritis were collected from 2019 to 2020 in 16 provinces of China to investigate the prevalence of ARV in China and to characterize the molecular genetic evolution of epidemic strains. From 113 samples analyzed by RT-PCR, 46 strains of avian reovirus were successfully isolated and identified. The genetic evolution of the σC gene showed that 46 strains were distributed in 1–5 branches, with the largest number of strains in branches 1 and 2. The σC gene homology among the strains was low, with approximately 62% homology in branches 4 and 5 and about 55% in the remaining branches. The strains circulating during the ARV epidemic in different provinces were distributed in different branches. The SPF chickens were immunized with inactivated vaccines containing strains from branches 1 and 4 to analyze the cross-immune protection elicited by different branches of ARV strains. A challenge protection test was performed using strains in branches 1, 2, 4, and 5. Our results showed that inactivated vaccines containing strains from branches 1 and 4 could fully protect from strains in branches 1, 4, and 5. The results of this study revealed the genetic diversity among the endemic strains of ARV in China from 2019 to 2020. Each genotype strain elicited partial cross-protection, providing a scientific basis for the prevention and control of ARV.
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spelling pubmed-99605442023-02-26 Epidemiological Analysis of Avian Reovirus in China and Research on the Immune Protection of Different Genotype Strains from 2019 to 2020 Liu, Dong Zou, Zhong Song, Shanshan Liu, Hongxiang Gong, Xiao Li, Bin Liu, Ping Wang, Qunyi Liu, Fengbo Luan, Dongzu Zhang, Xiang Du, Yuanzhao Jin, Meilin Vaccines (Basel) Article Avian reovirus (ARV) is the primary pathogen responsible for viral arthritis. In this study, 2340 samples with suspected viral arthritis were collected from 2019 to 2020 in 16 provinces of China to investigate the prevalence of ARV in China and to characterize the molecular genetic evolution of epidemic strains. From 113 samples analyzed by RT-PCR, 46 strains of avian reovirus were successfully isolated and identified. The genetic evolution of the σC gene showed that 46 strains were distributed in 1–5 branches, with the largest number of strains in branches 1 and 2. The σC gene homology among the strains was low, with approximately 62% homology in branches 4 and 5 and about 55% in the remaining branches. The strains circulating during the ARV epidemic in different provinces were distributed in different branches. The SPF chickens were immunized with inactivated vaccines containing strains from branches 1 and 4 to analyze the cross-immune protection elicited by different branches of ARV strains. A challenge protection test was performed using strains in branches 1, 2, 4, and 5. Our results showed that inactivated vaccines containing strains from branches 1 and 4 could fully protect from strains in branches 1, 4, and 5. The results of this study revealed the genetic diversity among the endemic strains of ARV in China from 2019 to 2020. Each genotype strain elicited partial cross-protection, providing a scientific basis for the prevention and control of ARV. MDPI 2023-02-20 /pmc/articles/PMC9960544/ /pubmed/36851362 http://dx.doi.org/10.3390/vaccines11020485 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Dong
Zou, Zhong
Song, Shanshan
Liu, Hongxiang
Gong, Xiao
Li, Bin
Liu, Ping
Wang, Qunyi
Liu, Fengbo
Luan, Dongzu
Zhang, Xiang
Du, Yuanzhao
Jin, Meilin
Epidemiological Analysis of Avian Reovirus in China and Research on the Immune Protection of Different Genotype Strains from 2019 to 2020
title Epidemiological Analysis of Avian Reovirus in China and Research on the Immune Protection of Different Genotype Strains from 2019 to 2020
title_full Epidemiological Analysis of Avian Reovirus in China and Research on the Immune Protection of Different Genotype Strains from 2019 to 2020
title_fullStr Epidemiological Analysis of Avian Reovirus in China and Research on the Immune Protection of Different Genotype Strains from 2019 to 2020
title_full_unstemmed Epidemiological Analysis of Avian Reovirus in China and Research on the Immune Protection of Different Genotype Strains from 2019 to 2020
title_short Epidemiological Analysis of Avian Reovirus in China and Research on the Immune Protection of Different Genotype Strains from 2019 to 2020
title_sort epidemiological analysis of avian reovirus in china and research on the immune protection of different genotype strains from 2019 to 2020
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9960544/
https://www.ncbi.nlm.nih.gov/pubmed/36851362
http://dx.doi.org/10.3390/vaccines11020485
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