Cargando…

Biodegradation of an injectable treated dentin matrix hydrogel as a novel pulp capping agent for dentin regeneration

BACKGROUND: A novel injectable mixture termed treated dentin matrix hydrogel (TDMH) has been introduced for restoring dentin defect in DPC. However, no study evaluated its physiological biodegradation. Therefore, the present study aimed to assess scaffold homogeneity, mechanical properties and biode...

Descripción completa

Detalles Bibliográficos
Autores principales: Holiel, Ahmed A., Mustafa, Hossam M., Sedek, Eman M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9960635/
https://www.ncbi.nlm.nih.gov/pubmed/36841767
http://dx.doi.org/10.1186/s12903-023-02831-4
_version_ 1784895559550631936
author Holiel, Ahmed A.
Mustafa, Hossam M.
Sedek, Eman M.
author_facet Holiel, Ahmed A.
Mustafa, Hossam M.
Sedek, Eman M.
author_sort Holiel, Ahmed A.
collection PubMed
description BACKGROUND: A novel injectable mixture termed treated dentin matrix hydrogel (TDMH) has been introduced for restoring dentin defect in DPC. However, no study evaluated its physiological biodegradation. Therefore, the present study aimed to assess scaffold homogeneity, mechanical properties and biodegradability in vitro and in vivo and the regenerated dentin induced by TDMH as a novel pulp capping agent in human permanent teeth. METHODS: Three TDMH discs were weighted, and dry/wet ratios were calculated in four slices from each disc to evaluate homogeneity. Hydrogel discs were also analyzed in triplicate to measure the compressive strength using a universal testing machine. The in vitro degradation behavior of hydrogel in PBS at 37 °C for 2 months was also investigated by monitoring the percent weight change. Moreover, 20 intact fully erupted premolars were included for assessment of TDMH in vivo biodegradation when used as a novel injectable pulp capping agent. The capped teeth were divided into four equal groups according to extraction interval after 2-, 8-, 12- and 16-weeks, stained with hematoxylin–eosin for histological and histomorphometric evaluation. Statistical analysis was performed using F test (ANOVA) and post hoc test (p = 0.05). RESULTS: No statistical differences among hydrogel slices were detected with (p = 0.192) according to homogeneity. TDMH compression modulus was (30.45 ± 1.11 kPa). Hydrogel retained its shape well up to 4 weeks and after 8 weeks completely degraded. Histological analysis after 16 weeks showed a significant reduction in TDMH area and a simultaneous significant increase in the new dentin area. The mean values of TDMH were 58.8% ± 5.9 and 9.8% ± 3.3 at 2 and 16 weeks, while the new dentin occupied 9.5% ± 2.8 at 2 weeks and 82.9% ± 3.8 at 16 weeks. CONCLUSIONS: TDMH was homogenous and exhibited significant stability and almost completely recovered after excessive compression. TDMH generally maintained their bulk geometry throughout 7 weeks. The in vivo response to TDMH was characterized by extensive degradation of the hydrogel and dentin matrix particles and abundant formation of new dentin. The degradation rate of TDMH matched the rate of new dentin formation. Trial registration: PACTR201901866476410: 30/1/2019.
format Online
Article
Text
id pubmed-9960635
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-99606352023-02-26 Biodegradation of an injectable treated dentin matrix hydrogel as a novel pulp capping agent for dentin regeneration Holiel, Ahmed A. Mustafa, Hossam M. Sedek, Eman M. BMC Oral Health Research BACKGROUND: A novel injectable mixture termed treated dentin matrix hydrogel (TDMH) has been introduced for restoring dentin defect in DPC. However, no study evaluated its physiological biodegradation. Therefore, the present study aimed to assess scaffold homogeneity, mechanical properties and biodegradability in vitro and in vivo and the regenerated dentin induced by TDMH as a novel pulp capping agent in human permanent teeth. METHODS: Three TDMH discs were weighted, and dry/wet ratios were calculated in four slices from each disc to evaluate homogeneity. Hydrogel discs were also analyzed in triplicate to measure the compressive strength using a universal testing machine. The in vitro degradation behavior of hydrogel in PBS at 37 °C for 2 months was also investigated by monitoring the percent weight change. Moreover, 20 intact fully erupted premolars were included for assessment of TDMH in vivo biodegradation when used as a novel injectable pulp capping agent. The capped teeth were divided into four equal groups according to extraction interval after 2-, 8-, 12- and 16-weeks, stained with hematoxylin–eosin for histological and histomorphometric evaluation. Statistical analysis was performed using F test (ANOVA) and post hoc test (p = 0.05). RESULTS: No statistical differences among hydrogel slices were detected with (p = 0.192) according to homogeneity. TDMH compression modulus was (30.45 ± 1.11 kPa). Hydrogel retained its shape well up to 4 weeks and after 8 weeks completely degraded. Histological analysis after 16 weeks showed a significant reduction in TDMH area and a simultaneous significant increase in the new dentin area. The mean values of TDMH were 58.8% ± 5.9 and 9.8% ± 3.3 at 2 and 16 weeks, while the new dentin occupied 9.5% ± 2.8 at 2 weeks and 82.9% ± 3.8 at 16 weeks. CONCLUSIONS: TDMH was homogenous and exhibited significant stability and almost completely recovered after excessive compression. TDMH generally maintained their bulk geometry throughout 7 weeks. The in vivo response to TDMH was characterized by extensive degradation of the hydrogel and dentin matrix particles and abundant formation of new dentin. The degradation rate of TDMH matched the rate of new dentin formation. Trial registration: PACTR201901866476410: 30/1/2019. BioMed Central 2023-02-25 /pmc/articles/PMC9960635/ /pubmed/36841767 http://dx.doi.org/10.1186/s12903-023-02831-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Holiel, Ahmed A.
Mustafa, Hossam M.
Sedek, Eman M.
Biodegradation of an injectable treated dentin matrix hydrogel as a novel pulp capping agent for dentin regeneration
title Biodegradation of an injectable treated dentin matrix hydrogel as a novel pulp capping agent for dentin regeneration
title_full Biodegradation of an injectable treated dentin matrix hydrogel as a novel pulp capping agent for dentin regeneration
title_fullStr Biodegradation of an injectable treated dentin matrix hydrogel as a novel pulp capping agent for dentin regeneration
title_full_unstemmed Biodegradation of an injectable treated dentin matrix hydrogel as a novel pulp capping agent for dentin regeneration
title_short Biodegradation of an injectable treated dentin matrix hydrogel as a novel pulp capping agent for dentin regeneration
title_sort biodegradation of an injectable treated dentin matrix hydrogel as a novel pulp capping agent for dentin regeneration
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9960635/
https://www.ncbi.nlm.nih.gov/pubmed/36841767
http://dx.doi.org/10.1186/s12903-023-02831-4
work_keys_str_mv AT holielahmeda biodegradationofaninjectabletreateddentinmatrixhydrogelasanovelpulpcappingagentfordentinregeneration
AT mustafahossamm biodegradationofaninjectabletreateddentinmatrixhydrogelasanovelpulpcappingagentfordentinregeneration
AT sedekemanm biodegradationofaninjectabletreateddentinmatrixhydrogelasanovelpulpcappingagentfordentinregeneration