Cargando…
β-N-Methylamino-L-Alanine (BMAA) Modulates the Sympathetic Regulation and Homeostasis of Polyamines
The neurotoxin β-N-methylamino-L-alanine (BMAA) is a non-proteinogenic amino acid produced by cyanobacteria. Non-neuronal toxicity of BMAA is poorly studied with a reported increase in reactive oxygen species and a decrease in the antioxidant capacity of liver, kidney, and colorectal adenocarcinoma...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9960692/ https://www.ncbi.nlm.nih.gov/pubmed/36828455 http://dx.doi.org/10.3390/toxins15020141 |
_version_ | 1784895572633714688 |
---|---|
author | Shkodrova, Milena Mishonova, Milena Chichova, Mariela Sazdova, Iliyana Ilieva, Bilyana Doncheva-Stoimenova, Dilyana Raikova, Neli Keremidarska-Markova, Milena Gagov, Hristo |
author_facet | Shkodrova, Milena Mishonova, Milena Chichova, Mariela Sazdova, Iliyana Ilieva, Bilyana Doncheva-Stoimenova, Dilyana Raikova, Neli Keremidarska-Markova, Milena Gagov, Hristo |
author_sort | Shkodrova, Milena |
collection | PubMed |
description | The neurotoxin β-N-methylamino-L-alanine (BMAA) is a non-proteinogenic amino acid produced by cyanobacteria. Non-neuronal toxicity of BMAA is poorly studied with a reported increase in reactive oxygen species and a decrease in the antioxidant capacity of liver, kidney, and colorectal adenocarcinoma cells. The aim of this research is to study the toxicity of BMAA (0.1–1 mM) on mitochondria and submitochondrial particles with ATPase activity, on the semicarbazide-sensitive amino oxidases (SSAOs) activity of rat liver, and on an in vitro model containing functionally active excitable tissues—regularly contracting heart muscle preparation with a preserved autonomic innervation. For the first time the BMAA-dependent inhibition of SSAO activity, the elimination of the positive inotropic effect of adrenergic innervation, and the direct and reversible inhibition of adrenaline signaling in ventricular myocytes with 1 mM BMAA were observed. Additionally, it is confirmed that 1 mM BMAA can activate mitochondrial ATPase indirectly. It is concluded that a higher dose of BMAA may influence multiple physiological and pathological processes as it slows down the degradation of biogenic amines, downregulates the sympathetic neuromediation, and embarrasses the cell signaling of adrenergic receptors. |
format | Online Article Text |
id | pubmed-9960692 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99606922023-02-26 β-N-Methylamino-L-Alanine (BMAA) Modulates the Sympathetic Regulation and Homeostasis of Polyamines Shkodrova, Milena Mishonova, Milena Chichova, Mariela Sazdova, Iliyana Ilieva, Bilyana Doncheva-Stoimenova, Dilyana Raikova, Neli Keremidarska-Markova, Milena Gagov, Hristo Toxins (Basel) Article The neurotoxin β-N-methylamino-L-alanine (BMAA) is a non-proteinogenic amino acid produced by cyanobacteria. Non-neuronal toxicity of BMAA is poorly studied with a reported increase in reactive oxygen species and a decrease in the antioxidant capacity of liver, kidney, and colorectal adenocarcinoma cells. The aim of this research is to study the toxicity of BMAA (0.1–1 mM) on mitochondria and submitochondrial particles with ATPase activity, on the semicarbazide-sensitive amino oxidases (SSAOs) activity of rat liver, and on an in vitro model containing functionally active excitable tissues—regularly contracting heart muscle preparation with a preserved autonomic innervation. For the first time the BMAA-dependent inhibition of SSAO activity, the elimination of the positive inotropic effect of adrenergic innervation, and the direct and reversible inhibition of adrenaline signaling in ventricular myocytes with 1 mM BMAA were observed. Additionally, it is confirmed that 1 mM BMAA can activate mitochondrial ATPase indirectly. It is concluded that a higher dose of BMAA may influence multiple physiological and pathological processes as it slows down the degradation of biogenic amines, downregulates the sympathetic neuromediation, and embarrasses the cell signaling of adrenergic receptors. MDPI 2023-02-09 /pmc/articles/PMC9960692/ /pubmed/36828455 http://dx.doi.org/10.3390/toxins15020141 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shkodrova, Milena Mishonova, Milena Chichova, Mariela Sazdova, Iliyana Ilieva, Bilyana Doncheva-Stoimenova, Dilyana Raikova, Neli Keremidarska-Markova, Milena Gagov, Hristo β-N-Methylamino-L-Alanine (BMAA) Modulates the Sympathetic Regulation and Homeostasis of Polyamines |
title | β-N-Methylamino-L-Alanine (BMAA) Modulates the Sympathetic Regulation and Homeostasis of Polyamines |
title_full | β-N-Methylamino-L-Alanine (BMAA) Modulates the Sympathetic Regulation and Homeostasis of Polyamines |
title_fullStr | β-N-Methylamino-L-Alanine (BMAA) Modulates the Sympathetic Regulation and Homeostasis of Polyamines |
title_full_unstemmed | β-N-Methylamino-L-Alanine (BMAA) Modulates the Sympathetic Regulation and Homeostasis of Polyamines |
title_short | β-N-Methylamino-L-Alanine (BMAA) Modulates the Sympathetic Regulation and Homeostasis of Polyamines |
title_sort | β-n-methylamino-l-alanine (bmaa) modulates the sympathetic regulation and homeostasis of polyamines |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9960692/ https://www.ncbi.nlm.nih.gov/pubmed/36828455 http://dx.doi.org/10.3390/toxins15020141 |
work_keys_str_mv | AT shkodrovamilena bnmethylaminolalaninebmaamodulatesthesympatheticregulationandhomeostasisofpolyamines AT mishonovamilena bnmethylaminolalaninebmaamodulatesthesympatheticregulationandhomeostasisofpolyamines AT chichovamariela bnmethylaminolalaninebmaamodulatesthesympatheticregulationandhomeostasisofpolyamines AT sazdovailiyana bnmethylaminolalaninebmaamodulatesthesympatheticregulationandhomeostasisofpolyamines AT ilievabilyana bnmethylaminolalaninebmaamodulatesthesympatheticregulationandhomeostasisofpolyamines AT donchevastoimenovadilyana bnmethylaminolalaninebmaamodulatesthesympatheticregulationandhomeostasisofpolyamines AT raikovaneli bnmethylaminolalaninebmaamodulatesthesympatheticregulationandhomeostasisofpolyamines AT keremidarskamarkovamilena bnmethylaminolalaninebmaamodulatesthesympatheticregulationandhomeostasisofpolyamines AT gagovhristo bnmethylaminolalaninebmaamodulatesthesympatheticregulationandhomeostasisofpolyamines |