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Larches of Kuzhanovo Have a Unique Mutation in the atpF–atpH Intergenic Spacer

The larches of Kuzhanovo (Larix sibirica Ledeb.) are protected trees with a round crown growing in the Southern Urals. In 2020 vandals sawed the sapwood of these trees, which exposed the problem of insufficient conservation measures. Their origin and genetic characteristics have been of particular i...

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Autores principales: Artyukhin, Alexander, Sharifyanova, Yuliya, Krivosheev, Mikhail M., Mikhaylova, Elena V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9960809/
https://www.ncbi.nlm.nih.gov/pubmed/36835349
http://dx.doi.org/10.3390/ijms24043939
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author Artyukhin, Alexander
Sharifyanova, Yuliya
Krivosheev, Mikhail M.
Mikhaylova, Elena V.
author_facet Artyukhin, Alexander
Sharifyanova, Yuliya
Krivosheev, Mikhail M.
Mikhaylova, Elena V.
author_sort Artyukhin, Alexander
collection PubMed
description The larches of Kuzhanovo (Larix sibirica Ledeb.) are protected trees with a round crown growing in the Southern Urals. In 2020 vandals sawed the sapwood of these trees, which exposed the problem of insufficient conservation measures. Their origin and genetic characteristics have been of particular interest to breeders and scientists. The larches of Kuzhanovo were screened for polymorphisms using SSR and ISSR analyses and the sequencing of genetic markers and genes GIGANTEA and mTERF, associated with wider crown shape. A unique mutation was discovered in the atpF–atpH intergenic spacer of all protected trees, but it was absent in some of their descendants and larches with similar crown shape. Mutations were discovered in the rpoC1 and mTERF genes of all samples. Flow cytometry did not reveal any changes in genome size. Our results suggest that the unique phenotype arose from point mutations in L. sibirica, but they are yet to be found in the nuclear genome. The concurrent mutations in the rpoC1 and mTERF genes may indicate that the round crown shape originates from the Southern Urals. The atpF–atpH and rpoC1 genetic markers are not common in studies of Larix sp., but their wider use could help to establish the origin of these endangered plants. The discovery of the unique atpF–atpH mutation also allows for stronger conservation and crime detection efforts.
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spelling pubmed-99608092023-02-26 Larches of Kuzhanovo Have a Unique Mutation in the atpF–atpH Intergenic Spacer Artyukhin, Alexander Sharifyanova, Yuliya Krivosheev, Mikhail M. Mikhaylova, Elena V. Int J Mol Sci Article The larches of Kuzhanovo (Larix sibirica Ledeb.) are protected trees with a round crown growing in the Southern Urals. In 2020 vandals sawed the sapwood of these trees, which exposed the problem of insufficient conservation measures. Their origin and genetic characteristics have been of particular interest to breeders and scientists. The larches of Kuzhanovo were screened for polymorphisms using SSR and ISSR analyses and the sequencing of genetic markers and genes GIGANTEA and mTERF, associated with wider crown shape. A unique mutation was discovered in the atpF–atpH intergenic spacer of all protected trees, but it was absent in some of their descendants and larches with similar crown shape. Mutations were discovered in the rpoC1 and mTERF genes of all samples. Flow cytometry did not reveal any changes in genome size. Our results suggest that the unique phenotype arose from point mutations in L. sibirica, but they are yet to be found in the nuclear genome. The concurrent mutations in the rpoC1 and mTERF genes may indicate that the round crown shape originates from the Southern Urals. The atpF–atpH and rpoC1 genetic markers are not common in studies of Larix sp., but their wider use could help to establish the origin of these endangered plants. The discovery of the unique atpF–atpH mutation also allows for stronger conservation and crime detection efforts. MDPI 2023-02-15 /pmc/articles/PMC9960809/ /pubmed/36835349 http://dx.doi.org/10.3390/ijms24043939 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Artyukhin, Alexander
Sharifyanova, Yuliya
Krivosheev, Mikhail M.
Mikhaylova, Elena V.
Larches of Kuzhanovo Have a Unique Mutation in the atpF–atpH Intergenic Spacer
title Larches of Kuzhanovo Have a Unique Mutation in the atpF–atpH Intergenic Spacer
title_full Larches of Kuzhanovo Have a Unique Mutation in the atpF–atpH Intergenic Spacer
title_fullStr Larches of Kuzhanovo Have a Unique Mutation in the atpF–atpH Intergenic Spacer
title_full_unstemmed Larches of Kuzhanovo Have a Unique Mutation in the atpF–atpH Intergenic Spacer
title_short Larches of Kuzhanovo Have a Unique Mutation in the atpF–atpH Intergenic Spacer
title_sort larches of kuzhanovo have a unique mutation in the atpf–atph intergenic spacer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9960809/
https://www.ncbi.nlm.nih.gov/pubmed/36835349
http://dx.doi.org/10.3390/ijms24043939
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