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Interactions between DMPC Model Membranes, the Drug Naproxen, and the Saponin β-Aescin

In this study, the interplay among the phospholipid 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) as a model membrane, the nonsteroidal anti-inflammatory drug naproxen, and the saponin [Formula: see text]-aescin are investigated. The naproxen amount was fixed to 10 mol%, and the saponin amount...

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Autores principales: Hägerbäumer, Pia, Gräbitz-Bräuer, Friederike, Annegarn, Marco, Dargel, Carina, Stank, Tim Julian, Bizien, Thomas, Hellweg, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9960855/
https://www.ncbi.nlm.nih.gov/pubmed/36839701
http://dx.doi.org/10.3390/pharmaceutics15020379
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author Hägerbäumer, Pia
Gräbitz-Bräuer, Friederike
Annegarn, Marco
Dargel, Carina
Stank, Tim Julian
Bizien, Thomas
Hellweg, Thomas
author_facet Hägerbäumer, Pia
Gräbitz-Bräuer, Friederike
Annegarn, Marco
Dargel, Carina
Stank, Tim Julian
Bizien, Thomas
Hellweg, Thomas
author_sort Hägerbäumer, Pia
collection PubMed
description In this study, the interplay among the phospholipid 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) as a model membrane, the nonsteroidal anti-inflammatory drug naproxen, and the saponin [Formula: see text]-aescin are investigated. The naproxen amount was fixed to 10 mol%, and the saponin amount varies from 0.0 to 1.0 mol%. Both substances are common ingredients in pharmaceutics; therefore, it is important to obtain deeper knowledge of their impact on lipid membranes. The size and properties of the DMPC model membrane upon naproxen and aescin addition were characterized with differential scanning calorimetry (DSC), small- and wide-angle X-ray scattering (SAXS, WAXS), and photon correlation spectroscopy (PCS) in a temperature-dependent study. The interaction of all substances was dependent on the lipid phase state, which itself depends on the lipid’s main phase transition temperature [Formula: see text]. The incorporation of naproxen and aescin distorted the lipid membrane structure and lowers [Formula: see text]. Below [Formula: see text] , the DMPC–naproxen–aescin mixtures showed a vesicle structure, and the insertion of naproxen and aescin influenced neither the lipid chain–chain correlation distance nor the membrane thickness. Above [Formula: see text] , the insertion of both molecules instead induced the formation of correlated bilayers and a decrease in the chain–chain correlation distance. The presented data clearly confirm the interaction of naproxen and aescin with DMPC model membranes. Moreover, the incorporation of both additives into the model membranes is evidenced.
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spelling pubmed-99608552023-02-26 Interactions between DMPC Model Membranes, the Drug Naproxen, and the Saponin β-Aescin Hägerbäumer, Pia Gräbitz-Bräuer, Friederike Annegarn, Marco Dargel, Carina Stank, Tim Julian Bizien, Thomas Hellweg, Thomas Pharmaceutics Article In this study, the interplay among the phospholipid 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) as a model membrane, the nonsteroidal anti-inflammatory drug naproxen, and the saponin [Formula: see text]-aescin are investigated. The naproxen amount was fixed to 10 mol%, and the saponin amount varies from 0.0 to 1.0 mol%. Both substances are common ingredients in pharmaceutics; therefore, it is important to obtain deeper knowledge of their impact on lipid membranes. The size and properties of the DMPC model membrane upon naproxen and aescin addition were characterized with differential scanning calorimetry (DSC), small- and wide-angle X-ray scattering (SAXS, WAXS), and photon correlation spectroscopy (PCS) in a temperature-dependent study. The interaction of all substances was dependent on the lipid phase state, which itself depends on the lipid’s main phase transition temperature [Formula: see text]. The incorporation of naproxen and aescin distorted the lipid membrane structure and lowers [Formula: see text]. Below [Formula: see text] , the DMPC–naproxen–aescin mixtures showed a vesicle structure, and the insertion of naproxen and aescin influenced neither the lipid chain–chain correlation distance nor the membrane thickness. Above [Formula: see text] , the insertion of both molecules instead induced the formation of correlated bilayers and a decrease in the chain–chain correlation distance. The presented data clearly confirm the interaction of naproxen and aescin with DMPC model membranes. Moreover, the incorporation of both additives into the model membranes is evidenced. MDPI 2023-01-22 /pmc/articles/PMC9960855/ /pubmed/36839701 http://dx.doi.org/10.3390/pharmaceutics15020379 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hägerbäumer, Pia
Gräbitz-Bräuer, Friederike
Annegarn, Marco
Dargel, Carina
Stank, Tim Julian
Bizien, Thomas
Hellweg, Thomas
Interactions between DMPC Model Membranes, the Drug Naproxen, and the Saponin β-Aescin
title Interactions between DMPC Model Membranes, the Drug Naproxen, and the Saponin β-Aescin
title_full Interactions between DMPC Model Membranes, the Drug Naproxen, and the Saponin β-Aescin
title_fullStr Interactions between DMPC Model Membranes, the Drug Naproxen, and the Saponin β-Aescin
title_full_unstemmed Interactions between DMPC Model Membranes, the Drug Naproxen, and the Saponin β-Aescin
title_short Interactions between DMPC Model Membranes, the Drug Naproxen, and the Saponin β-Aescin
title_sort interactions between dmpc model membranes, the drug naproxen, and the saponin β-aescin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9960855/
https://www.ncbi.nlm.nih.gov/pubmed/36839701
http://dx.doi.org/10.3390/pharmaceutics15020379
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