Cargando…

Benzimidazole Derivatives Suppress Fusarium Wilt Disease via Interaction with ERG6 of Fusarium equiseti and Activation of the Antioxidant Defense System of Pepper Plants

Sweet pepper (Capsicum annuum L.), also known as bell pepper, is one of the most widely grown vegetable crops worldwide. It is attacked by numerous phytopathogenic fungi, such as Fusarium equiseti, the causal agent of Fusarium wilt disease. In the current study, we proposed two benzimidazole derivat...

Descripción completa

Detalles Bibliográficos
Autores principales: El-Nagar, Asmaa, Elzaawely, Abdelnaser A., El-Zahaby, Hassan M., Xuan, Tran Dang, Khanh, Tran Dang, Gaber, Mohamed, El-Wakeil, Nadia, El-Sayed, Yusif, Nehela, Yasser
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9961032/
https://www.ncbi.nlm.nih.gov/pubmed/36836358
http://dx.doi.org/10.3390/jof9020244
_version_ 1784895655277232128
author El-Nagar, Asmaa
Elzaawely, Abdelnaser A.
El-Zahaby, Hassan M.
Xuan, Tran Dang
Khanh, Tran Dang
Gaber, Mohamed
El-Wakeil, Nadia
El-Sayed, Yusif
Nehela, Yasser
author_facet El-Nagar, Asmaa
Elzaawely, Abdelnaser A.
El-Zahaby, Hassan M.
Xuan, Tran Dang
Khanh, Tran Dang
Gaber, Mohamed
El-Wakeil, Nadia
El-Sayed, Yusif
Nehela, Yasser
author_sort El-Nagar, Asmaa
collection PubMed
description Sweet pepper (Capsicum annuum L.), also known as bell pepper, is one of the most widely grown vegetable crops worldwide. It is attacked by numerous phytopathogenic fungi, such as Fusarium equiseti, the causal agent of Fusarium wilt disease. In the current study, we proposed two benzimidazole derivatives, including 2-(2-hydroxyphenyl)-1-H benzimidazole (HPBI) and its aluminum complex (Al−HPBI complex), as potential control alternatives to F. equiseti. Our findings showed that both compounds demonstrated dose-dependent antifungal activity against F. equiseti in vitro and significantly suppressed disease development in pepper plants under greenhouse conditions. According to in silico analysis, the F. equiseti genome possesses a predicted Sterol 24-C-methyltransferase (FeEGR6) protein that shares a high degree of homology with EGR6 from F. oxysporum (FoEGR6). It is worth mentioning that molecular docking analysis confirmed that both compounds can interact with FeEGR6 from F. equiseti as well as FoEGR6 from F. oxysporum. Moreover, root application of HPBI and its aluminum complex significantly enhanced the enzymatic activities of guaiacol-dependent peroxidases (POX), polyphenol oxidase (PPO), and upregulated four antioxidant-related enzymes, including superoxide dismutase [Cu-Zn] (CaSOD-Cu), L-ascorbate peroxidase 1, cytosolic (CaAPX), glutathione reductase, chloroplastic (CaGR), and monodehydroascorbate reductase (CaMDHAR). Additionally, both benzimidazole derivatives induced the accumulation of total soluble phenolics and total soluble flavonoids. Collectively, these findings suggest that the application of HPBI and Al−HPBI complex induce both enzymatic and nonenzymatic antioxidant defense machinery.
format Online
Article
Text
id pubmed-9961032
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-99610322023-02-26 Benzimidazole Derivatives Suppress Fusarium Wilt Disease via Interaction with ERG6 of Fusarium equiseti and Activation of the Antioxidant Defense System of Pepper Plants El-Nagar, Asmaa Elzaawely, Abdelnaser A. El-Zahaby, Hassan M. Xuan, Tran Dang Khanh, Tran Dang Gaber, Mohamed El-Wakeil, Nadia El-Sayed, Yusif Nehela, Yasser J Fungi (Basel) Article Sweet pepper (Capsicum annuum L.), also known as bell pepper, is one of the most widely grown vegetable crops worldwide. It is attacked by numerous phytopathogenic fungi, such as Fusarium equiseti, the causal agent of Fusarium wilt disease. In the current study, we proposed two benzimidazole derivatives, including 2-(2-hydroxyphenyl)-1-H benzimidazole (HPBI) and its aluminum complex (Al−HPBI complex), as potential control alternatives to F. equiseti. Our findings showed that both compounds demonstrated dose-dependent antifungal activity against F. equiseti in vitro and significantly suppressed disease development in pepper plants under greenhouse conditions. According to in silico analysis, the F. equiseti genome possesses a predicted Sterol 24-C-methyltransferase (FeEGR6) protein that shares a high degree of homology with EGR6 from F. oxysporum (FoEGR6). It is worth mentioning that molecular docking analysis confirmed that both compounds can interact with FeEGR6 from F. equiseti as well as FoEGR6 from F. oxysporum. Moreover, root application of HPBI and its aluminum complex significantly enhanced the enzymatic activities of guaiacol-dependent peroxidases (POX), polyphenol oxidase (PPO), and upregulated four antioxidant-related enzymes, including superoxide dismutase [Cu-Zn] (CaSOD-Cu), L-ascorbate peroxidase 1, cytosolic (CaAPX), glutathione reductase, chloroplastic (CaGR), and monodehydroascorbate reductase (CaMDHAR). Additionally, both benzimidazole derivatives induced the accumulation of total soluble phenolics and total soluble flavonoids. Collectively, these findings suggest that the application of HPBI and Al−HPBI complex induce both enzymatic and nonenzymatic antioxidant defense machinery. MDPI 2023-02-12 /pmc/articles/PMC9961032/ /pubmed/36836358 http://dx.doi.org/10.3390/jof9020244 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
El-Nagar, Asmaa
Elzaawely, Abdelnaser A.
El-Zahaby, Hassan M.
Xuan, Tran Dang
Khanh, Tran Dang
Gaber, Mohamed
El-Wakeil, Nadia
El-Sayed, Yusif
Nehela, Yasser
Benzimidazole Derivatives Suppress Fusarium Wilt Disease via Interaction with ERG6 of Fusarium equiseti and Activation of the Antioxidant Defense System of Pepper Plants
title Benzimidazole Derivatives Suppress Fusarium Wilt Disease via Interaction with ERG6 of Fusarium equiseti and Activation of the Antioxidant Defense System of Pepper Plants
title_full Benzimidazole Derivatives Suppress Fusarium Wilt Disease via Interaction with ERG6 of Fusarium equiseti and Activation of the Antioxidant Defense System of Pepper Plants
title_fullStr Benzimidazole Derivatives Suppress Fusarium Wilt Disease via Interaction with ERG6 of Fusarium equiseti and Activation of the Antioxidant Defense System of Pepper Plants
title_full_unstemmed Benzimidazole Derivatives Suppress Fusarium Wilt Disease via Interaction with ERG6 of Fusarium equiseti and Activation of the Antioxidant Defense System of Pepper Plants
title_short Benzimidazole Derivatives Suppress Fusarium Wilt Disease via Interaction with ERG6 of Fusarium equiseti and Activation of the Antioxidant Defense System of Pepper Plants
title_sort benzimidazole derivatives suppress fusarium wilt disease via interaction with erg6 of fusarium equiseti and activation of the antioxidant defense system of pepper plants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9961032/
https://www.ncbi.nlm.nih.gov/pubmed/36836358
http://dx.doi.org/10.3390/jof9020244
work_keys_str_mv AT elnagarasmaa benzimidazolederivativessuppressfusariumwiltdiseaseviainteractionwitherg6offusariumequisetiandactivationoftheantioxidantdefensesystemofpepperplants
AT elzaawelyabdelnasera benzimidazolederivativessuppressfusariumwiltdiseaseviainteractionwitherg6offusariumequisetiandactivationoftheantioxidantdefensesystemofpepperplants
AT elzahabyhassanm benzimidazolederivativessuppressfusariumwiltdiseaseviainteractionwitherg6offusariumequisetiandactivationoftheantioxidantdefensesystemofpepperplants
AT xuantrandang benzimidazolederivativessuppressfusariumwiltdiseaseviainteractionwitherg6offusariumequisetiandactivationoftheantioxidantdefensesystemofpepperplants
AT khanhtrandang benzimidazolederivativessuppressfusariumwiltdiseaseviainteractionwitherg6offusariumequisetiandactivationoftheantioxidantdefensesystemofpepperplants
AT gabermohamed benzimidazolederivativessuppressfusariumwiltdiseaseviainteractionwitherg6offusariumequisetiandactivationoftheantioxidantdefensesystemofpepperplants
AT elwakeilnadia benzimidazolederivativessuppressfusariumwiltdiseaseviainteractionwitherg6offusariumequisetiandactivationoftheantioxidantdefensesystemofpepperplants
AT elsayedyusif benzimidazolederivativessuppressfusariumwiltdiseaseviainteractionwitherg6offusariumequisetiandactivationoftheantioxidantdefensesystemofpepperplants
AT nehelayasser benzimidazolederivativessuppressfusariumwiltdiseaseviainteractionwitherg6offusariumequisetiandactivationoftheantioxidantdefensesystemofpepperplants