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Mammalian Oocyte Analysis by MALDI MSI with Wet-Interface Matrix Deposition Technique
Oocytes are a special kind of biological material. Here, the individual variability of a single cell is important. It means that the opportunity to obtain information about the lipid content from the analysis of a single cell is significant. In our study, we present a method for lipid analysis based...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9961235/ https://www.ncbi.nlm.nih.gov/pubmed/36837109 http://dx.doi.org/10.3390/ma16041479 |
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author | Bodzon-Kulakowska, Anna Młodawska, Wiesława Mielczarek, Przemyslaw Lachowicz, Dorota Suder, Piotr Smoluch, Marek |
author_facet | Bodzon-Kulakowska, Anna Młodawska, Wiesława Mielczarek, Przemyslaw Lachowicz, Dorota Suder, Piotr Smoluch, Marek |
author_sort | Bodzon-Kulakowska, Anna |
collection | PubMed |
description | Oocytes are a special kind of biological material. Here, the individual variability of a single cell is important. It means that the opportunity to obtain information about the lipid content from the analysis of a single cell is significant. In our study, we present a method for lipid analysis based on the MALDI-based mass spectrometry imaging (MSI) approach. Our attention was paid to the sample preparation optimization with the aid of a wet-interface matrix deposition system (matrix spraying). Technical considerations of the sample preparation process, such as the number of matrix layers and the position of the spraying nozzle during the matrix deposition, are presented in the article. Additionally, we checked if changing the 2,5-dihydroxybenzoic acid (DHB) and 9-Aminoacridine (9AA) matrix concentration and their solvent composition may improve the analysis. Moreover, the comparison of paraformaldehyde-fixed versus nonfixed cell analysis was performed. We hope that our approach will be helpful for those working on lipid analyses in extraordinary material such as a single oocyte. Our study may also offer clues for anybody interested in single-cell analysis with the aid of MALDI mass spectrometry imaging and the wet-interface matrix deposition method. |
format | Online Article Text |
id | pubmed-9961235 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99612352023-02-26 Mammalian Oocyte Analysis by MALDI MSI with Wet-Interface Matrix Deposition Technique Bodzon-Kulakowska, Anna Młodawska, Wiesława Mielczarek, Przemyslaw Lachowicz, Dorota Suder, Piotr Smoluch, Marek Materials (Basel) Article Oocytes are a special kind of biological material. Here, the individual variability of a single cell is important. It means that the opportunity to obtain information about the lipid content from the analysis of a single cell is significant. In our study, we present a method for lipid analysis based on the MALDI-based mass spectrometry imaging (MSI) approach. Our attention was paid to the sample preparation optimization with the aid of a wet-interface matrix deposition system (matrix spraying). Technical considerations of the sample preparation process, such as the number of matrix layers and the position of the spraying nozzle during the matrix deposition, are presented in the article. Additionally, we checked if changing the 2,5-dihydroxybenzoic acid (DHB) and 9-Aminoacridine (9AA) matrix concentration and their solvent composition may improve the analysis. Moreover, the comparison of paraformaldehyde-fixed versus nonfixed cell analysis was performed. We hope that our approach will be helpful for those working on lipid analyses in extraordinary material such as a single oocyte. Our study may also offer clues for anybody interested in single-cell analysis with the aid of MALDI mass spectrometry imaging and the wet-interface matrix deposition method. MDPI 2023-02-09 /pmc/articles/PMC9961235/ /pubmed/36837109 http://dx.doi.org/10.3390/ma16041479 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bodzon-Kulakowska, Anna Młodawska, Wiesława Mielczarek, Przemyslaw Lachowicz, Dorota Suder, Piotr Smoluch, Marek Mammalian Oocyte Analysis by MALDI MSI with Wet-Interface Matrix Deposition Technique |
title | Mammalian Oocyte Analysis by MALDI MSI with Wet-Interface Matrix Deposition Technique |
title_full | Mammalian Oocyte Analysis by MALDI MSI with Wet-Interface Matrix Deposition Technique |
title_fullStr | Mammalian Oocyte Analysis by MALDI MSI with Wet-Interface Matrix Deposition Technique |
title_full_unstemmed | Mammalian Oocyte Analysis by MALDI MSI with Wet-Interface Matrix Deposition Technique |
title_short | Mammalian Oocyte Analysis by MALDI MSI with Wet-Interface Matrix Deposition Technique |
title_sort | mammalian oocyte analysis by maldi msi with wet-interface matrix deposition technique |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9961235/ https://www.ncbi.nlm.nih.gov/pubmed/36837109 http://dx.doi.org/10.3390/ma16041479 |
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